Anti-CNGA1 Antibody | Alomone Labs APC-071 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-CNGA1 Antibody

catalog :

APC-071

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat eye lysate: - 1. Anti-CNGA1 Antibody (#APC-071), (1:200).2. Anti-CNGA1 Antibody, preincubated with CNGA1 Blocking Peptide (#BLP-PC071).

image 2 :

Peptide (C)NDNGSLSEESENED corresponding to amino acid residues 41-54 of rat CNGA1 (AccessionQ62927). Intracellular N-terminus.

image 3 :

Western blot analysis of rat brain membrane (lanes 1 and 5) mouse brain membrane (lanes 2 and 6) human acute lymphoblastic leukemia (MOLT-4) (lanes 3 and 7) and human colorectal adenocarcinoma (HT-29) cell lysates: - 1-4.Anti-LETM1Antibody (#ANT-183) (1:1500).5-8. Anti-LETM1 Antibody preincubated with the negative control antigen.

product information

CAT :

APC-071

SKU :

APC-071-CF_0.2 ml

Product Name :

Anti-CNGA1 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q62927

Applications :

Reactivity :

Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PC071

Homology :

Mouse - 13/14 amino acid residues identical; human - 9/14 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)NDNGSLSEESENED, corresponding to amino acid residues 41-54 of rat CNGA1

Product Page - Scientific background :

Cyclic nucleotide-gated (CNG) channels belong to the superfamily of voltage-gated ion channels. Although permeable to various ions such as the monovalent Na+ and K+ ions, and the divalent Ca2+ ion, they are gated by the intracellular binding of the cyclic nucleotides cAMP and cGMP and not by voltage per se (CNGs bind preferably to cGMP)1.Six channels form this subfamily: The A subunit (CNGA1-4) and the B subunit (CNGB1 and CNGB3). Functional entities are formed by the assembly of four subunits. Each subunit consists of six membrane spanning domains, where the fourth transmembrane domain is highly positively charged, a pore domain between transmembrane domains five and six, and intracellular N- and C-termini. The cyclic nucleotide binding domain is located in the C-terminal region and is responsible for the channel gating upon cyclic nucleotide binding1.These channels are highly expressed in retinal photoreceptors and olfactory neurons where their role has been extensively studied2. CNG channels have also been detected in brain, testis and kidney although their role in these tissues has yet to be unraveled2. When heterologously expressed all subunits with exception of CNGA4, CNGB1 and CNGB3 can form functional homomeric channels1. In rod photoreceptors, functional channels are composed of three CNGA1 subunits and one CNGB1 subunit3.Mutations in either of CNGA1 or CNGB1 genes are responsible for the onset of retinitis pigmentosa, a progressive degeneration of rod and cone receptors which is presented in part by night blindness3-4. As no knock out CNGA1 model systems exist, a transgenic mice line with lowered CNGA1 protein levels demonstrated symptoms which are reminiscent of retinitis pigmentosa, in agreement with the fact that a lack of CNGA1 is responsible for retinitis pigmentosa4.

Applications may also work in :

Supplier :

Alomone Labs

Target :

Cyclic nucleotide-gated cation channel 1, CNG channel alpha-1, CNG1, CNCG1, Rod photoreceptor cGMP-gated channel subunit alpha, RCNC1, RP49

Short Description :

A Rabbit Polyclonal Antibody to CNGA1 Channel

Long Description :

Anti-CNGA1 Antibody (#APC-071) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot analysis. It has been designed to recognize CNGA1 from human, rat, and mouse samples.

Negative Control :

BLP-PC071

Positive Control :

Synonyms :

Cyclic nucleotide-gated cation channel 1, CNG channel alpha-1, CNG1, CNCG1, Rod photoreceptor cGMP-gated channel subunit alpha, RCNC1, RP49

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

CNGA1

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage