Anti-HCN3 Antibody | Alomone Labs APC-057 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-HCN3 Antibody

catalog :

APC-057

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 8

Reference
Mu L, Liu X, Yu H, Vickstrom C, Friedman V, Kelly T, et al. cAMP-mediated upregulation of HCN channels in VTA dopamine neurons promotes cocaine reinforcement. Mol Psychiatry. 2023;28:3930-3942 pubmed publisher
Schoepf C, Zeidler M, Spiecker L, Kern G, Lechner J, Kummer K, et al. Selected Ionotropic Receptors and Voltage-Gated Ion Channels: More Functional Competence for Human Induced Pluripotent Stem Cell (iPSC)-Derived Nociceptors. Brain Sci. 2020;10: pubmed publisher
Zhong L, Fan X, Shi Z, Fan Z, Luo J, Lin N, et al. Hyperpolarization-Activated Cyclic Nucleotide-Gated Ion (HCN) Channels Regulate PC12 Cell Differentiation Toward Sympathetic Neuron. Front Cell Neurosci. 2019;13:415 pubmed publisher
Leon Aparicio D, Salvador C, Aparicio Trejo O, Briones Herrera A, Pedraza Chaverri J, Vaca L, et al. Novel Potassium Channels in Kidney Mitochondria: The Hyperpolarization-Activated and Cyclic Nucleotide-Gated HCN Channels. Int J Mol Sci. 2019;20: pubmed publisher
Dini L, Del Lungo M, Resta F, Melchiorre M, Spinelli V, Di Cesare Mannelli L, et al. Selective Blockade of HCN1/HCN2 Channels as a Potential Pharmacological Strategy Against Pain. Front Pharmacol. 2018;9:1252 pubmed publisher
Zhuang Q, Li G, Li B, Zhang C, Zhang X, Xi K, et al. Regularizing firing patterns of rat subthalamic neurons ameliorates parkinsonian motor deficits. J Clin Invest. 2018;128:5413-5427 pubmed publisher
Liu D, Wang X, Chu W, Lu N, Han W, Du Y, et al. Chronic cervical radiculopathic pain is associated with increased excitability and hyperpolarization-activated current ( Ih) in large-diameter dorsal root ganglion neurons. Mol Pain. 2017;13:1744806917707127 pubmed publisher
Negrini D, Marcozzi C, Solari E, Bossi E, Cinquetti R, Reguzzoni M, et al. Hyperpolarization-activated cyclic nucleotide-gated channels in peripheral diaphragmatic lymphatics. Am J Physiol Heart Circ Physiol. 2016;311:H892-H903 pubmed publisher

image

image 1 :

Western blot analysis of HCN3 in rat brain lysate: - 1. Anti-HCN3 Antibody (#APC-057), (1:200).2. Anti-HCN3 Antibody, preincubated with HCN3 Blocking Peptide (#BLP-PC057).

image 2 :

Expression of HCN3 in rat dorsal root ganglia (DRG) - Immunohistochemical staining of frozen section of rat DRG using Anti-HCN3 Antibody (#APC-057), (1:50) followed by mouse anti-rabbit Alexa Fluor 555 (red). Staining is present in neuronal cell bodies. Hoechst 33342 (blue) is used as counterstain.

image 3 :

Western blot analysisof HCN3 in rat brain lysate: - 1.Anti-HCN3Antibody (#APC-057) (1:200).2. Anti-HCN3 Antibody preincubated with the control peptide antigen.

product information

CAT :

APC-057

SKU :

APC-057-CF_0.2 ml

Product Name :

Anti-HCN3 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q9JKA8

Applications :

IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PC057

Homology :

Human, mouse - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)QRATGDGSPRRKGSGSER corresponding to amino acid residues 727-744 of rat HCN3

Product Page - Scientific background :

Hyperpolarization-activated cation currents (Ih) appear in the heart and brain and play a crucial role in controlling electrical pacemaker activity, contributing to biological processes such as heartbeat, sleep-wake cycle and synaptic plasticity.1,2The Ih currents are generated by the Hyperpolarization-activated cyclic nucleotide-gated channel family (HCN), which comprises of four homologous members, named HCN1-4. Each HCN subunit consist of six transmembrane domains (TM), a pore region between TM5-TM6 and a binding domain to cyclic nucleotides (CNBD) in the cytoplasmic C-terminus.2The HCN subunits can form functional homomers and can also co-assemble into functionally heteromers.2The channels are closely related to each other and share homology of about 60%. However, they differ from each other in their cytoplasmic N- and the C-terminus. HCN1-4 mainly differ from each other with regard to their speed of activation and the extent by which they are modulated by cAMP. HCN1 is the fastest channel, followed by HCN2, HCN3 and HCN4.2,3The HCN3 channels are abundant in the brain where high expression of HCN3 mRNA was found in the hypothalamus.

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

K+/Na+ Hyperpolarization-activated cyclic nucleotide-gated channel 3, BCNG4, Hac3

Short Description :

A Rabbit Polyclonal Antibody to HCN3 Channel

Long Description :

Anti-HCN3 Antibody (#APC-057) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize HCN3 from human, rat, and mouse samples.

Negative Control :

BLP-PC057

Positive Control :

Synonyms :

K+/Na+ Hyperpolarization-activated cyclic nucleotide-gated channel 3, BCNG4, Hac3

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

HCN3

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IHC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:50

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage