Guinea pig Anti-HCN4 Antibody | Alomone Labs APC-052-GP product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Guinea pig Anti-HCN4 Antibody

catalog :

APC-052-GP

clonality :

polyclonal

host :

guinea-pigs

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 5

Reference
Louradour J, Bortolotti O, Torre E, Bidaud I, Lamb N, Fernandez A, et al. L-Type Cav1.3 Calcium Channels Are Required for Beta-Adrenergic Triggered Automaticity in Dormant Mouse Sinoatrial Pacemaker Cells. Cells. 2022;11: pubmed publisher
Zhong L, Fan X, Shi Z, Fan Z, Luo J, Lin N, et al. Hyperpolarization-Activated Cyclic Nucleotide-Gated Ion (HCN) Channels Regulate PC12 Cell Differentiation Toward Sympathetic Neuron. Front Cell Neurosci. 2019;13:415 pubmed publisher
Bhattacharyya S, Duan J, Wang L, Li B, Bhakta M, Fernandez Perez A, et al. Using Gjd3-CreEGFP mice to examine atrioventricular node morphology and composition. Sci Rep. 2019;9:2106 pubmed publisher
Mondal A, Lackey J, Saeed M, Wu F, Johnson J, Huang C, et al. An Imaging Protocol to Discriminate Specialized Conduction Tissue During Congenital Heart Surgery. Semin Thorac Cardiovasc Surg. 2019;31:537-546 pubmed publisher
Zobeiri M, Chaudhary R, Datunashvili M, Heuermann R, Lüttjohann A, Narayanan V, et al. Modulation of thalamocortical oscillations by TRIP8b, an auxiliary subunit for HCN channels. Brain Struct Funct. 2018;223:1537-1564 pubmed publisher

image

image 1 :

Western blot analysis of rat brain lysate: - 1. Guinea pig Anti-HCN4 Antibody (#APC-052-GP), (1:200).2. Guinea pig Anti-HCN4 Antibody, preincubated with HCN4 Blocking Peptide (#BLP-PC052).

image 2 :

Expression of HCN4 in mouse thalamus - Immunohistochemical staining of mouse thalamus using Guinea pig Anti HCN4 Antibody (#APC-052-GP). A. HCN4 staining (green) appears in the neuropil of the ventral posterior thalamic nucleus (VPL). B. Nuclear staining using DAPI as the counter stain (blue). C. Merged images of A and B.

product information

CAT :

APC-052-GP

SKU :

APC-052-GP-CF_0.2 ml

Product Name :

Guinea pig Anti-HCN4 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q9Y3Q4

Applications :

IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Guinea Pig

Blocking Peptide :

BLP-PC052

Homology :

Rabbit - identical; rat - 35/37 amino acid residues identical; mouse - 33/37 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Guinea pig total IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

GST fusion protein with the sequence HGHLHDSAEERRLIAEGDASPG EDRTPPGLAAEPERP, corresponding to amino acid residues 119-155 of human HCN4

Product Page - Scientific background :

Hyperpolarization-activated cation currents (Ih) appear in the heart and the brain and have a crucial role in controlling electrical pacemaker activity, contributing to biological processes such as heartbeat, sleep-wake cycle and synaptic plasticity.1,2The Ih currents are generated by the Hyperpolarization-activated cyclic nucleotide-gated channel family (HCN), which is comprised of four homologous members, HCN1-4.Each HCN subunit consists of six transmembrane domains (TM), a pore region between TM5-TM6 and a binding domain for cyclic nucleotides (CNBD) in the cytoplasmic C-terminus.2The HCN subunits can form functional homomers and can also co-assemble into functional heteromers.2The channels are closely related to each other and share a homology of about 60%. However, their similarity decreases in the cytoplasmic N- and C-termini. The HCN1-4 channels mainly differ from each other in their speed of activation and the extent to which they are modulated by cAMP. HCN1, weakly affected by cAMP, is the fastest channel, followed by HCN2, HCN3 and HCN4.HCN4 is highly expressed in a restricted manner in adult sinoatrial (SA) node, constituting a good molecular marker for the adult cardiac pacemaker and might serve as a unique marker of the developing SA node .4,5mRNA expression of HCN4 is most abundant in medial habenula and anterior and principal relay nuclei of the thalamus.6

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

Hyperpolarization-activated cyclic nucleotide-gated potassium channel 4

Short Description :

A Guinea Pig Polyclonal Antibody to HCN4 Channel

Long Description :

Guinea pig Anti-HCN4 Antibody (#APC-052-GP, formerly AGP-004), raised in guinea pigs, is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize HCN4 from human, rat and mouse samples. The antigen used to immunize guinea pigs is the same as Anti-HCN4 Antibody (#APC-052) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation, etc.

Negative Control :

BLP-PC052

Positive Control :

Synonyms :

Hyperpolarization-activated cyclic nucleotide-gated potassium channel 4

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

HCN4

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

The serum was depleted of anti-GST antibodies by affinity chromatography on immobilized GST and then the IgG fraction was purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

3 µg fusion protein per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage