Anti-CNGA2 Antibody | Alomone Labs APC-045 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-CNGA2 Antibody

catalog :

APC-045

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 11

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; mouse; 1:200	Inoue N, Nishizumi H, Ooyama R, Mogi K, Nishimori K, Kikusui T, et al. The olfactory critical period is determined by activity-dependent Sema7A/PlxnC1 signaling within glomeruli. elife. 2021;10: pubmed publisher
	Qu L, Wang Y, Ge S, Li N, Fu J, Zhang Y, et al. Altered Activity of SK Channel Underpins Morphine Withdrawal Relevant Psychiatric Deficiency in Infralimbic to Accumbens Shell Pathway. Front Psychiatry. 2019;10:240 pubmed publisher
	Del Bon F, Piazza C, Lancini D, Paderno A, Bosio P, Taboni S, et al. Open Partial Horizontal Laryngectomies for T3⁻T4 Laryngeal Cancer: Prognostic Impact of Anterior vs. Posterior Laryngeal Compartmentalization. Cancers (Basel). 2019;11: pubmed publisher
	Uytingco C, Williams C, Xie C, Shively D, Green W, Ukhanov K, et al. BBS4 is required for intraflagellar transport coordination and basal body number in mammalian olfactory cilia. J Cell Sci. 2019;132: pubmed publisher
	AlMatrouk A, Lemons K, Ogura T, Luo W, Wilson C, Lin W. Chemical Exposure-Induced Changes in the Expression of Neurotrophins and Their Receptors in the Main Olfactory System of Mice Lacking TRPM5-Expressing Microvillous Cells. Int J Mol Sci. 2018;19: pubmed publisher
	Pyrski M, Tusty M, Eckstein E, Oboti L, Rodriguez Gil D, Greer C, et al. P/Q Type Calcium Channel Cav2.1 Defines a Unique Subset of Glomeruli in the Mouse Olfactory Bulb. Front Cell Neurosci. 2018;12:295 pubmed publisher
	Maurya D, Bohm S, Alenius M. Hedgehog signaling regulates ciliary localization of mouse odorant receptors. Proc Natl Acad Sci U S A. 2017;114:E9386-E9394 pubmed publisher
	Villar P, Delgado R, Vergara C, Reyes J, Bacigalupo J. Energy Requirements of Odor Transduction in the Chemosensory Cilia of Olfactory Sensory Neurons Rely on Oxidative Phosphorylation and Glycolytic Processing of Extracellular Glucose. J Neurosci. 2017;37:5736-5743 pubmed publisher
	Williams C, Uytingco C, Green W, McIntyre J, Ukhanov K, Zimmerman A, et al. Gene Therapeutic Reversal of Peripheral Olfactory Impairment in Bardet-Biedl Syndrome. Mol Ther. 2017;25:904-916 pubmed publisher
	Bader A, Bintig W, Begandt D, Klett A, Siller I, Gregor C, et al. Adenosine receptors regulate gap junction coupling of the human cerebral microvascular endothelial cells hCMEC/D3 by Ca2+ influx through cyclic nucleotide-gated channels. J Physiol. 2017;595:2497-2517 pubmed publisher
	Ferguson C, Zhao H. Simultaneous Loss of NCKX4 and CNG Channel Desensitization Impairs Olfactory Sensitivity. J Neurosci. 2017;37:110-119 pubmed publisher

image

image 1 :

Western blot analysis of rat brain membranes: - 1. Anti-CNGA2 Antibody (#APC-045), (1:200).2. Anti-CNGA2 Antibody, preincubated with CNGA2 Blocking Peptide (#BLP-PC045).

image 2 :

Expression of CNGA2 in rat cerebellum - Immunohistochemical staining of rat cerebellum using Anti-CNGA2 Antibody (#APC-045). A. CNGA2 (red) appears in Purkinje cells (vertical arrows) and in astrocytic fibers (horizontal arrows) traversing the cerebellar molecular layer (Mol). B. Staining of astrocytes with mouse anti-glial fibrillary acidic protein (GFAP, green) demonstrates the full distribution of astrocytic fibers (horizontal arrows) in the cerebellum. C. Confocal merge of panels A and B.

image 3 :

Peptide (C)KQNHEDDYLSDGINTPEP corresponding to amino acid residues 643-660 of rat CNGA2. (AccessionQ00195). Intracellular C-terminus.

product information

CAT :

APC-045

SKU :

APC-045-CF_0.2 ml

Product Name :

Anti-CNGA2 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q00195

Applications :

IC IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PC045

Homology :

Mouse - identical; rabbit - 15/18 amino acid residues identical; human, bovine - 14/18 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)KQNHEDDYLSDGINTPEP, corresponding to amino acid residues 643-660 of rat CNGA2.

Product Page - Scientific background :

Cyclic nucleotides are important second messengers in many cellular functions such as visual transduction, and relaxation of smooth muscle cells. Cyclic nucleotides exert their cellular functions through three major classes of cellular receptors, one of them is the cyclic nucleotide-gated (CNG) channels.1 The CNG channels are non-selective cation channels facilitating the influx of Na+ and Ca2+ ions, following activation by intracellular cyclic nucleotides.2 In vertebrates, six members of the CNG channel family were identified and grouped according to sequence homology into two subtypes, CNGA and CNGB. To date, four types of the a subunits (CNGA1-4) and two b subunits (CNGB1, CNGB3) have been characterized.3 Native CNG channels are composed of a and b subunits in a tetrameric configuration. Each subunit contains 6 TM domains and intracellular cAMP or cGMP binding domains, but are also modulated by other factors including phosphorylation and calmodulin.4 In a heterologous expression system, only the a subunits are capable of forming functional homomeric channels. CNG ion channels are essential in visual and olfactory signal transduction.CNG channels were originally detected in rod and cone photoreceptors and olfactory receptor cells, where they mediate the transduction of external sensory stimuli into neuronal activity.5CNGA2 is predominantly expressed in olfactory neurons (the olfactory type receptor). However, electrophysiological and molecular data indicate that CNGA1, and especially CNGA2, are widely distributed and functionally active in many regions of the brain, including the hippocampus, cerebral cortex, cerebellum, and brainstem.6

Applications may also work in :

IC IF IHC WB

Supplier :

Alomone Labs

Target :

Cyclic nucleotide-gated cation channel 2, CNG channel alpha-2, CNG2, CNCG2, Olfactory cyclic nucleotide gated channel 1, OCNC1

Short Description :

A Rabbit Polyclonal Antibody to CNGA2 Channel

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an intracellular epitope of the rat CNGA2 channel. Anti-CNGA2 Antibody (#APC-045) can be used in western blot, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize CNGA2 from human, rat, and mouse samples.

Negative Control :

BLP-PC045

Positive Control :

Synonyms :

Cyclic nucleotide-gated cation channel 2, CNG channel alpha-2, CNG2, CNCG2, Olfactory cyclic nucleotide gated channel 1, OCNC1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

CNGA2

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IHC ICC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:100

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage