Anti-Kir2.2 (KCNJ12) Antibody | Alomone Labs APC-042 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Kir2.2 (KCNJ12) Antibody

catalog :

APC-042

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat kidney (lanes 1 and 3) and brain (lanes 2 and 4) membranes: - 1,2. Anti-Kir2.2 (KCNJ12) Antibody (#APC-042), (1:200). 3,4. Anti-Kir2.2 (KCNJ12) Antibody, preincubated with Kir2.2/KCNJ12 Blocking Peptide (#BLP-PC042).

image 2 :

Expression of Kir2.2 in rat cerebellum. - Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-Kir2.2 (KCNJ12) Antibody (#APC-042), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. Kir2.2 immunoreactivity (green) appeared in Bergmann glial fibers (right-pointing arrows) and outlines of molecular layer neurons (left-pointing arrows). B. Pre-incubation of the antibody with Kir2.2/KCNJ12 Blocking Peptide (BLP-PC042), suppressed staining. Cell nuclei are stained with DAPI (blue). MOL = molecular layer.

image 3 :

Western blot analysis of rat brain (lanes 1 and 4) mouse brain (lanes 2 and 5) and rat stomach (lanes 3 and 6) lysates: - 1-3. Anti-xCT/SLC7A11 (extracellular) Antibody(#ANT-111) (1:800).4-6. Anti-xCT/SLC7A11 (extracellular) Antibody preincubated with the negative control antigen.

product information

CAT :

APC-042

SKU :

APC-042-CF_0.2 ml

Product Name :

Anti-Kir2.2 (KCNJ12) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P52188

Applications :

IC IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PC042

Homology :

Mouse identical or 16/19 amino acid residues identical; human 13/19 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminal domain

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)EVATDRDGRSPQPEHDFDR, corresponding to amino acid residues 391-409 of rat Kir2.2

Product Page - Scientific background :

Kir2.2 is a member of the inward rectifying K+ channel family. The Kir2 subfamily comprises 4 known members: Kir2.1, Kir2.2, Kir2.3 and Kir2.4. The Kir2 subfamily members are strong inward rectifiers and are believed to be involved in maintaining the resting membrane potential and buffering of extracellular K+.The Kir2.2 subunit is expressed in the CNS, kidney and heart where it has been found to form heteromeric channels with Kir2.1.

Applications may also work in :

IC IF IHC WB

Supplier :

Alomone Labs

Target :

Inward rectifier K+ channel Kir2.2, IRK-2, IRK2, ATP-sensitive inward rectifier potassium channel 12

Short Description :

A Rabbit Polyclonal Antibody to Kir2.2 (KCNJ12) Channel

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of the rat IRK-2 channel. Anti-Kir2.2 (KCNJ12) Antibody (#APC-042) can be used in western blot, and immunocytochemistry applications. It has been designed to recognize IRK-2 from human, rat and mouse samples.

Negative Control :

BLP-PC042

Positive Control :

Synonyms :

Inward rectifier K+ channel Kir2.2, IRK-2, IRK2, ATP-sensitive inward rectifier potassium channel 12

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

KCNJ12

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:300

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage