Anti-KCNQ2 Antibody | Alomone Labs APC-050 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-KCNQ2 Antibody

catalog :

APC-050

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 9

Reference
Bayasgalan T, Stupniki S, Kovacs A, Csemer A, Szentesi P, Pocsai K, et al. Alteration of Mesopontine Cholinergic Function by the Lack of KCNQ4 Subunit. Front Cell Neurosci. 2021;15:707789 pubmed publisher
Wang H, Hu S, Zhang S, Song Y, Wang X, Wang L, et al. KCNQ Channels in the Mesolimbic Reward Circuit Regulate Nociception in Chronic Pain in Mice. Neurosci Bull. 2021;37:597-610 pubmed publisher
Gou X, Hu T, Gou Y, Li C, Yi M, Jia M. Specific protein kinase C isoform exerts chronic inhibition on the slowly activating delayed-rectifier potassium current by affecting channel trafficking. Channels (Austin). 2021;15:262-272 pubmed publisher
Inagaki A, Hayashi M, Andharia N, Matsuda H. Involvement of butyrate in electrogenic K+ secretion in rat rectal colon. Pflugers Arch. 2019;471:313-327 pubmed publisher
Kim E, Zhang J, Pang W, Wang S, Lee K, Cavaretta J, et al. Reduced axonal surface expression and phosphoinositide sensitivity in Kv7 channels disrupts their function to inhibit neuronal excitability in Kcnq2 epileptic encephalopathy. Neurobiol Dis. 2018;118:76-93 pubmed publisher
Ghezzi F, Monni L, Nistri A. Functional up-regulation of the M-current by retigabine contrasts hyperexcitability and excitotoxicity on rat hypoglossal motoneurons. J Physiol. 2018;596:2611-2629 pubmed publisher
Parrilla Carrero J, Buchta W, Goswamee P, Culver O, McKendrick G, Harlan B, et al. Restoration of Kv7 Channel-Mediated Inhibition Reduces Cued-Reinstatement of Cocaine Seeking. J Neurosci. 2018;38:4212-4229 pubmed publisher
Valkova C, Liebmann L, Kramer A, Hübner C, Kaether C. The sorting receptor Rer1 controls Purkinje cell function via voltage gated sodium channels. Sci Rep. 2017;7:41248 pubmed publisher
Zhou J, Gao Y, Kosten T, Zhao Z, Li D. Acute stress diminishes M-current contributing to elevated activity of hypothalamic-pituitary-adrenal axis. Neuropharmacology. 2017;114:67-76 pubmed publisher

image

image 1 :

Western blot analysis of KCNQ2 transfected HEK-293 cells: - 1. Anti-KCNQ2 Antibody (#APC-050), (1:200). 2. Anti-KCNQ2 Antibody, preincubated with KCNQ2 Blocking Peptide (#BLP-PC050).

product information

CAT :

APC-050

SKU :

APC-050-CF_0.2 ml

Product Name :

Anti-KCNQ2 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

O88943

Applications :

IC IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PC050

Homology :

Mouse - identical; human - 15/16 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)RGPTITDKDRTKGPAE, corresponding to amino acid residues 578-593 of rat KCNQ2

Product Page - Scientific background :

The KCNQ family of voltage-gated K+ channels includes 5 known members: KCNQ1 to KCNQ5. Structurally, the KCNQ family belongs to the six transmembrane domain category of K+ channels. KCNQ family members can form either homo- or heteromultimeric channels with different functional consequences. For example KCNQ2 and KCNQ3 heteromultimers give rise to a much larger channel current than when either protein is expressed alone, probably due to enhanced plasma membrane expression of the combined channel. Indeed, KCNQ2/KCNQ3 heteromultimers are believed to be the molecular correlates of the so-called M current. This current is a K+ neuronal current that is strongly inhibited by the activation of the M1 subtype of the muscarinic acetylcholine receptor. Mutations in either KCNQ2 or KCNQ3 are associated with a form of epilepsy known as Benign familial neonatal convulsions (BNFC).

Applications may also work in :

IC IF IHC WB

Supplier :

Alomone Labs

Target :

KV7.2, Voltage-gated potassium channel QKT subfamily member 2, KvLQT2

Short Description :

A Rabbit Polyclonal Antibody to KCNQ2 (KV7.2) Channel

Long Description :

Anti-KCNQ2 Antibody (#APC-050) is a highly specific antibody directed against an epitope of the rat KV7.2 channel. The antibody can be used in western blot, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize KCNQ2 from rat, human, and mouse samples.

Negative Control :

BLP-PC050

Positive Control :

Synonyms :

KV7.2, Voltage-gated potassium channel QKT subfamily member 2, KvLQT2

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

KCNQ2

AB Specifictiy :

The antibody is specific for KCNQ2; it does not cross react with KCNQ3 or other QKT proteins. There are at least 9 recognized splice variants of rat KCNQ2. This antibody recognizes all except splice variants B and G.

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IP IHC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage