Anti-TMEM150C/Tentonin 3 (extracellular) Antibody | Alomone Labs APC-088 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-TMEM150C/Tentonin 3 (extracellular) Antibody

catalog :

APC-088

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of mouse brain membranes (lanes 1 and 3) and rat brain lysate (lanes 2 and 4):

image 2 :

Western blot analysis of human THP-1 monocytic leukemia cell line lysate (lanes 1 and 3) and human U-87 MG glioblastoma cell line lysate (2 and 4):

image 3 :

Expression of TMEM150C in rat dorsal root ganglia (DRG).

product information

CAT :

APC-088

SKU :

APC-088-CF_0.2 ml

Product Name :

Anti-TMEM150C/Tentonin 3 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

B5DFH9

Applications :

IFC IHC LCI WB

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PC088

Homology :

Mouse - 17 out of 18 amino acid residues identical Human - 16 out of 18 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, 1st loop.

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)EDDKILPLNSAARKSGVK, corresponding to amino acid residues 33 - 50 of rat TMEM150C

Product Page - Scientific background :

Transmembrane protein 150C (TMEM150C) also known as Tentonin-3 (TTN3), is a small protein composed of 249 amino acids comprising six transmembrane domains.TMEM150C which was first thought to be a mechanosensitive ion channel that confers a relatively slow inactivating current in proprioceptive neurons in mouse dorsal root ganglion (DRG)1. Later studies, however, showed that the mechanosensitive currents attributed to TMEM150C in different cell setups, could be attributed to the presence of endogenous Piezo1 channels2.Although, some controversy still remains, it is generally accepted that TMEM150C does not form a mechanosensitive channel per se, but rather it functions as a general regulator or sensor, of mechanosensitive channels like Piezo1, Piezo2 and TREK1 (K2P2.1) 2,3.TMEM150C has also been described in the aortic arch and nodose ganglia neurons, where it is involved in the mechanotransduction of arterial pressure to electrical signals in baroreceptors. Dysregulation of TMEM150C alters the sensitivity of the baroreflex, a cardiovascular reflex that is essential for blood pressure homeostasis, leading to diseases such as hypertension, stroke, and heart failure 4.Furthermore, recent evidence suggests that TMEM150C functions as a stretch-activated cation channel in pancreatic β cells, contributing to insulin secretion induced by high glucose levels and hypotonicity. Whether TMEM150C functions in this context as a stretch activated channel itself or whether it functions in conjunction with other proposed stretch-activated channels like TRPV4 or TRPV2, remains to be seen 3,5.

Applications may also work in :

IFC IHC LCI WB

Supplier :

Alomone Labs

Target :

Transmembrane Protein 150C, TTN3

Short Description :

A Rabbit Polyclonal antibody to TMEM150C/Tentonin 3 (extracellular).

Long Description :

Anti-TMEM150C/Tentonin 3 (extracellular) Antibody (#APC-088) is a highly specific antibody directed against an extracellular epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize TMEM150C from mouse, rat and human samples.

Negative Control :

BLP-PC088

Positive Control :

Synonyms :

Transmembrane Protein 150C, TTN3

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

TMEM150C

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:300

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage