Guinea pig Anti-KCNQ2 Antibody | Alomone Labs APC-050-GP product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Guinea pig Anti-KCNQ2 Antibody

catalog :

APC-050-GP

clonality :

polyclonal

host :

guinea-pigs

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 2

Reference
Li S, Damonte V, Chen C, Wang G, Kebschull J, Yamaguchi H, et al. Hyperexcitable arousal circuits drive sleep instability during aging. Science. 2022;375:eabh3021 pubmed publisher
Zhang J, Kim E, Chen C, Procko E, Pant S, Lam K, et al. Identifying mutation hotspots reveals pathogenetic mechanisms of KCNQ2 epileptic encephalopathy. Sci Rep. 2020;10:4756 pubmed publisher

image

image 1 :

Western blot analysis of rat brain (lanes 1 and 2), mouse brain (lanes 3 and 4), human KCNQ2 transfected HEK-293 cells (lanes 5 and 7) and human brain neuroblastoma SH-SY5Y cell line (lanes 6 and 8) lysates: - 1,3,5,6. Guinea pig Anti-KCNQ2 Antibody (#APC-050-GP), (1:200).2,4,7,8. Guinea pig Anti-KCNQ2 Antibody, preincubated with KCNQ2 Blocking Peptide (#BLP-PC050).

image 2 :

Expression of KCNQ2 in rat brain - Immunohistochemical staining of free-floating frozen rat hippocampal CA1 region sections using Guinea pig Anti-KCNQ2 Antibody (#APC-050-GP), (1:200). A. KCNQ2 staining (red) appears in the pyramidal layer (P). B. Nuclei are stained using DAPI as the counterstain (blue).

product information

CAT :

APC-050-GP

SKU :

APC-050-GP-CF_0.2 ml

Product Name :

Guinea pig Anti-KCNQ2 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

O88943

Applications :

IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Guinea Pig

Blocking Peptide :

BLP-PC050

Homology :

Mouse - identical; human - 15/16 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Guinea pig total IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)RGPTITDKDRTKGPAE, corresponding to amino acid residues 578-593 of rat KCNQ2

Product Page - Scientific background :

The KCNQ family of voltage-gated K+ channels includes 5 known members: KCNQ1 to KCNQ5. Structurally, the KCNQ family belongs to the six transmembrane domain category of K+ channels.KCNQ family members can form either homo- or heteromultimeric channels with different functional outcomes. For example KCNQ2 and KCNQ3 heteromultimers give rise to a much larger channel current than when either protein is expressed alone, probably due to enhanced plasma membrane expression of the combined channel. Indeed, KCNQ2/KCNQ3 heteromultimers are believed to be the molecular correlates of the so-called M current. This current is a K+ neuronal current that is strongly inhibited by the activation of the M1 subtype of the muscarinic acetylcholine receptor.Mutations in either KCNQ2 or KCNQ3 are associated with a form of epilepsy known as Benign familial neonatal convulsions (BNFC).

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

KV7.2, Voltage-gated potassium channel QKT subfamily member 2, KvLQT2

Short Description :

A Guinea Pig Polyclonal Antibody to KCNQ2 (KV7.2) Channel

Long Description :

Guinea pig Anti-KCNQ2 Antibody (#APC-050-GP, formerly AGP-065), raised in guinea pigs, is a highly specific antibody directed against an epitope of the rat KV7.2 channel. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize KCNQ2 from mouse, rat, and human samples. The antigen used to immunize guinea pigs is the same as Anti-KCNQ2 Antibody (#APC-050) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation, etc.

Negative Control :

BLP-PC050

Positive Control :

Synonyms :

KV7.2, Voltage-gated potassium channel QKT subfamily member 2, KvLQT2

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

KCNQ2

AB Specifictiy :

The antibody is specific for KCNQ2. It does not cross-react with KCNQ3 or other QKT proteins. There are at least 9 recognized splice variants of rat KCNQ2. This antibody recognizes all except splice variants B and G.

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:200

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage