Anti-KCNJ15 (Kir4.2) Antibody | Alomone Labs APC-058 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-KCNJ15 (Kir4.2) Antibody

catalog :

APC-058

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 2

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; mouse; 1:1000; loading ...	Poli G, Hasan S, Belia S, Cenciarini M, Tucker S, Imbrici P, et al. Kcnj16 (Kir5.1) Gene Ablation Causes Subfertility and Increases the Prevalence of Morphologically Abnormal Spermatozoa. Int J Mol Sci. 2021;22: pubmed publisher
	Lu J, Azad M, Moreau J, Zhu Y, Jiang X, Tonta M, et al. Inwardly rectifying potassium channels mediate polymyxin-induced nephrotoxicity. Cell Mol Life Sci. 2022;79:296 pubmed publisher

image

image 1 :

Western blot analysis of rat kidney membranes: - 1. Anti-KCNJ15 (Kir4.2) Antibody (#APC-058), (1:200).2. Anti-KCNJ15 (Kir4.2) Antibody, preincubated with KCNJ15/Kir4.2 Blocking Peptide (#BLP-PC058).

product information

CAT :

APC-058

SKU :

APC-058-CF_0.2 ml

Product Name :

Anti-KCNJ15 (Kir4.2) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

O88932

Applications :

IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PC058

Homology :

Guinea pig - 19/20 amino acid residues identical; human - 18/20 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminal domain

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

(C)EKQKLEEQYRQEDQRERELR, corresponding to amino acid residues 347-366 of mouse KCNJ15

Product Page - Scientific background :

Kir4.2 is a member of the family of inward rectifying K+ channels. The family includes 15 members that are structurally and functionally different from the voltage-dependent K+ channels.The family's topology consists of two transmembrane domains that flank a single and highly conserved pore region with intracellular N- and C-termini. As is the case for the voltage-dependent K+ channels the functional unit for the Kir channels is composed of four subunit that can assembly as either homo or heteromers.Kir channels are characterized by a K+ efflux that is limited by depolarizing membrane potentials thus making them essential for controlling resting membrane potential and K+ homeostasis.Kir4.2 is a member of the Kir4 subfamily that includes one other member: Kir4.1. Kir4.2 can co-assemble with Kir4.1 but also with other Kir channels such as Kir1.1 and Kir5.1.2The Kir4 subfamily has been classified as weak rectifiers with intermediate conductance. Kir4.2 is expressed in liver, kidney, pancreas lung and testis. Its physiological function is not well understood but it has been suggested that it could be involved in the regulation of K+ efflux in epithelial cells such as hepatocytes or lung cells.1

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

ATP-sensitive inward rectifier potassium channel 15, Potassium channel inwardly rectifying subfamily J member 15, Kir1.3, IRKK

Short Description :

A Rabbit Polyclonal Antibody to KCNJ15 (Kir4.2) Channel

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of the mouse Kir4.2 channel. Anti-KCNJ15 (Kir4.2) Antibody (#APC-058) can be used in western blot and immunohistochemistry applications. It has been designed to recognize Kir4.2 from rat, mouse and human samples.

Negative Control :

BLP-PC058

Positive Control :

Synonyms :

ATP-sensitive inward rectifier potassium channel 15, Potassium channel inwardly rectifying subfamily J member 15, Kir1.3, IRKK

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

KCNJ15

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage