Anti-sloβ4 (KCNMB4) Antibody | Alomone Labs APC-061 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-sloβ4 (KCNMB4) Antibody

catalog :

APC-061

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 9

Reference
Sęk A, Kampa R, Kulawiak B, Szewczyk A, Bednarczyk P. Identification of the Large-Conductance Ca2+-Regulated Potassium Channel in Mitochondria of Human Bronchial Epithelial Cells. Molecules. 2021;26: pubmed publisher
Bertrand J, Schicht M, Stamer W, Baker D, Sherwood J, Lütjen Drecoll E, et al. The β4-Subunit of the Large-Conductance Potassium Ion Channel KCa1.1 Regulates Outflow Facility in Mice. Invest Ophthalmol Vis Sci. 2020;61:41 pubmed publisher
Zhao H, Xue Q, Li C, Wang Q, Han S, Zhou Y, et al. Upregulation of Beta4 subunit of BKCa channels in the anterior cingulate cortex contributes to mechanical allodynia associated anxiety-like behaviors. Mol Brain. 2020;13:22 pubmed publisher
Wang B, Wang France J, Li H, Sansom S. Furosemide reduces BK-αβ4-mediated K+ secretion in mice on an alkaline high-K+ diet. Am J Physiol Renal Physiol. 2019;316:F341-F350 pubmed publisher
Baker D, Pryce G, Visintin C, Sisay S, Bondarenko A, Vanessa Ho W, et al. Big conductance calcium-activated potassium channel openers control spasticity without sedation. Br J Pharmacol. 2017;174:2662-2681 pubmed publisher
Mamenko M, Boukelmoune N, Tomilin V, Zaika O, Jensen V, O Neil R, et al. The renal TRPV4 channel is essential for adaptation to increased dietary potassium. Kidney Int. 2017;91:1398-1409 pubmed publisher
Kicinska A, Augustynek B, Kulawiak B, Jarmuszkiewicz W, Szewczyk A, Bednarczyk P. A large-conductance calcium-regulated K+ channel in human dermal fibroblast mitochondria. Biochem J. 2016;473:4457-4471 pubmed
Gorin M, Tsitoura C, Kahan A, Watznauer K, Drose D, Arts M, et al. Interdependent Conductances Drive Infraslow Intrinsic Rhythmogenesis in a Subset of Accessory Olfactory Bulb Projection Neurons. J Neurosci. 2016;36:3127-44 pubmed publisher
Mizutani H, Yamamura H, Muramatsu M, Hagihara Y, Suzuki Y, Imaizumi Y. Modulation of Ca2+ oscillation and melatonin secretion by BKCa channel activity in rat pinealocytes. Am J Physiol Cell Physiol. 2016;310:C740-7 pubmed publisher

image

image 1 :

Western blot analysis of rat brain membranes: - 1. Anti-sloβ4 (KCNMB4) Antibody (#APC-061), (1:200).2. Anti- sloβ4 (KCNMB4) Antibody, preincubated with sloβ4/KCNMB4 Blocking Peptide (#BLP-PC061).

image 2 :

Expression of KCNMB4 in rat hippocampus and cortex - Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-sloβ4 (KCNMB4) Antibody (#APC-061), (1:200), followed by goat-anti-rabbit-AlexaFluor-488. A. KCNMB4 staining (green) in rat hippocampal CA1 region is detected in apical dendrites (arrows) of pyramidal neurons from the pyramidal layer (PL). B. Staining in rat parietal cortex shows staining of apical dendrites (arrows) of pyramidal neurons. Cell nuclei are stained with DAPI (blue).

image 3 :

Western blot analysisof rat brain membranes: - 1.Anti-slo?4 (KCNMB4) Antibody (#APC-061)(1:200).2. Anti-slo?4 (KCNMB4) Antibody preincubated with the negative control antigen.

product information

CAT :

APC-061

SKU :

APC-061-CF_0.2 ml

Product Name :

Anti-sloβ4 (KCNMB4) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q9ESK8

Applications :

IC IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PC061

Homology :

Mouse, human - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, N-terminal domain

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

AKLRVSYEYTEAEDKS(C), corresponding to amino acid residues 2-17 of rat KCNMB4

Product Page - Scientific background :

sloβ4 is a member of a family of regulatory β subunits that control the activity of the large conductance Ca2+-activated K+ channel, KCa1.1. The family includes four members with a shared topology: two transmembrane domains, short intracellular N- and C-termini and a large extracellular region.The four members of the family have a distinct tissue distribution with sloβ4 expressed almost exclusively in the central nervous system (CNS).Functionally, sloβ4 increases the sensitivity of the pore-forming KCa1.1 subunit to Ca2+ and voltage and it also changes its pharmacology. It has been shown that co-expression of sloβ4 with KCa1.1 makes the latter resistant to nanomolar concentrations of the well-known inhibitors charybdotoxin and iberiotoxin.The physiological significance of sloβ4 expression in the CNS is not clear, but KCa1.1 channels are likely involved in the regulation of neurotransmitter release in presynaptic terminals.

Applications may also work in :

IC IF IHC WB

Supplier :

Alomone Labs

Target :

Ca2+-activated K+ channel subunit β4, BKCa β subunit 4, BK beta4, Maxi K channel β4, Charybdotoxin receptor subunit beta-4

Short Description :

A Rabbit Polyclonal Antibody to slo?4 (KCNMB4) Subunit

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat sloβ4. Anti-sloβ4 (KCNMB4) Antibody (#APC-061) can be used in western blot, immunocytochemistry, and immunohistochemistry applications. It was designed to recognize sloβ4 from human, rat, and mouse samples.

Negative Control :

BLP-PC061

Positive Control :

Synonyms :

Ca2+-activated K+ channel subunit β4, BKCa β subunit 4, BK beta4, Maxi K channel β4, Charybdotoxin receptor subunit beta-4

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

KCNMB4

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:200

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage