Anti-KCNK10 (TREK-2) Antibody | Alomone Labs APC-055 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-KCNK10 (TREK-2) Antibody

catalog :

APC-055

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 9

Reference
Paschou M, Maier L, Papazafiri P, Selescu T, Dedos S, Babes A, et al. Neuronal microRNAs modulate TREK two-pore domain K+ channel expression and current density. RNA Biol. 2020;17:651-662 pubmed publisher
Kanda H, Ling J, Tonomura S, Noguchi K, Matalon S, Gu J. TREK-1 and TRAAK Are Principal K+ Channels at the Nodes of Ranvier for Rapid Action Potential Conduction on Mammalian Myelinated Afferent Nerves. Neuron. 2019;104:960-971.e7 pubmed publisher
Zhang X, Xu Z, Shi K, Guo D, Li H, Wang L, et al. Elevated expression of TREK-TRAAK K2P channels in the retina of adult rd1 mice. Int J Ophthalmol. 2019;12:924-929 pubmed publisher
Fischer R, Roux A, Wareham L, Sappington R. Pressure-dependent modulation of inward-rectifying K+ channels: implications for cation homeostasis and K+ dynamics in glaucoma. Am J Physiol Cell Physiol. 2019;317:C375-C389 pubmed publisher
Viatchenko Karpinski V, Ling J, Gu J. Characterization of temperature-sensitive leak K+ currents and expression of TRAAK, TREK-1, and TREK2 channels in dorsal root ganglion neurons of rats. Mol Brain. 2018;11:40 pubmed publisher
Kim E, Lee D, Hong S, Han J, Kang D. Activation of TREK-1, but Not TREK-2, Channel by Mood Stabilizers. Int J Mol Sci. 2017;18: pubmed publisher
Mura C, Delgado R, Delgado M, Restrepo D, Bacigalupo J. A CLCA regulatory protein present in the chemosensory cilia of olfactory sensory neurons induces a Ca2+-activated Cl- current when transfected into HEK293. BMC Neurosci. 2017;18:61 pubmed publisher
Chen G, Kim Y, Li H, Luo H, Liu D, Zhang Z, et al. PD-L1 inhibits acute and chronic pain by suppressing nociceptive neuron activity via PD-1. Nat Neurosci. 2017;20:917-926 pubmed publisher
Liu Z, Wang F, Fischer G, Hogan Q, Yu H. Peripheral nerve injury induces loss of nociceptive neuron-specific G?i-interacting protein in neuropathic pain rat. Mol Pain. 2016;12: pubmed publisher

image

image 1 :

Western blot analysis of rat cerebellum lysate: - 1. Anti-KCNK10 (TREK-2) Antibody (#APC-055), (1:200). 2. Anti-KCNK10 (TREK-2) Antibody, preincubated with KCNK10/TREK-2 Blocking Peptide (#BLP-PC055).

image 2 :

Western blot analysisof rat cerebellum lysate: - 1.Anti-KCNK10 (TREK-2)Antibody (#APC-055)(1:200). 2. Anti-KCNK10 (TREK-2) Antibody preincubated with the control peptide antigen.

image 3 :

Immuno-colocalization of Slitrk1 and KV2.1 in rat parietal cortex - Immunohistochemical staining of immersion-fixed free floating rat brain frozen sections using rabbitAnti-Slitrk1 (extracellular) Antibody (#ASR-081) (1:200) andGuinea pig Anti-KV2.1 Antibody(#AGP-109) (1:200). A. Slitrk1 staining (red) appears in profiles of pyramidal neurons. B. KV2.1 staining (green) is detected in profiles of pyramidal neurons. C. Merge of the two images shows colocalization in several neurons (arrows). Cell nuclei are stained with DAPI (blue).

product information

CAT :

APC-055

SKU :

APC-055-CF_0.2 ml

Product Name :

Anti-KCNK10 (TREK-2) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q9JIS4

Applications :

IC IF IHC WB

Reactivity :

Human Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PC055

Homology :

Human - 19/20 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminal domain

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

TFRNYSLDEEKKEDETEKMC, corresponding to amino acid residues 475-494 of rat KCNK10

Product Page - Scientific background :

KCNK10 (TREK-2, K2P10.1) is a member of the 2-pore (2P) domain K+ channels. These K+ channels are also known as background or "leak" channels and help to set the resting membrane potential.K2P channels can be activated by a wide variety of stimuli including temperature, pH, mechanical stretch, inhalation anesthetics, etc. The channels can also be subclassified based on their specific activators. KCNK10 can be integrated to a K2P subfamily which includes K2P2.1 (TREK1) and K2P4.1 (TRAAK) that are activated by intracellular unsaturated fatty acids such as arachidonic acid, low intracellular pH and mechanical stretch. KCNK10 mRNA expression has been detected in the brain (primarily in the cerebellum) and in peripheral tissues such as pancreas and kidney.

Applications may also work in :

IC IF IHC WB

Supplier :

Alomone Labs

Target :

Potassium channel subfamily K member 10, K2P10.1, TWIK-related K+ channel 2

Short Description :

A Rabbit Polyclonal Antibody to KCNK10 Channel

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat KCNK10. Anti-KCNK10 (TREK-2) Antibody (#APC-055) can be used in western blot, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize KCNK10 channel from mouse, rat, and human samples.

Negative Control :

BLP-PC055

Positive Control :

Synonyms :

Potassium channel subfamily K member 10, K2P10.1, TWIK-related K+ channel 2

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

KCNK10

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IHC ICC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage