Anti-KCNQ3 Antibody | Alomone Labs APC-051 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-KCNQ3 Antibody

catalog :

APC-051

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

Alomone Labs product webpage

citations: 15

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; mouse; loading ...; fig s5c	Jacko M, Weyn Vanhentenryck S, Smerdon J, Yan R, Feng H, Williams D, et al. Rbfox Splicing Factors Promote Neuronal Maturation and Axon Initial Segment Assembly. Neuron. 2018;97:853-868.e6 pubmed publisher
	Kr xfc ger J, Schubert J, Kegele J, Labalme A, Mao M, Heighway J, et al. Loss-of-function variants in the KCNQ5 gene are implicated in genetic generalized epilepsies. EBioMedicine. 2022;84:104244 pubmed publisher
	Sheng Z, Zhang H, Zheng P, Chen S, Gu Z, Zhou J, et al. Impaired Kv7 channel activity in the central amygdala contributes to elevated sympathetic outflow in hypertension. Cardiovasc Res. 2022;118:585-596 pubmed publisher
	Bayasgalan T, Stupniki S, Kovacs A, Csemer A, Szentesi P, Pocsai K, et al. Alteration of Mesopontine Cholinergic Function by the Lack of KCNQ4 Subunit. Front Cell Neurosci. 2021;15:707789 pubmed publisher
	Wang H, Hu S, Zhang S, Song Y, Wang X, Wang L, et al. KCNQ Channels in the Mesolimbic Reward Circuit Regulate Nociception in Chronic Pain in Mice. Neurosci Bull. 2021;37:597-610 pubmed publisher
	Verneuil J, Brocard C, Trouplin V, Villard L, Peyronnet Roux J, Brocard F. The M-current works in tandem with the persistent sodium current to set the speed of locomotion. PLoS Biol. 2020;18:e3000738 pubmed publisher
	Lauritano A, Moutton S, Longobardi E, Tran Mau Them F, Laudati G, Nappi P, et al. A novel homozygous KCNQ3 loss-of-function variant causes non-syndromic intellectual disability and neonatal-onset pharmacodependent epilepsy. Epilepsia Open. 2019;4:464-475 pubmed publisher
	Tirko N, Eyring K, Carcea I, Mitre M, Chao M, Froemke R, et al. Oxytocin Transforms Firing Mode of CA2 Hippocampal Neurons. Neuron. 2018;100:593-608.e3 pubmed publisher
	Yau M, Kim R, Wang C, Li J, Ammar T, Yang R, et al. One drug-sensitive subunit is sufficient for a near-maximal retigabine effect in KCNQ channels. J Gen Physiol. 2018;150:1421-1431 pubmed publisher
	Kim E, Zhang J, Pang W, Wang S, Lee K, Cavaretta J, et al. Reduced axonal surface expression and phosphoinositide sensitivity in Kv7 channels disrupts their function to inhibit neuronal excitability in Kcnq2 epileptic encephalopathy. Neurobiol Dis. 2018;118:76-93 pubmed publisher
	Paz R, Tubert C, Stahl A, Díaz A, Etchenique R, Murer M, et al. Inhibition of striatal cholinergic interneuron activity by the Kv7 opener retigabine and the nonsteroidal anti-inflammatory drug diclofenac. Neuropharmacology. 2018;137:309-321 pubmed publisher
	Ghezzi F, Monni L, Nistri A. Functional up-regulation of the M-current by retigabine contrasts hyperexcitability and excitotoxicity on rat hypoglossal motoneurons. J Physiol. 2018;596:2611-2629 pubmed publisher
	Parrilla Carrero J, Buchta W, Goswamee P, Culver O, McKendrick G, Harlan B, et al. Restoration of Kv7 Channel-Mediated Inhibition Reduces Cued-Reinstatement of Cocaine Seeking. J Neurosci. 2018;38:4212-4229 pubmed publisher
	Ambrosino P, Freri E, Castellotti B, Soldovieri M, Mosca I, Manocchio L, et al. Kv7.3 Compound Heterozygous Variants in Early Onset Encephalopathy Reveal Additive Contribution of C-Terminal Residues to PIP2-Dependent K+ Channel Gating. Mol Neurobiol. 2018;55:7009-7024 pubmed publisher
	Zhou J, Gao Y, Kosten T, Zhao Z, Li D. Acute stress diminishes M-current contributing to elevated activity of hypothalamic-pituitary-adrenal axis. Neuropharmacology. 2017;114:67-76 pubmed publisher

image

image 1 :

Western blot analysis of rat brain membranes: - 1. Anti-KCNQ3 Antibody (#APC-051), (1:200). 2. Anti-KCNQ3 Antibody, preincubated with KCNQ3 Blocking Peptide (#BLP-PC051).

image 2 :

Expression of KCNQ3 in rat hippocampus - Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-KCNQ3 Antibody (#APC-051), (1:200), followed by goat anti-rabbit-AlexaFluor-488. KCNQ3 immunoreactivity (green) appears in cells of the pyramidal layer (P, vertical arrows) and cells of the stratum radiatum (SR, horizontal arrows). Cell nuclei are stained with DAPI (blue).

product information

CAT :

APC-051

SKU :

APC-051-CF_0.2 ml

Product Name :

Anti-KCNQ3 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

O88944

Applications :

IC IF IHC IP WB

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PC051

Homology :

Mouse - identical; human - 18/19 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

AEGEKKEDNRYSDLKTIIC, corresponding to amino acid residues 668-686 of rat KCNQ3

Product Page - Scientific background :

The KCNQ family of voltage-gated K+ channels includes 5 known members: KCNQ1 to KCNQ5. Structurally, the KCNQ family belongs to the six transmembrane domain category of K+ channels. KCNQ family members can form either homomultimeric or heteromultimeric channels with different functional consequences. For example KCNQ2 and KCNQ3 heteromultimers give rise to a much larger channel current than when either protein is expressed alone, probably due to enhanced plasma membrane expression of the combined channel. Indeed, KCNQ2/KCNQ3 heteromultimers are believed to be the molecular correlates of the so-called M current. This current is a K+ neuronal current that is strongly inhibited by the activation of the M1 subtype of the muscarinic acetylcholine receptor. Mutations in either KCNQ2 or KCNQ3 are associated with a form of epilepsy known as benign familial neonatal convulsions (BFNC).

Applications may also work in :

IC IF IHC IP WB

Supplier :

Alomone Labs

Target :

KV7.3, Potassium voltage-gated channel subfamily KQT member 3, BFNC2

Short Description :

A Rabbit Polyclonal Antibody to KCNQ3 (KV7.3) Channel

Long Description :

Anti-KCNQ3 Antibody (#APC-051) is a highly specific antibody directed against an epitope of the rat KV7.3 channel. The antibody can be used in western blot, immunoprecipitation, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize KCNQ3 from rat, human, and mouse samples.

Negative Control :

BLP-PC051

Positive Control :

Synonyms :

KV7.3, Potassium voltage-gated channel subfamily KQT member 3, BFNC2

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

KCNQ3

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

KO-Validated :

yes

Cited Application :

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:200

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage