Anti-CNGA3 Antibody | Alomone Labs APC-060 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-CNGA3 Antibody

catalog :

APC-060

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 2

Reference
Sakurai K, Vinberg F, Wang T, Chen J, Kefalov V. The Na(+)/Ca(2+), K(+) exchanger 2 modulates mammalian cone phototransduction. Sci Rep. 2016;6:32521 pubmed publisher
Bumbalo R, Lieber M, Schroeder L, Polat Y, Breer H, Fleischer J. Grueneberg Glomeruli in the Olfactory Bulb are Activated by Odorants and Cool Temperature. Cell Mol Neurobiol. 2017;37:729-742 pubmed publisher

image

image 1 :

Western blot analysis of rat brain membranes: - 1. Anti-CNGA3 Antibody (#APC-060), (1:200). 2. Anti-CNGA3 Antibody, preincubated with CNGA3 Blocking Peptide (#BLP-PC060).

image 2 :

Expression of CNG3 in rat brain - Immunohistochemical staining of frozen rat hippocampal dentate gyrus (DG) sections using Anti-CNGA3 Antibody (#APC-060). Neurons were found in the hilus of the DG with dendritic processes extending across the granule cell layer (arrows) both in the dorsal blade (A) and ventral blade (B). Diaminobenzidine color product is blue-black (arrows). DAPI is used as the counterstain (silver).

image 3 :

Expression of CNG3 in rat brain - Immunohistochemical staining of frozen rat hippocampal dentate gyrus (DG) sections usingAnti-CNGA3Antibody (#APC-060).Neurons were found in the hilus of the DG with dendritic processes extendingacross the granule cell layer (arrows) both in the dorsal blade (A) and ventral blade (B).Diaminobenzidine color product is blue-black (arrows).DAPI is used as the counterstain (silver).

product information

CAT :

APC-060

SKU :

APC-060-CF_0.2 ml

Product Name :

Anti-CNGA3 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q9QWN7

Applications :

IF IHC WB

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PC060

Homology :

Mouse - 15/18 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)GHGFSPDRENSEDASKAD, corresponding to amino acid residues 594-611 of rat CNGA3

Product Page - Scientific background :

Cyclic nucleotides are important second messengers in many cellular functions such as visual transduction, and relaxation of smooth muscle cells. Cyclic nucleotides exert their cellular functions through three major classes of cellular receptors, one of them is the cyclic nucleotide-gated (CNG) channels.1 The CNG channels are non-selective cation channels facilitating the influx of Na+ and Ca2+ ions, following activation by intracellular cyclic nucleotides.2 In vertebrates, six members of the CNG channel family were identified and grouped according to sequence homology into two subtypes, CNGA and CNGB. The native CNG channels are composed of a and b subunits in a tetrameric configuration. To date, four types of the a subunits (CNGA1-4) and two b subunits (CNGB1, CNGB3) have been characterized.3-4 Each subunit contains 6 TM domains and intracellular cAMP or cGMP binding domains but, are also modulated by other factors including phosphorylation and calmodulin.5 In a heterologous expression system, only the a subunits are capable of forming functional homomeric channels. CNG ion channels are essential in visual and olfactory signal transduction. The CNGA3 channel is primarily expressed in cone photoreceptors where it participates in light transduction (daylight and color vision). CNG channels expression was also found in the hippocampus, cortex, Purkinje cells and other neural derived tissues. CNGA3 was also detected in kidney, heart, brain and sperm, where it was thought to have an important role in the control of sperm motility and fertility. However, CNGA3-deficient mice were shown to be as fertile and produce at normal rate as wild types.6

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

Cyclic nucleotide-gated cation channel alpha 3, CNG channel alpha 3, CNG3, CNCG3, CNGα2, Cone photoreceptor cGMP-gated channel subunit alpha, CCNC1, ACHM2

Short Description :

A Rabbit Polyclonal Antibody to CNGA3 Channel

Long Description :

Anti-CNGA3 Antibody (#APC-060) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize CNGA3 from rat and mouse samples.

Negative Control :

BLP-PC060

Positive Control :

Synonyms :

Cyclic nucleotide-gated cation channel alpha 3, CNG channel alpha 3, CNG3, CNCG3, CNGα2, Cone photoreceptor cGMP-gated channel subunit alpha, CCNC1, ACHM2

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

CNGA3

AB Specifictiy :

Recognizes the two published isoforms of CNGA3.

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

ICC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage