Anti-KCNK2 (TREK-1) Antibody | Alomone Labs APC-047 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-KCNK2 (TREK-1) Antibody

catalog :

APC-047

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 11

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; mouse; 1:200; loading ...; fig 6a	Bae Y, Choi J, Ryoo K, Kim A, Kwon O, Jung H, et al. Spadin Modulates Astrocytic Passive Conductance via Inhibition of TWIK-1/TREK-1 Heterodimeric Channels. Int J Mol Sci. 2020;21: pubmed publisher
	Kim A, Jung H, Kim Y, Kim S, Park J, Lee S, et al. The Knockdown of TREK-1 in Hippocampal Neurons Attenuate Lipopolysaccharide-Induced Depressive-Like Behavior in Mice. Int J Mol Sci. 2019;20: pubmed publisher
	Wang W, Kiyoshi C, Du Y, Taylor A, Sheehan E, Wu X, et al. TREK-1 Null Impairs Neuronal Excitability, Synaptic Plasticity, and Cognitive Function. Mol Neurobiol. 2020;57:1332-1346 pubmed publisher
	Kanda H, Ling J, Tonomura S, Noguchi K, Matalon S, Gu J. TREK-1 and TRAAK Are Principal K+ Channels at the Nodes of Ranvier for Rapid Action Potential Conduction on Mammalian Myelinated Afferent Nerves. Neuron. 2019;104:960-971.e7 pubmed publisher
	Zhang X, Xu Z, Shi K, Guo D, Li H, Wang L, et al. Elevated expression of TREK-TRAAK K2P channels in the retina of adult rd1 mice. Int J Ophthalmol. 2019;12:924-929 pubmed publisher
	Fang Y, Tian Y, Huang Q, Wan Y, Xu L, Wang W, et al. Deficiency of TREK-1 potassium channel exacerbates blood-brain barrier damage and neuroinflammation after intracerebral hemorrhage in mice. J Neuroinflammation. 2019;16:96 pubmed publisher
	Woo D, Bae J, Nam M, An H, Ju Y, Won J, et al. Activation of Astrocytic μ-opioid Receptor Elicits Fast Glutamate Release Through TREK-1-Containing K2P Channel in Hippocampal Astrocytes. Front Cell Neurosci. 2018;12:319 pubmed publisher
	Zhang J, Cao M, Chen Y, Wan Z, Wang H, Lin H, et al. Increased Expression of TREK-1 K+ Channel in the Dorsal Root Ganglion of Rats with Detrusor Overactivity After Partial Bladder Outlet Obstruction. Med Sci Monit. 2018;24:1064-1071 pubmed
	Kim E, Lee D, Hong S, Han J, Kang D. Activation of TREK-1, but Not TREK-2, Channel by Mood Stabilizers. Int J Mol Sci. 2017;18: pubmed publisher
	Cai Y, Peng Z, Guo H, Wang F, Zeng Y. TREK-1 pathway mediates isoflurane-induced memory impairment in middle-aged mice. Neurobiol Learn Mem. 2017;145:199-204 pubmed publisher
	Fang Y, Huang X, Wan Y, Tian H, Tian Y, Wang W, et al. Deficiency of TREK-1 potassium channel exacerbates secondary injury following spinal cord injury in mice. J Neurochem. 2017;141:236-246 pubmed publisher

image

image 1 :

Western blot analysis of rat brain membranes: - 1. Anti-KCNK2 (TREK-1) Antibody (#APC-047), (1:200).2. Anti-KCNK2 (TREK-1) Antibody, preincubated with KCNK2/TREK-1 Blocking Peptide (#BLP-PC047).

image 2 :

Peptide DPKSAAQNSKPRLSFSTK(C) corresponding to residues 8-25 of human KCNK2(AccessionO95069). Intracellular N-terminus.

image 3 :

Expression of TREK-1 in rat hippocampus. - Immunohistochemical staining of rat brain sections using Anti-KCNK2 (TREK-1) Antibody (#APC-047). TREK-1 staining (red) in the hippocampus is detected in the stratum radiatum in the neuropil. Similar diffused staining is observed for GLAST. GFAP staining the astrocyte marker is also shown.Adapted from Zhou M. et al. (2009) J. Neurosci. 29 8551. with permission of the Society for Neuroscience.

product information

CAT :

APC-047

SKU :

APC-047-CF_0.2 ml

Product Name :

Anti-KCNK2 (TREK-1) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

O95069

Applications :

IC IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PC047

Homology :

Rat - identical; mouse - 17/18 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

DPKSAAQNSKPRLSFSTK(C), corresponding to residues 8-25 of human KCNK2

Product Page - Scientific background :

KCNK2 (also named TWIK-related K+ channel, TREK-1 or K2P2.1) is a member of the 2-pore (2P) domain K+ channels family that includes at least 16 members. These channels show little time- or voltage-dependence and are considered to be "leak" or "background" K+ channels, thereby generating background currents which help set the membrane resting potential and cell excitation.The K2P channels have a signature topology that includes four transmembrane domains and two pore domains with intracellular N- and C- termini. K2P channels are regulated by diverse physical and chemical stimuli including temperature, changes in intracellular pH, mechanical stretch, inhalation anesthetics, etc. The channels can then be subclassified based in their specific activators. KCNK2 can be integrated to a K2P subfamily that includes K2P4.1 (TRAAK) and K2P10.1 (TREK2) that are activated by intracellular unsaturated fatty acids such as arachidonic acid, lysophosphatidic acid and mechanical stretch. In addition, KCNK2 can also be activated by general anesthetics such as halothane and chloroform and intracellular acidification.KCNK2 expression in humans is largely restricted to the brain with some expression in ovary and small intestine while KCNK2 expression in rodents is more widespread.KCNK2 has an important role in mood regulation, as knockout mice show resistance to depression, suggesting that KCNK2 may be a potential target for anti-depressants.KCNK2 is involved in the fast release of glutamate from astrocytes. It requires the activation of Gαi, dissociation of Gβγ, followed by the opening of the glutamate permeable KCNK2 (TREK-1) K+ channel through its interaction with Gβγ. For this purpose, KCNK2 is localized at the cell surface of the cell body and processes of astrocytes.

Applications may also work in :

IC IF IHC WB

Supplier :

Alomone Labs

Target :

Potassium channel subfamily K member 2, Outward rectifying potassium channel protein TREK-1, Twik-related K+ channel 1, K2P2.1, TPKC1

Short Description :

A Rabbit Polyclonal Antibody to KCNK2 Channel

Long Description :

Anti-KCNK2 (TREK-1) Antibody (#APC-047) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize TREK-1 from mouse, rat, and human samples.

Negative Control :

BLP-PC047

Positive Control :

Synonyms :

Potassium channel subfamily K member 2, Outward rectifying potassium channel protein TREK-1, Twik-related K+ channel 1, K2P2.1, TPKC1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

KCNK2

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

KO-Validated :

yes

Cited Application :

IP IHC ICC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage