Anti-Piezo1 (extracellular)-APC Antibody | Alomone Labs APC-087-APC product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Piezo1 (extracellular)-APC Antibody

catalog :

APC-087-APC

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

flow cytometry

more info or order :

Alomone Labs product webpage

image

image 1 :

Cell surface detection of Piezo1 by direct flow cytometry in live intact mouse J774 macrophage cells:

image 2 :

Cell surface detection of Piezo1 by direct flow cytometry in live intact mouse BV-2 microglia cells:

product information

CAT :

APC-087-APC

SKU :

APC-087-APC_50 mcl

Product Name :

Anti-Piezo1 (extracellular)-APC Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q0KL00

Applications :

FC LCI

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PC087

Homology :

Mouse - 12 out of 14 amino acids residues identical It won't recognize human Piezo1

Formulation :

PBS pH7.4, 1% BSA with 0.05% sodium azide

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

50 µl double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

1% BSA, 0.05% NaN3

Immunogen Location :

The peptide is located in an internal region of the rat Piezo1 (the exact topology of Piezo1 remains to be defined).

Label :

Allophycocyanin (APC).

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)EDLKPQHRRHISIR, corresponding to amino acid residues 1863-1876 of rat Piezo1

Product Page - Scientific background :

Piezo1 protein is a family member of mixed cationic, non selective, directly mechanically activated ion channels found in higher eukaryotic cells. Piezo proteins are associated with physiological responses in cells that have been identified in many cell types and tissues in several species where mechanosensitivity plays a major role1,2.Two mammalian Piezo proteins were identified, piezo1 and piezo2 encoded by the fam38A and fam38B genes recpectively2. Piezo1 expression is detected in organs such as: bladder, colon, kidney, lung and skin3.The secondary structure and general length of Piezo proteins are quite conserved among species, and seem to form homotetrameric aggregates. Each subunit comprises between 24-32 putative transmembrane domains. The channels are constitutively active in liposomes and their activity does not require any other proteins1.Mechanosensitive proteins have been linked to various pathophysiological situations such as hearing and deafness, cardiac physiological and hypertrophic responses, and kidney function and disease2.Several studies have demonstrated that Piezo1 is essential for the removal of extra cells without apoptosis. Mutations in Piezo1 protein have been linked to the pathological response of red blood cells in a genetic disease called Xerocytosis. These finding suggest that Piezo1 plays a major role in cells' responses to mechanical stimuli1. In addition, Piezo1 plays a critical role in endothelial cell mechanotransduction which is essential for embryonic development. Knock-out model of Piezo1 affects the ability of endothelial cells to alter their alignment when exposed to shear stress. Furthermore, loss of Piezo1 in endothelial cells leads to deficits in stress fiber and cellular orientation in response to shear stress, therefore Piezo1mechanotransduction is linked to regulation of cell morphology4.

Applications may also work in :

FC LCI

Supplier :

Alomone Labs

Target :

Piezo-type mechanosensitive ion channel component 1, Protein FAM38A, Mib, Membrane protein induced by beta-amyloid treatment

Short Description :

A Rabbit Polyclonal Antibody to Piezo1 (extracellular) conjugated to the fluorescent dye Allophycocyanin (APC)

Long Description :

Anti-Piezo1 (extracellular) Antibody (#APC-087) is a highly specific antibody directed against an extracellular epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry and live cell flow cytometry applications. It has been designed to recognize Piezo1 from mouse and rat samples. The antibody is will not recognize the protein from human samples. Anti-Piezo1 (extracellular)-APC Antibody (#APC-087-APC) is directly conjugated to the Allophycocyanin (APC) fluorophore. This conjugated antibody has been developed to be used in immunofluorescent applications such as direct flow cytometry and live cell imaging. It has been designed to recognize Piezo1 from mouse and rat samples.

Negative Control :

RIC-001-APC

Positive Control :

Synonyms :

Piezo-type mechanosensitive ion channel component 1, Protein FAM38A, Mib, Membrane protein induced by beta-amyloid treatment

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

PIEZO1

Form :

Lyophilized powder

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis (unlabeled antibody, #APC-087), and direct flow cytometry (labeled antibody).

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

Contact Alomone for information

more info or order :

Alomone Labs product webpage