Anti-KCNE2 (MiRP1) Antibody | Alomone Labs APC-054 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-KCNE2 (MiRP1) Antibody

catalog :

APC-054

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

Alomone Labs product webpage

citations: 2

Reference
Lindner M, Gilhooley M, Palumaa T, Morton A, Hughes S, Hankins M. Expression and Localization of Kcne2 in the Vertebrate Retina. Invest Ophthalmol Vis Sci. 2020;61:33 pubmed publisher
Larimore J, Zlatic S, Arnold M, Singleton K, Cross R, Rudolph H, et al. Dysbindin Deficiency Modifies the Expression of GABA Neuron and Ion Permeation Transcripts in the Developing Hippocampus. Front Genet. 2017;8:28 pubmed publisher

image

image 1 :

Western blot analysis of rat heart membranes: - 1. Anti-KCNE2 (MiRP1) Antibody (#APC-054), (1:200).2. Anti-KCNE2 (MiRP1) Antibody, preincubated with KCNE2/MiRP1 Blocking Peptide (#BLP-PC054).

image 2 :

Expression of KCNE2 in mouse heart - Immunohistochemical staining of mouse heart frozen sections using Anti-KCNE2 (MiRP1) Antibody (#APC-054), (red). Immunoreactivity appears as a striated pattern in heart muscle.

image 3 :

Expression of KCNE2 in mouse heart - Immunohistochemical staining of mouse heart frozen sectionsusingAnti-KCNE2 (MiRP1)Antibody (#APC-054) (red). Immunoreactivity appearsas a striated pattern in heart muscle.

product information

CAT :

APC-054

SKU :

APC-054-CF_0.2 ml

Product Name :

Anti-KCNE2 (MiRP1) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P63161

Applications :

IC IF IHC IP WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PC054

Homology :

Mouse - 18/20 amino acid residues identical; human - 16/20 amino acid residues identical; rabbit - 15/20 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminal domain

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)IVEDWQQKYRSQILHLEDSK, corresponding to amino acid residues 88-107 of rat KCNE2

Product Page - Scientific background :

KCNE2 (or MiRP1) is a member of a family of proteins that regulate the activity of voltage-dependent K+ channels. Other members of the family are KCNE1 (IsK, MiNK), KCNE3 (MiRP2), KCNE4 (MiRP3) and KCNE5 (MiRP4).KCNE1 is the founding member of the family and KCNE2 was discovered based on its homology with KCNE1.1,2The KCNE regulatory subunits are small proteins (14-20 kD) with a type-1 integral membrane topology. It is believed that both the cytoplasmic C-terminus tail and the transmembrane domain are necessary for the interaction with the a subunits. The stoichiometry of the KCNE subunits with their partner a subunits in the native channels is not clear and ratios ranging from 2 to 14 KCNE subunits per 4 a subunits have been proposed.1,2KCNE2 is broadly distributed with prominent expression in the brain and heart, but also in other tissues such as skeletal muscle, pancreas and kidney.The best described functional association of KCNE2 is with the voltage-gated K+ channel KV11.1 (HERG). In the heart, complexes of the two proteins constitute the molecular underlay of the cardiac IKr current and mutations in the KCNE2 subunit are associated with cardiac arrhythmias.1 KCNE2 can form complexes with other channels including the K+ channels KV7.1 (KCNQ1) and KV4.3 and the hyperpolarization-activated cation channels HCN1 and HCN2. In the stomach KV7.1/KCNE2 complexes were found to be crucial for acid secretion as well as for the maintenance of structural integrity, and for parietal cell survival.3

Applications may also work in :

IC IF IHC IP WB

Supplier :

Alomone Labs

Target :

Potassium channel subunit β MiRP1, MinK-related peptide 1, Potassium voltage-gated channel subfamily E member 2

Short Description :

A Rabbit Polyclonal Antibody to KCNE2 (MiRP1) Subunit

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of human KCNE1. Anti-KCNE2 (MiRP1) Antibody (#APC-054) can be used in western blot, immunohistochemistry, immunocytochemistry and immunoprecipitation applications. It has been designed to recognize KCNE2 from rat, mouse and human samples.

Negative Control :

BLP-PC054

Positive Control :

Synonyms :

Potassium channel subunit β MiRP1, MinK-related peptide 1, Potassium voltage-gated channel subfamily E member 2

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

KCNE2

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage