Anti-HCN1 Antibody | Alomone Labs APC-056 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-HCN1 Antibody

catalog :

APC-056

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - frozen section

more info or order :

Alomone Labs product webpage

citations: 22

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - frozen section; mouse; loading ...; fig s1r	Orefice L, Mosko J, Morency D, Wells M, Tasnim A, Mozeika S, et al. Targeting Peripheral Somatosensory Neurons to Improve Tactile-Related Phenotypes in ASD Models. Cell. 2019;178:867-886.e24 pubmed publisher
immunohistochemistry; rat; 1:100; loading ...; fig 3f	Zhao Z, Zhang K, Liu X, Yan H, Ma X, Zhang S, et al. Involvement of HCN Channel in Muscarinic Inhibitory Action on Tonic Firing of Dorsolateral Striatal Cholinergic Interneurons. Front Cell Neurosci. 2016;10:71 pubmed publisher
	Cai W, Liu S, Li B, Zhang X. Presynaptic HCN channels constrain GABAergic synaptic transmission in pyramidal cells of the medial prefrontal cortex. Biol Open. 2022;11: pubmed publisher
	Lezmy J, Arancibia Carcamo I, Quintela López T, Sherman D, Brophy P, Attwell D. Astrocyte Ca2+-evoked ATP release regulates myelinated axon excitability and conduction speed. Science. 2021;374:eabh2858 pubmed publisher
	Schoepf C, Zeidler M, Spiecker L, Kern G, Lechner J, Kummer K, et al. Selected Ionotropic Receptors and Voltage-Gated Ion Channels: More Functional Competence for Human Induced Pluripotent Stem Cell (iPSC)-Derived Nociceptors. Brain Sci. 2020;10: pubmed publisher
	Madden J, Davis O, Boyle K, Iredale J, Browne T, Callister R, et al. Functional and Molecular Analysis of Proprioceptive Sensory Neuron Excitability in Mice. Front Mol Neurosci. 2020;13:36 pubmed publisher
	Zhong L, Fan X, Shi Z, Fan Z, Luo J, Lin N, et al. Hyperpolarization-Activated Cyclic Nucleotide-Gated Ion (HCN) Channels Regulate PC12 Cell Differentiation Toward Sympathetic Neuron. Front Cell Neurosci. 2019;13:415 pubmed publisher
	Leon Aparicio D, Salvador C, Aparicio Trejo O, Briones Herrera A, Pedraza Chaverri J, Vaca L, et al. Novel Potassium Channels in Kidney Mitochondria: The Hyperpolarization-Activated and Cyclic Nucleotide-Gated HCN Channels. Int J Mol Sci. 2019;20: pubmed publisher
	Hammelmann V, Stieglitz M, Hülle H, Le Meur K, Kass J, Brümmer M, et al. Abolishing cAMP sensitivity in HCN2 pacemaker channels induces generalized seizures. JCI Insight. 2019;4: pubmed publisher
	Brown A, Arancillo M, Lin T, Catt D, Zhou J, Lackey E, et al. Molecular layer interneurons shape the spike activity of cerebellar Purkinje cells. Sci Rep. 2019;9:1742 pubmed publisher
	Dini L, Del Lungo M, Resta F, Melchiorre M, Spinelli V, Di Cesare Mannelli L, et al. Selective Blockade of HCN1/HCN2 Channels as a Potential Pharmacological Strategy Against Pain. Front Pharmacol. 2018;9:1252 pubmed publisher
	Nishitani A, Kunisawa N, Sugimura T, Sato K, Yoshida Y, Suzuki T, et al. Loss of HCN1 subunits causes absence epilepsy in rats. Brain Res. 2019;1706:209-217 pubmed publisher
	Zhuang Q, Li G, Li B, Zhang C, Zhang X, Xi K, et al. Regularizing firing patterns of rat subthalamic neurons ameliorates parkinsonian motor deficits. J Clin Invest. 2018;128:5413-5427 pubmed publisher
	Mader F, Muller S, Krause L, Springer A, Kernig K, Protzel C, et al. Hyperpolarization-Activated Cyclic Nucleotide-Gated Non-selective (HCN) Ion Channels Regulate Human and Murine Urinary Bladder Contractility. Front Physiol. 2018;9:753 pubmed publisher
	Silbernagel N, Walecki M, Schafer M, Kessler M, Zobeiri M, Rinné S, et al. The VAMP-associated protein VAPB is required for cardiac and neuronal pacemaker channel function. FASEB J. 2018;32:6159-6173 pubmed publisher
	Stieglitz M, Fenske S, Hammelmann V, Becirovic E, Schöttle V, Delorme J, et al. Disturbed Processing of Contextual Information in HCN3 Channel Deficient Mice. Front Mol Neurosci. 2017;10:436 pubmed publisher
	Resta F, Micheli L, Laurino A, Spinelli V, Mello T, Sartiani L, et al. Selective HCN1 block as a strategy to control oxaliplatin-induced neuropathy. Neuropharmacology. 2018;131:403-413 pubmed publisher
	Zobeiri M, Chaudhary R, Datunashvili M, Heuermann R, Lüttjohann A, Narayanan V, et al. Modulation of thalamocortical oscillations by TRIP8b, an auxiliary subunit for HCN channels. Brain Struct Funct. 2018;223:1537-1564 pubmed publisher
	Zhang K, Xu T, Yuan Z, Wei Z, Yamaki V, Huang M, et al. Essential roles of AMPA receptor GluA1 phosphorylation and presynaptic HCN channels in fast-acting antidepressant responses of ketamine. Sci Signal. 2016;9:ra123 pubmed
	Negrini D, Marcozzi C, Solari E, Bossi E, Cinquetti R, Reguzzoni M, et al. Hyperpolarization-activated cyclic nucleotide-gated channels in peripheral diaphragmatic lymphatics. Am J Physiol Heart Circ Physiol. 2016;311:H892-H903 pubmed publisher
	Matsumoto Makidono Y, Nakayama H, Yamasaki M, Miyazaki T, Kobayashi K, Watanabe M, et al. Ionic Basis for Membrane Potential Resonance in Neurons of the Inferior Olive. Cell Rep. 2016;16:994-1004 pubmed publisher
	Li Q, Cui P, Miao Y, Gao F, Li X, Qian W, et al. Activation of group I metabotropic glutamate receptors regulates the excitability of rat retinal ganglion cells by suppressing Kir and I h. Brain Struct Funct. 2017;222:813-830 pubmed publisher

image

image 1 :

Western blot analysis of rat brain membrane: - 1. Anti-HCN1 Antibody (#APC-056), (1:200).2. Anti-HCN1 Antibody, preincubated with HCN1 Blocking Peptide (#BLP-PC056).

image 2 :

Expression of HCN1 in mouse cerebellum - Immunohistochemical staining mouse cerebellum using Anti-HCN1 Antibody (#APC-056). A. HCN1 (green) appears in the cerebellar pinceau. B. Calcium binding calbindinD28-K (red), a marker of Purkinje neurons, is stained in the same section. C. Merge of the images demonstrates the position of the HCN1-positive pinceau structures at the axon initial segment of Purkinje neurons.

image 3 :

Expression of HCN1 in rat DRG primary culture - Immunocytochemical staining ofparaformaldehyde-fixed and permeabilized rat DRG primary culture. Cells were stained withAnti-HCN1Antibody (#APC-056) (1:300) followed by goat anti-rabbit-AlexaFluor-488 secondary antibody (green). Nuclear staining of cells using the cell-permeable dye Hoechst 33342 (blue).

product information

CAT :

APC-056

SKU :

APC-056-CF_0.2 ml

Product Name :

Anti-HCN1 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q9JKB0

Applications :

IC IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PC056

Homology :

Mouse - 18/19 amino acid residues identical; human - 16/19 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)KPNSASNSRDDGNSVYPSK, corresponding to amino acid residues 6-24 of rat HCN1

Product Page - Scientific background :

Hyperpolarization-activated cation currents (Ih) appear in the heart and the brain and have a crucial role in controlling electrical pacemaker activity, contributing to biological processes such as heartbeat, sleep-wake cycle and synaptic plasticity.1,2The Ih currents are generated by the hyperpolarization-activated cyclic nucleotide-gated channel family (HCN), which is comprised of four homologous members, HCN1-4.Each HCN subunit consists of six transmembrane domains (TM), a pore region between TM5-TM6 and a binding domain for cyclic nucleotides (CNBD) in the cytoplasmic C-terminus.2 The HCN subunits can form functional homomers and can also co-assemble into functional heteromers.2The channels are closely related to each other and share a homology of about 60%. However, their similarity decreases in the cytoplasmic N- and C-termini. The channels HCN1-4 mainly differ from each other in their speed of activation and the extent to which they are modulated by cAMP. HCN1, weakly affected by cAMP, is the fastest channel, followed by HCN2, HCN3 and HCN4. HCN1 is extensively expressed in the brain, in specific areas like the neocortex, hippocampus, cerebellum and superior colliculus.2,3

Applications may also work in :

IC IF IHC WB

Supplier :

Alomone Labs

Target :

Hyperpolarization-activated cyclic nucleotide-gated potassium channel 1, HAC2, Brain cyclic nucleotide-gated channel 1, BCNG1, Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1

Short Description :

A Rabbit Polyclonal Antibody to HCN1 Channel

Long Description :

Anti-HCN1 Antibody (#APC-056) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry and immunocytochemistry applications. It has been designed to recognize HCN1 from human, rat, and mouse samples.

Negative Control :

BLP-PC056

Positive Control :

Synonyms :

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

HCN1

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

KO-Validated :

yes

Cited Application :

IHC ICC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage