Guinea pig Anti-HCN1 Antibody | Alomone Labs APC-056-GP product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Guinea pig Anti-HCN1 Antibody

catalog :

APC-056-GP

clonality :

polyclonal

host :

guinea-pigs

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 5

Reference
Luque M, Schrott Fischer A, Dudas J, Pechriggl E, Brenner E, Rask Andersen H, et al. HCN channels in the mammalian cochlea: Expression pattern, subcellular location, and age-dependent changes. J Neurosci Res. 2021;99:699-728 pubmed publisher
McClatchy D, Yu N, Martinez Bartolome S, Patel R, Pelletier A, Lavalle Adam M, et al. Structural Analysis of Hippocampal Kinase Signal Transduction. ACS Chem Neurosci. 2018;9:3072-3085 pubmed publisher
Resta F, Micheli L, Laurino A, Spinelli V, Mello T, Sartiani L, et al. Selective HCN1 block as a strategy to control oxaliplatin-induced neuropathy. Neuropharmacology. 2018;131:403-413 pubmed publisher
Zobeiri M, Chaudhary R, Datunashvili M, Heuermann R, Lüttjohann A, Narayanan V, et al. Modulation of thalamocortical oscillations by TRIP8b, an auxiliary subunit for HCN channels. Brain Struct Funct. 2018;223:1537-1564 pubmed publisher
Gandla J, Lomada S, Lu J, Kuner R, Bali K. miR-34c-5p functions as pronociceptive microRNA in cancer pain by targeting Cav2.3 containing calcium channels. Pain. 2017;158:1765-1779 pubmed publisher

image

image 1 :

Western blot analysis of rat brain lysate: - 1. Guinea pig Anti-HCN1 Antibody (#APC-056-GP), (1:500).2. Guinea pig Anti-HCN1 Antibody, preincubated with HCN1 Blocking Peptide (#BLP-PC056).

image 2 :

Expression of HCN1 in rat cerebellum - Immunohistochemical staining of rat cerebellum using Guinea pig Anti-HCN1 Antibody (#APC-056-GP). A. HCN1 staining (red) appears in the pinceau structures (arrows). B. DAPI is used as the counterstain (blue). C. Merged image of A and B.

product information

CAT :

APC-056-GP

SKU :

APC-056-GP-CF_0.2 ml

Product Name :

Guinea pig Anti-HCN1 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q9JKB0

Applications :

IF IHC WB

Reactivity :

Mouse

Host :

Guinea Pig

Blocking Peptide :

BLP-PC056

Homology :

Mouse - 18/19 amino acid residues identical; human - 16/19 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Guinea pig total IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)KPNSASNSRDDGNSVYPSK, corresponding to amino acid residues 6-24 of rat HCN1

Product Page - Scientific background :

Hyperpolarization-activated cation currents (Ih) appear in the heart and the brain and have a crucial role in controlling electrical pacemaker activity, contributing to biological processes such as heartbeat, sleep-wake cycle and synaptic plasticity.1,2The Ih currents are generated by the hyperpolarization-activated cyclic nucleotide-gated channel family (HCN), which is comprised of four homologous members, HCN1-4.Each HCN subunit consists of six transmembrane domains (TM), a pore region between TM5-TM6 and a binding domain for cyclic nucleotides (CNBD) in the cytoplasmic C-terminus.2 The HCN subunits can form functional homomers and can also co-assemble into functional heteromers.2The channels are closely related to each other and share a homology of about 60%. However, their similarity decreases in the cytoplasmic N- and C-termini. The channels HCN1-4 mainly differ from each other in their speed of activation and the extent to which they are modulated by cAMP. HCN1, weakly affected by cAMP, is the fastest channel, followed by HCN2, HCN3 and HCN4. HCN1 is extensively expressed in the brain, in specific areas like the neocortex, hippocampus, cerebellum and superior colliculus.2,3

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

Hyperpolarization-activated cyclic nucleotide-gated potassium channel 1, HAC2, Brain cyclic nucleotide-gated channel 1, BCNG1, Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1

Short Description :

A Guinea Pig Polyclonal Antibody to HCN1 Channel

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of the rat HCN1 channel. Guinea pig Anti-HCN1 Antibody (#APC-056-GP, formerly AGP-203) raised in guinea pig can be used in western blot and immunohistochemistry applications. It has been designed to recognize HCN1 from human, rat and mouse samples. The antigen used to immunize guinea pigs is the same as Anti-HCN1 Antibody (#APC-056) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation, etc.

Negative Control :

BLP-PC056

Positive Control :

Synonyms :

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

HCN1

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

ICC IHC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:500

more info or order :

Alomone Labs product webpage