Anti-sloβ3 (KCNMB3) (extracellular) Antibody | Alomone Labs APC-068 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-sloβ3 (KCNMB3) (extracellular) Antibody

catalog :

APC-068

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Alomone Labs product webpage

citations: 4

Reference
Briot J, Mailhot O, Bourdin B, Tétreault M, Najmanovich R, Parent L. A three-way inter-molecular network accounts for the CaVα2δ1-induced functional modulation of the pore-forming CaV1.2 subunit. J Biol Chem. 2018;293:7176-7188 pubmed publisher
Bourdin B, Briot J, Tétreault M, Sauve R, Parent L. Negatively charged residues in the first extracellular loop of the L-type CaV1.2 channel anchor the interaction with the CaV?2?1 auxiliary subunit. J Biol Chem. 2017;292:17236-17249 pubmed publisher
Segura E, Bourdin B, Tétreault M, Briot J, Allen B, Mayer G, et al. Proteolytic cleavage of the hydrophobic domain in the CaV?2?1 subunit improves assembly and activity of cardiac CaV1.2 channels. J Biol Chem. 2017;292:11109-11124 pubmed publisher
Kicinska A, Augustynek B, Kulawiak B, Jarmuszkiewicz W, Szewczyk A, Bednarczyk P. A large-conductance calcium-regulated K+ channel in human dermal fibroblast mitochondria. Biochem J. 2016;473:4457-4471 pubmed

image

image 1 :

Western blot analysis of mouse brain lysates (lanes 1 and 3) and rat pancreas membranes (lanes 2 and 4): - 1,2. Anti-sloβ3 (KCNMB3) (extracellular) Antibody (#APC-068), (1:1000).3,4. Anti-sloβ3 (KCNMB3) (extracellular) Antibody, preincubated with sloβ3/KCNMB3 (extracellular) Blocking Peptide (#BLP-PC068).

image 2 :

Expression of KCNMB3 in rat hippocampus. - Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-sloβ3 (KCNMB3) (extracellular) Antibody (#APC-068), (1:300), followed by goat anti-rabbit-Alexa-488. A. Staining in the hippocampal CA3 region, showed immunoreactivity (green) in cortical neurons (arrows). B. Pre-incubation of the antibody with sloβ3 (KCNMB3) (extracellular) Blocking Peptide (BLP-PC068), suppressed staining. Cell nuclei are stained with DAPI (blue).

product information

CAT :

APC-068

SKU :

APC-068-CF_0.2 ml

Product Name :

Anti-sloβ3 (KCNMB3) (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

A7VL23

Applications :

IHC WB

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PC068

Homology :

Mouse - 11/12 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)HYDEEAIRTNPK, corresponding to amino acid residues 134-145 of rat KCNMB3

Product Page - Scientific background :

Large-conductance, voltage- and Ca2+-activated K+ channels, also known as the BK channels, are widely expressed channels that couple changes in submembrane Ca2+ concentration to the regulation of electrical excitability1. BK channels are formed from four α-subunits arising from the Slowpoke (Slo) gene product2. In addition, in smooth muscle and cochlea, an accessory β-subunit can regulate BK channel gating profoundly. At present, four β subunits have been cloned in mammals. β subunits alter the Ca2+ sensitivity and gating kinetics of BK channels, greatly contributing to BK channel diversity. On the other hand, they modify the BK channel pharmacological properties, changing toxin binding and acting as receptors for drugs3.Regulatory β subunits share a putative membrane topology, with two transmembrane segments connected by a 120- residue extracellular loop and with NH2 and COOH terminals oriented toward the cytoplasm4. Each β subunit has a tissue-specific expression and modulates channel function uniquely which provides a major mechanism for diverse BK channel phenotypes in various tissues.β3 is highly expressed in kidney, heart, and brain5. The β3 gene (KCNMB3) associates with Slo1 α subunits and regulate BK channel function. In humans, the β3 gene contains four N-terminal alternative exons that produce four functionally distinct β3 subunits, β3a-d. Three variants, β3a-c, exhibit kinetically distinct inactivation behaviors6. A mutation in the β3 gene is linked to idiopathic epilepsy7.

Applications may also work in :

IHC WB

Supplier :

Alomone Labs

Target :

Ca2+-activated K+ channel subunit β3, BKCa β3, BK beta 3, Maxi K channel β3, Charybdotoxin receptor subunit beta-3

Short Description :

A Rabbit Polyclonal Antibody to slo?3 (KCNMB3) Subunit

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an extracellular epitope of rat sloβ3. Anti-sloβ3 (KCNMB3) (extracellular) Antibody (#APC-068) can be used in western blot analysis. It has been designed to recognize sloβ3 from rat and mouse samples.

Negative Control :

BLP-PC068

Positive Control :

Synonyms :

Ca2+-activated K+ channel subunit β3, BKCa β3, BK beta 3, Maxi K channel β3, Charybdotoxin receptor subunit beta-3

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

KCNMB3

AB Specifictiy :

Not recommended for human samples.

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:300

Application Dilutions: Western blot wb :

1:1000

more info or order :

Alomone Labs product webpage