Anti-KCNN4 (KCa3.1, SK4) Antibody | Alomone Labs APC-064 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-KCNN4 (KCa3.1, SK4) Antibody

catalog :

APC-064

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

Alomone Labs product webpage

citations: 9

Published Application/Species/Sample/Dilution	Reference
western blot; human; 1:500; loading ...; fig s2e	Centeio R, Ousingsawat J, Schreiber R, Kunzelmann K. CLCA1 Regulates Airway Mucus Production and Ion Secretion Through TMEM16A. Int J Mol Sci. 2021;22: pubmed publisher
	Bachmann M, Rossa A, Varanita T, Fioretti B, Biasutto L, Milenkovic S, et al. Pharmacological targeting of the mitochondrial calcium-dependent potassium channel KCa3.1 triggers cell death and reduces tumor growth and metastasis in vivo. Cell Death Dis. 2022;13:1055 pubmed publisher
	Pinilla E, S xe1 nchez A, Mart xed nez M, Mu xf1 oz M, Garc xed a Sacrist xe1 n A, K xf6 hler R, et al. Endothelial KCa 1.1 and KCa 3.1 channels mediate rat intrarenal artery endothelium-derived hyperpolarization response. Acta Physiol (Oxf). 2021;231:e13598 pubmed publisher
	Newton H, Gawali V, Chimote A, Lehn M, Palackdharry S, Hinrichs B, et al. PD1 blockade enhances K+ channel activity, Ca2+ signaling, and migratory ability in cytotoxic T lymphocytes of patients with head and neck cancer. J Immunother Cancer. 2020;8: pubmed publisher
	Liu Y, Kabakov A, Xie A, Shi G, Singh A, Sodha N, et al. Metabolic regulation of endothelial SK channels and human coronary microvascular function. Int J Cardiol. 2020;312:1-9 pubmed publisher
	Jin L, Lucente J, Nguyen H, Singh V, Singh L, Chavez M, et al. Repurposing the KCa3.1 inhibitor senicapoc for Alzheimer's disease. Ann Clin Transl Neurol. 2019;6:723-738 pubmed publisher
	Matsui M, Terasawa K, Kajikuri J, Kito H, Endo K, Jaikhan P, et al. Histone Deacetylases Enhance Ca2+-Activated K⁺ Channel KCa3.1 Expression in Murine Inflammatory CD4⁺ T Cells. Int J Mol Sci. 2018;19: pubmed publisher
	Thurber A, Nelson M, Frost C, Levin M, Brackenbury W, Kaplan D. IK channel activation increases tumor growth and induces differential behavioral responses in two breast epithelial cell lines. Oncotarget. 2017;8:42382-42397 pubmed publisher
	Klein H, Abu Arish A, Trinh N, Luo Y, Wiseman P, Hanrahan J, et al. Investigating CFTR and KCa3.1 Protein/Protein Interactions. PLoS ONE. 2016;11:e0153665 pubmed publisher

image

image 1 :

Western blot analysis of KCa3.1-transfected HEK-293 (lanes 1 and 3) and Human K562 chronic myelogenous leukemia (lanes 2 and 4) cells: - 1,2. Anti-KCNN4 (KCa3.1, SK4) Antibody (#APC-064), (1:200). 3,4. Anti-KCNN4 (KCa3.1, SK4) Antibody, preincubated with KCNN4/KCa3.1 Blocking Peptide (#BLP-PC064).

image 2 :

Western blot analysisof HEK-293-KCa3.1 (lanes 1 3) and K562 (lanes 2 4) cells: - 13.Anti-KCNN4 (KCa3.1 SK4)Antibody (#APC-064)(1:200).24. Anti-KCNN4 (KCa3.1 SK4) Antibody preincubated with the control peptide antigen.

image 3 :

Western blot analysis of human Jurkat T-cell leukemia cell line lysate (lanes 1 and 3) and human Colo 205 colon adenocarcinoma cell line lysate (lanes 2 and 4): - 12.Anti-ASCT1 (SLC1A4) Antibody(#ANT-081) (1:200).34. Anti-ASCT1 (SLC1A4) Antibody preincubated with the negative control antigen.

product information

CAT :

APC-064

SKU :

APC-064-CF_0.2 ml

Product Name :

Anti-KCNN4 (KCa3.1, SK4) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q9QYW1

Applications :

IC IF IHC IP WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PC064

Homology :

Mouse, human, pig - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminal domain

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

RQVRLKHRKLREQV(C), corresponding to amino acid residues 350-363 of rat KCNN4

Product Page - Scientific background :

KCa3.1 (KCNN4, SK4) is a member of the Ca2+ activated K+ channel family that shares the characteristic of being activated by intracellular Ca2+. The channel has an intermediate conductance, is voltage insensitive and is activated by Ca2+ in the submicromolar range. The channel has a similar topology to that of KV channels, that is, six transmembrane domains and intracellular N- and C-termini. KCa3.1 is widely expressed in epithelial, endothelial and cells of hematopoietic origin. In erythrocytes (red blood cells) it has been identified as the molecular correlate of the so-called Gardos channel. The functional role of the channel is to set the cell membrane potential at negative values so as to aid in the electrochemical transport of other ions such as Cl- and Ca2+. Indeed, KCa3.1 has a key role in sustaining the Ca2+ influx in activated T lymphocytes and in regulating Cl- secretion from colon epithelium. Therefore, specific blockers of the KCa3.1 channel have been proposed for the treatment of several diseases including autoimmune diseases, secretory diarrhea and sickle cell anemia.

Applications may also work in :

IC IF IHC IP WB

Supplier :

Alomone Labs

Target :

IKCa1, IK1, Intermediate conductance Ca2+-activated K+ channel protein 4, Gardos channel

Short Description :

A Rabbit Polyclonal Antibody to KCNN4 (KCa3.1, SK4) Channel

Long Description :

Anti-KCNN4 (KCa3.1, SK4) Antibody (#APC-064) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot analysis, immunocytochemistry, immunohistochemistry, and immunoprecipitation applications. It has been designed to recognize KCNN4 from human, rat, and mouse samples.

Negative Control :

BLP-PC064

Positive Control :

Synonyms :

IKCa1, IK1, Intermediate conductance Ca2+-activated K+ channel protein 4, Gardos channel

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

KCNN4

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IP IHC ICC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage