Anti-HCN4 Antibody | Alomone Labs APC-052 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-HCN4 Antibody

catalog :

APC-052

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

shrews, human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - frozen section

more info or order :

Alomone Labs product webpage

citations: 46

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; shrews; 1:300; fig 2a	Zhong W, Darmani N. The HCN Channel Blocker ZD7288 Induces Emesis in the Least Shrew (Cryptotis parva). Front Pharmacol. 2021;12:647021 pubmed publisher
immunoprecipitation; rat; fig 4f immunocytochemistry; rat; 1:200; loading ...; fig 4h western blot; rat; 1:1000; fig 4d	Liu D, Song A, Qi X, van Vliet P, Xiao J, Xiong F, et al. Cohesin-protein Shugoshin-1 controls cardiac automaticity via HCN4 pacemaker channel. Nat Commun. 2021;12:2551 pubmed publisher
western blot; mouse; 1:250; fig 4b	Scherschel K, Bräuninger H, Mölders A, Erlenhardt N, Amin E, Jungen C, et al. Characterization of the HCN Interaction Partner TRIP8b/PEX5R in the Intracardiac Nervous System of TRIP8b-Deficient and Wild-Type Mice. Int J Mol Sci. 2021;22: pubmed publisher
immunohistochemistry - frozen section; mouse; 1:250; loading ...; fig 5b	Zanetti L, Kilicarslan I, Netzer M, Babai N, Seitter H, Koschak A. Function of cone and cone-related pathways in CaV1.4 IT mice. Sci Rep. 2021;11:2732 pubmed publisher
immunohistochemistry - frozen section; rat; 1:50; loading ...; fig 3a	Xiao Y, Cai X, Atkinson A, Logantha S, Boyett M, Dobrzynski H. Expression of connexin 43, ion channels and Ca2+-handling proteins in rat pulmonary vein cardiomyocytes. Exp Ther Med. 2016;12:3233-3241 pubmed
	Mu L, Liu X, Yu H, Vickstrom C, Friedman V, Kelly T, et al. cAMP-mediated upregulation of HCN channels in VTA dopamine neurons promotes cocaine reinforcement. Mol Psychiatry. 2023;28:3930-3942 pubmed publisher
	Nguyen L, Xu Y, Nair M, Bordey A. The mTOR pathway genes mTOR, Rheb, Depdc5, Pten, and Tsc1 have convergent and divergent impacts on cortical neuron development and function. bioRxiv. 2023;: pubmed publisher
	Bitay G, T xf3 th N, D xe9 ri S, Szlov xe1 k J, Kohajda Z, Varr xf3 A, et al. The Inhibition of the Small-Conductance Ca2+-Activated Potassium Channels Decreases the Sinus Node Pacemaking during Beta-Adrenergic Activation. Pharmaceuticals (Basel). 2022;15: pubmed publisher
	Nguyen H, Wu T, Needs D, Zhang H, Perelli R, DeLuca S, et al. Engineered bacterial voltage-gated sodium channel platform for cardiac gene therapy. Nat Commun. 2022;13:620 pubmed publisher
	Cai W, Liu S, Li B, Zhang X. Presynaptic HCN channels constrain GABAergic synaptic transmission in pyramidal cells of the medial prefrontal cortex. Biol Open. 2022;11: pubmed publisher
	Luque M, Glueckert R. Sequential Indirect Dual Immunohistochemistry with Primary Rabbit Antibodies on Cochlear Sections Using an Intermediate Heat-Denaturation Step. Curr Protoc. 2021;1:e239 pubmed publisher
	Liu X, Zhao R, Ding Q, Yao X, Tsang S. TRPC7 regulates the electrophysiological functions of embryonic stem cell-derived cardiomyocytes. Stem Cell Res Ther. 2021;12:262 pubmed publisher
	Schoepf C, Zeidler M, Spiecker L, Kern G, Lechner J, Kummer K, et al. Selected Ionotropic Receptors and Voltage-Gated Ion Channels: More Functional Competence for Human Induced Pluripotent Stem Cell (iPSC)-Derived Nociceptors. Brain Sci. 2020;10: pubmed publisher
	Xie D, Geng L, Xiong K, Zhao T, Wang S, Xue J, et al. Cold-Inducible RNA-Binding Protein Prevents an Excessive Heart Rate Response to Stress by Targeting Phosphodiesterase. Circ Res. 2020;126:1706-1720 pubmed publisher
	Grijalva S, Gu J, Li J, Fernandez N, Fan J, Sung J, et al. Engineered Cardiac Pacemaker Nodes Created by TBX18 Gene Transfer Overcome Source-Sink Mismatch. Adv Sci (Weinh). 2019;6:1901099 pubmed publisher
	Liang W, Han P, Kim E, Mak J, Zhang R, Torrente A, et al. Canonical Wnt signaling promotes pacemaker cell specification of cardiac mesodermal cells derived from mouse and human embryonic stem cells. Stem Cells. 2020;38:352-368 pubmed publisher
	Zhong L, Fan X, Shi Z, Fan Z, Luo J, Lin N, et al. Hyperpolarization-Activated Cyclic Nucleotide-Gated Ion (HCN) Channels Regulate PC12 Cell Differentiation Toward Sympathetic Neuron. Front Cell Neurosci. 2019;13:415 pubmed publisher
	Leon Aparicio D, Salvador C, Aparicio Trejo O, Briones Herrera A, Pedraza Chaverri J, Vaca L, et al. Novel Potassium Channels in Kidney Mitochondria: The Hyperpolarization-Activated and Cyclic Nucleotide-Gated HCN Channels. Int J Mol Sci. 2019;20: pubmed publisher
	Rajendran P, Challis R, Fowlkes C, Hanna P, Tompkins J, Jordan M, et al. Identification of peripheral neural circuits that regulate heart rate using optogenetic and viral vector strategies. Nat Commun. 2019;10:1944 pubmed publisher
	Samal E, Evangelista M, Galang G, Srivastava D, Zhao Y, Vedantham V. Premature MicroRNA-1 Expression Causes Hypoplasia of the Cardiac Ventricular Conduction System. Front Physiol. 2019;10:235 pubmed publisher
	Tomlinson L, Lu Z, Bentley R, Colley H, Murdoch C, Webb S, et al. Attenuation of doxorubicin-induced cardiotoxicity in a human in vitro cardiac model by the induction of the NRF-2 pathway. Biomed Pharmacother. 2019;112:108637 pubmed publisher
	Bhattacharyya S, Duan J, Wang L, Li B, Bhakta M, Fernandez Perez A, et al. Using Gjd3-CreEGFP mice to examine atrioventricular node morphology and composition. Sci Rep. 2019;9:2106 pubmed publisher
	Mondal A, Lackey J, Saeed M, Wu F, Johnson J, Huang C, et al. An Imaging Protocol to Discriminate Specialized Conduction Tissue During Congenital Heart Surgery. Semin Thorac Cardiovasc Surg. 2019;31:537-546 pubmed publisher
	Segal S, Kirschner Peretz N, Arbel Ganon L, Liang J, Li L, Marbach D, et al. Eliminating contraction during culture maintains global and local Ca2+ dynamics in cultured rabbit pacemaker cells. Cell Calcium. 2019;78:35-47 pubmed publisher
	Dini L, Del Lungo M, Resta F, Melchiorre M, Spinelli V, Di Cesare Mannelli L, et al. Selective Blockade of HCN1/HCN2 Channels as a Potential Pharmacological Strategy Against Pain. Front Pharmacol. 2018;9:1252 pubmed publisher
	Yu W, El Danaf R, Okawa H, Pacholec J, Matti U, Schwarz K, et al. Synaptic Convergence Patterns onto Retinal Ganglion Cells Are Preserved despite Topographic Variation in Pre- and Postsynaptic Territories. Cell Rep. 2018;25:2017-2026.e3 pubmed publisher
	Wang J, Yang Y, Li Y, Zhu J, Lian H, Shao X, et al. Long-term treatment with ivabradine in transgenic atrial fibrillation mice counteracts hyperpolarization-activated cyclic nucleotide gated channel overexpression. J Cardiovasc Electrophysiol. 2019;30:242-252 pubmed publisher
	Zhuang Q, Li G, Li B, Zhang C, Zhang X, Xi K, et al. Regularizing firing patterns of rat subthalamic neurons ameliorates parkinsonian motor deficits. J Clin Invest. 2018;128:5413-5427 pubmed publisher
	Silbernagel N, Walecki M, Schafer M, Kessler M, Zobeiri M, Rinné S, et al. The VAMP-associated protein VAPB is required for cardiac and neuronal pacemaker channel function. FASEB J. 2018;32:6159-6173 pubmed publisher
	Beier C, Palanker D, Sher A. Stereotyped Synaptic Connectivity Is Restored during Circuit Repair in the Adult Mammalian Retina. Curr Biol. 2018;28:1818-1824.e2 pubmed publisher
	Stieglitz M, Fenske S, Hammelmann V, Becirovic E, Schöttle V, Delorme J, et al. Disturbed Processing of Contextual Information in HCN3 Channel Deficient Mice. Front Mol Neurosci. 2017;10:436 pubmed publisher
	Kozasa Y, Nakashima N, Ito M, Ishikawa T, Kimoto H, Ushijima K, et al. HCN4 pacemaker channels attenuate the parasympathetic response and stabilize the spontaneous firing of the sinoatrial node. J Physiol. 2018;596:809-825 pubmed publisher
	Zobeiri M, Chaudhary R, Datunashvili M, Heuermann R, Lüttjohann A, Narayanan V, et al. Modulation of thalamocortical oscillations by TRIP8b, an auxiliary subunit for HCN channels. Brain Struct Funct. 2018;223:1537-1564 pubmed publisher
	Mitrofanova L, Gorshkov A, Konovalov P, Krylova J. Telocytes in the human sinoatrial node. J Cell Mol Med. 2018;22:521-532 pubmed publisher
	Yavari A, Bellahcene M, Bucchi A, Sirenko S, Pintér K, Herring N, et al. Mammalian γ2 AMPK regulates intrinsic heart rate. Nat Commun. 2017;8:1258 pubmed publisher
	Zhang M, Vollmer C, Nurse C. Adenosine and dopamine oppositely modulate a hyperpolarization-activated current Ih in chemosensory neurons of the rat carotid body in co-culture. J Physiol. 2018;596:3101-3117 pubmed publisher
	Sun Y, Timofeyev V, Dennis A, Bektik E, Wan X, Laurita K, et al. A Singular Role of IK1 Promoting the Development of Cardiac Automaticity during Cardiomyocyte Differentiation by IK1 -Induced Activation of Pacemaker Current. Stem Cell Rev. 2017;13:631-643 pubmed publisher
	Hulsmans M, Clauss S, Xiao L, Aguirre A, King K, Hanley A, et al. Macrophages Facilitate Electrical Conduction in the Heart. Cell. 2017;169:510-522.e20 pubmed publisher
	Lee M, Ha S, Park C, Park P, Fuchs R, Wei L, et al. Transcriptome of interstitial cells of Cajal reveals unique and selective gene signatures. PLoS ONE. 2017;12:e0176031 pubmed publisher
	Wang Y, Mesirca P, Marques Sule E, Zahradnikova A, Villejoubert O, D Ocón P, et al. RyR2R420Q catecholaminergic polymorphic ventricular tachycardia mutation induces bradycardia by disturbing the coupled clock pacemaker mechanism. JCI Insight. 2017;2: pubmed publisher
	Bhattacharyya S, Bhakta M, MUNSHI N. Phenotypically silent Cre recombination within the postnatal ventricular conduction system. PLoS ONE. 2017;12:e0174517 pubmed publisher
	Fujii M, Sakaguchi A, Kamata R, Nagao M, Kikuchi Y, Evans S, et al. Sfrp5 identifies murine cardiac progenitors for all myocardial structures except for the right ventricle. Nat Commun. 2017;8:14664 pubmed publisher
	Puller C, Arbogast P, Keeley P, Reese B, Haverkamp S. Dendritic stratification differs among retinal OFF bipolar cell types in the absence of rod photoreceptors. PLoS ONE. 2017;12:e0173455 pubmed publisher
	Park K, Randazzo G, Jones K, Brzezinski J. Gsg1, Trnp1, and Tmem215 Mark Subpopulations of Bipolar Interneurons in the Mouse Retina. Invest Ophthalmol Vis Sci. 2017;58:1137-1150 pubmed publisher
	Negrini D, Marcozzi C, Solari E, Bossi E, Cinquetti R, Reguzzoni M, et al. Hyperpolarization-activated cyclic nucleotide-gated channels in peripheral diaphragmatic lymphatics. Am J Physiol Heart Circ Physiol. 2016;311:H892-H903 pubmed publisher
	Li Q, Cui P, Miao Y, Gao F, Li X, Qian W, et al. Activation of group I metabotropic glutamate receptors regulates the excitability of rat retinal ganglion cells by suppressing Kir and I h. Brain Struct Funct. 2017;222:813-830 pubmed publisher

image

image 1 :

Western blot analysis of rat brain membranes: - 1. Anti-HCN4 Antibody (#APC-052), (1:200).2. Anti-HCN4 Antibody, preincubated with HCN4 Blocking Peptide (#BLP-PC052).

image 2 :

Expression of HCN4 in mouse thalamus - Immunohistochemical staining of mouse thalamus using Anti-HCN4 Antibody (#APC-052). A. HCN4 (red) appears in the neuropil of the lateral nucleus (LN). B. Staining of reticular nucleus (RN) with mouse anti-Parvalbumin. C. Confocal merge of HCN4 and Parvalbumin images demonstrates separate localization of these proteins in the thalamus.

image 3 :

GST fusion protein with the sequence HGHLHDSAEERRLIAEGDASPG EDRTPPGLAAEPERP corresponding to amino acid residues 119-155 of human HCN4 (AccessionQ9Y3Q4). Intracellular N-terminus.

product information

CAT :

APC-052

SKU :

APC-052-CF_0.2 ml

Product Name :

Anti-HCN4 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q9Y3Q4

Applications :

IC IF IHC IP WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PC052

Homology :

Rabbit - identical; rat - 35/37 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by DNA sequence and SDS-PAGE

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

GST fusion protein with the sequence HGHLHDSAEERRLIAEGDASPG EDRTPPGLAAEPERP, corresponding to amino acid residues 119-155 of human HCN4

Product Page - Scientific background :

Hyperpolarization-activated cation currents (Ih) appear in the heart and the brain and have a crucial role in controlling electrical pacemaker activity, contributing to biological processes such as heartbeat, sleep-wake cycle and synaptic plasticity.1,2The Ih currents are generated by the Hyperpolarization-activated cyclic nucleotide-gated channel family (HCN), which is comprised of four homologous members, HCN1-4.Each HCN subunit consists of six transmembrane domains (TM), a pore region between TM5-TM6 and a binding domain for cyclic nucleotides (CNBD) in the cytoplasmic C-terminus.2The HCN subunits can form functional homomers and can also co-assemble into functional heteromers.2The channels are closely related to each other and share a homology of about 60%. However, their similarity decreases in the cytoplasmic N- and C-termini. The HCN1-4 channels mainly differ from each other in their speed of activation and the extent to which they are modulated by cAMP. HCN1, weakly affected by cAMP, is the fastest channel, followed by HCN2, HCN3 and HCN4.HCN4 is highly expressed in a restricted manner in adult sinoatrial (SA) node, constituting a good molecular marker for the adult cardiac pacemaker and might serve as a unique marker of the developing SA node .4,5mRNA expression of HCN4 is most abundant in medial habenula and anterior and principal relay nuclei of the thalamus.6

Applications may also work in :

IC IF IHC IP WB

Supplier :

Alomone Labs

Target :

Hyperpolarization-activated cyclic nucleotide-gated potassium channel 4

Short Description :

A Rabbit Polyclonal Antibody to HCN4 Channel

Long Description :

Anti-HCN4 Antibody (#APC-052) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot, immunoprecipitation, immunocytochemistry, and immunohistochemistry applications. It has been designed to recognize HCN4 from human, rat, and mouse samples.

Negative Control :

BLP-PC052

Positive Control :

Synonyms :

Hyperpolarization-activated cyclic nucleotide-gated potassium channel 4

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

HCN4

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

The serum was depleted of anti-GST antibodies by affinity chromatography on immobilized GST and then the IgG fraction was purified on immobilized antigen.

UNSPSC :

41116161

KO-Validated :

yes

Cited Application :

IP IHC ICC Whole Tissue Staining

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

3 µg fusion protein per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage