Anti-KCNK9 (TASK-3) (extracellular) Antibody | Alomone Labs APC-044 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-KCNK9 (TASK-3) (extracellular) Antibody

catalog :

APC-044

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 5

Published Application/Species/Sample/Dilution	Reference
western blot; human; 1:800; fig 4	Sun H, Luo L, Lal B, Ma X, Chen L, Hann C, et al. A monoclonal antibody against KCNK9 K(+) channel extracellular domain inhibits tumour growth and metastasis. Nat Commun. 2016;7:10339 pubmed publisher
	Rakoczy R, Schiebrel C, Wyatt C. Acute Oxygen-Sensing via Mitochondria-Generated Temperature Transients in Rat Carotid Body Type I Cells. Front Physiol. 2022;13:874039 pubmed publisher
	Garc a Morales V, Rodr guez Bey G, G mez P rez L, Dom nguez V as G, Gonz lez Forero D, Portillo F, et al. Sp1-regulated expression of p11 contributes to motor neuron degeneration by membrane insertion of TASK1. Nat Commun. 2019;10:3784 pubmed publisher
	Wang X, Guan R, Zhao X, Zhu D, Song N, Shen L. TASK1 and TASK3 Are Coexpressed With ASIC1 in the Ventrolateral Medulla and Contribute to Central Chemoreception in Rats. Front Cell Neurosci. 2018;12:285 pubmed publisher
	Yuan F, Wang H, Feng J, Wei Z, Yu H, Zhang X, et al. Leptin Signaling in the Carotid Body Regulates a Hypoxic Ventilatory Response Through Altering TASK Channel Expression. Front Physiol. 2018;9:249 pubmed publisher

image

image 1 :

Western blot analysis of KCNK9 in rat cerebellum (lanes 1, 4), human mammary gland MCF-10A (lanes 2, 5) and MCF-7 (lanes 3, 6) cell lysates (upper panel): - 1-3. Anti-KCNK9 (TASK-3) (extracellular) Antibody (#APC-044).4-6. Anti-KCNK9 (TASK-3) (extracellular) Antibody, preincubated with KCNK9/TASK-3 (extracellular) Blocking Peptide (#BLP-PC044). Lower panel: anti-β actin to ensure equal loading. Note that the expected 50 kD MW band is present in cerebellum and in the tumorigenic breast cancer cell line (MCF-7) but not in the non-tumorigenic breast cell line (MCF-10A).

image 2 :

Western blot analysis of KCNK9 in rat cerebellum (lanes 1 4)human mammary gland MCF-10A (lanes 2 5)and MCF-7 (lanes 3 6)cell lysates (upper panel): - 1-3.Anti-KCNK9 (TASK-3)(extracellular) Antibody (#APC-044).4-6. Anti-KCNK9 (TASK-3)(extracellular) Antibody preincubated with the control peptide antigen.Lower panel: anti-? actin to ensure equal loading.Note that the expected 50 kD MW band is present in cerebellum and in the tumorigenicbreast cancer cell line (MCF-7) but not in the non-tumorigenic breast cell line (MCF-10A).

image 3 :

Western blot analysisof rat cerebellum lysate: - 1.Anti-KCNK9 (TASK-3)(extracellular) Antibody (#APC-044)(1:200).2. Anti-KCNK9 (TASK-3)(extracellular) Antibody preincubated with thecontrol peptide antigen.

product information

CAT :

APC-044

SKU :

APC-044-CF_0.2 ml

Product Name :

Anti-KCNK9 (TASK-3) (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q9ES08

Applications :

IF IHC WB

Reactivity :

Human Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PC044

Homology :

Human - 15/17 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, near the P1 loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)DDYQQLELVILQSEPHR, corresponding to amino acid residues 57-73 of rat KCNK9

Product Page - Scientific background :

KCNK9 (also named TASK3 or K2P9.1) is a member of the 2-pore (2P) domain K+ channels family that probably assemble as dimers to constitute a functional channel.1 These channels show little time- or voltage-dependence and are considered to be "leak" or "background" K+ channels, thereby generating background currents which help set the membrane resting potential and cell excitation. The channels are regulated by diverse physical and chemical stimuli including temperature, pH, mechanical stretch, inhalation anesthetics, etc.2 The physiological role of the K2P channels is not yet clear, though they were proposed to participate in breathing, aldosterone secretion and anesthetic-mediated neuronal activity.In human, expression of KCNK9 is restricted in brain and its expression overlaps with the structurally related KCNK3 (TASK1, K2P3.1) channel. In rat, it is more widely expressed in other tissues as well as in brain. Unexpectedly, KCNK9 was found to be over-expressed between 5 to 100-fold in 44% of breast cancer tumors tested. Transfection and over-expression of KCNK9 in cell lines promotes tumor growth and confers resistance to hypoxia and serum deprivation.3 Recently, KCNK9 was found to play an important role in K+-dependent apoptosis of cerebellar granules.4

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

Potassium channel subfamily K member 9, K2P9.1, TWIK-related acid-sensitive K+ channel 3, Two pore K+ channel KT3.2

Short Description :

A Rabbit Polyclonal Antibody to KCNK9 Channel

Long Description :

Anti-KCNK9 (TASK-3) (extracellular) Antibody (#APC-044) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. The antibody recognizes an extracellular epitope and is thus ideal for detecting the channel in living cells. It has been designed to recognize TASK-3 from rat and human samples.

Negative Control :

BLP-PC044

Positive Control :

Synonyms :

Potassium channel subfamily K member 9, K2P9.1, TWIK-related acid-sensitive K+ channel 3, Two pore K+ channel KT3.2

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

KCNK9

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

ICC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage