Anti-Piezo1 (extracellular) Antibody | Alomone Labs APC-087 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Piezo1 (extracellular) Antibody

catalog :

APC-087

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 8

Reference
Swain S, Romac J, Vigna S, Liddle R. Piezo1-mediated stellate cell activation causes pressure-induced pancreatic fibrosis in mice. JCI Insight. 2022;7: pubmed publisher
Passini F, Jaeger P, Saab A, Hanlon S, Chittim N, Arlt M, et al. Shear-stress sensing by PIEZO1 regulates tendon stiffness in rodents and influences jumping performance in humans. Nat Biomed Eng. 2021;: pubmed publisher
Liu L, Zhang Q, Xiao S, Sun Z, Ding S, Chen Y, et al. Inhibition of Shear-Induced Platelet Aggregation by Xueshuantong via Targeting Piezo1 Channel-Mediated Ca2+ Signaling Pathway. Front Pharmacol. 2021;12:606245 pubmed publisher
Sugisawa E, Takayama Y, Takemura N, Kondo T, Hatakeyama S, Kumagai Y, et al. RNA Sensing by Gut Piezo1 Is Essential for Systemic Serotonin Synthesis. Cell. 2020;: pubmed publisher
John L, Ko N, Gokin A, Gokina N, Mandala M, Osol G. The Piezo1 cation channel mediates uterine artery shear stress mechanotransduction and vasodilation during rat pregnancy. Am J Physiol Heart Circ Physiol. 2018;315:H1019-H1026 pubmed publisher
Liu Q, Sun B, Zhao J, Wang Q, An F, Hu X, et al. Increased Piezo1 channel activity in interstitial Cajal-like cells induces bladder hyperactivity by functionally interacting with NCX1 in rats with cyclophosphamide-induced cystitis. Exp Mol Med. 2018;50:60 pubmed publisher
Romac J, Shahid R, Swain S, Vigna S, Liddle R. Piezo1 is a mechanically activated ion channel and mediates pressure induced pancreatitis. Nat Commun. 2018;9:1715 pubmed publisher
Copp S, Kim J, Ruiz Velasco V, Kaufman M. The mechano-gated channel inhibitor GsMTx4 reduces the exercise pressor reflex in rats with ligated femoral arteries. Am J Physiol Heart Circ Physiol. 2016;310:H1233-41 pubmed publisher

image

image 1 :

Western blot analysis of rat lung (lanes 1 and 3) and mouse lung (lanes 2 and 4) lysates: - 1,2. Anti-Piezo1 Antibody (#APC-087), (1:600).3,4. Anti-Piezo1 Antibody, preincubated with Piezo1 Blocking Peptide (#BLP-PC087).

image 2 :

Expression of Piezo1 in rat olfactory bulb - Immunohistochemical staining of rat olfactory bulb sections using Anti-Piezo1 Antibody (#APC-087). A. Piezo1 staining (green) appears in the neuropil with varying intensity within and between glomeruli. B. Nuclear staining (blue) using DAPI as the counterstain. C. Merged image of panels A and B.

product information

CAT :

APC-087

SKU :

APC-087-CF_0.2 ml

Product Name :

Anti-Piezo1 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q0KL00

Applications :

IF IFC IHC LCI WB

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PC087

Homology :

Mouse - 12/14 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

The peptide is located in an internal region of the rat Piezo1 (the exact topology of Piezo1 remains to be defined).

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)EDLKPQHRRHISIR, corresponding to amino acid residues 1863-1876 of rat Piezo1

Product Page - Scientific background :

Piezo1 protein is a family member of mixed cationic, non selective, directly mechanically activated ion channels found in higher eukaryotic cells. Piezo proteins are associated with physiological responses in cells that have been identified in many cell types and tissues in several species where mechanosensitivity plays a major role1,2.Two mammalian Piezo proteins were identified, piezo1 and piezo2 encoded by the fam38A and fam38B genes recpectively2. Piezo1 expression is detected in organs such as: bladder, colon, kidney, lung and skin3.The secondary structure and general length of Piezo proteins are quite conserved among species, and seem to form homotetrameric aggregates. Each subunit comprises between 24-32 putative transmembrane domains. The channels are constitutively active in liposomes and their activity does not require any other proteins1.Mechanosensitive proteins have been linked to various pathophysiological situations such as hearing and deafness, cardiac physiological and hypertrophic responses, and kidney function and disease2.Several studies have demonstrated that Piezo1 is essential for the removal of extra cells without apoptosis. Mutations in Piezo1 protein have been linked to the pathological response of red blood cells in a genetic disease called Xerocytosis. These finding suggest that Piezo1 plays a major role in cells' responses to mechanical stimuli1. In addition, Piezo1 plays a critical role in endothelial cell mechanotransduction which is essential for embryonic development. Knock-out model of Piezo1 affects the ability of endothelial cells to alter their alignment when exposed to shear stress. Furthermore, loss of Piezo1 in endothelial cells leads to deficits in stress fiber and cellular orientation in response to shear stress, therefore Piezo1mechanotransduction is linked to regulation of cell morphology4.

Applications may also work in :

IF IFC IHC LCI WB

Supplier :

Alomone Labs

Target :

Piezo-type mechanosensitive ion channel component 1, Protein FAM38A, Mib, Membrane protein induced by beta-amyloid treatment

Short Description :

A Rabbit Polyclonal Antibody to Piezo1 Channel

Long Description :

Anti-Piezo1 (extracellular) Antibody (#APC-087) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry and flow cytometry applications. It has been designed to recognize Piezo1 from rat and mouse samples. The antibody is unlikely to recognize the protein from human samples.

Negative Control :

BLP-PC087

Positive Control :

Synonyms :

Piezo-type mechanosensitive ion channel component 1, Protein FAM38A, Mib, Membrane protein induced by beta-amyloid treatment

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

PIEZO1

AB Specifictiy :

Not recommended for use with human samples.

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

KO-Validated :

yes

Cited Application :

IP IHC ICC LCI

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:600

more info or order :

Alomone Labs product webpage