Anti-KCNK6 (TWIK-2) Antibody | Alomone Labs APC-040 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-KCNK6 (TWIK-2) Antibody

catalog :

APC-040

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse

application :

western blot

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat brain membranes: - 1. Anti-KCNK6 (TWIK-2) Antibody (#APC-040), (1:200).2. Anti-KCNK6 (TWIK-2) Antibody, preincubated with KCNK6/TWIK-2 Blocking Peptide (#BLP-PC040).

image 2 :

Western blot analysisof rat brain membranes: - 1.Anti-KCNK6 (TWIK-2) Antibody (#APC-040) (1:200).2. Anti-KCNK6(TWIK-2) Antibody preincubated with the control peptide antigen.

image 3 :

Western blot analysis of human brain glioblastoma cell line (U-87 MG) lysate: - 1. Anti-xCT/SLC7A11 (extracellular) Antibody(1:200).2. Anti-xCT/SLC7A11 (extracellular) Antibody preincubated with the negative control antigen.

product information

CAT :

APC-040

SKU :

APC-040-CF_0.2 ml

Product Name :

Anti-KCNK6 (TWIK-2) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q9Y257

Applications :

Reactivity :

Human Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PC040

Homology :

Rat - 13/17 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

(C)ESHQQLSASSHTDYASIPR, corresponding to residues 295-313 of human KCNK6

Product Page - Scientific background :

KCNK6 (also named Tandem of P domain of Weak Inward rectifier K+ channel 2, TWIK-2 or K2P6.1) is a member of the 2-pore (2P) domain K+ channel family that includes at least 16 members. These channels show little time- or voltage-dependence and are considered to be "leak" or "background" K+ channels, thereby generating background currents which help set the membrane resting potential and cell excitation.The K2P channels have a signature topology that includes four transmembrane domains and two pore domains with intracellular N- and C- termini. The functional channel is believed to be a dimer.K2P channels are regulated by diverse physical and chemical stimuli including temperature, changes in intracellular pH, mechanical stretch, inhalation anesthetics, etc. The channels can then be subclassified based in their specific activators.KCNK6 displays low single channel conductance of about 5pS and is partially activated by arachidonic acid and blocked by volatile anesthetics.KCNK6 expression is widespread, especially in the rat were it is expressed in brain, heart, kidney and liver. In humans KCNK6 expression is more restricted with expression in aorta, stomach, placenta and spleen while in mouse, KCNK6 expression has been detected only in liver.

Applications may also work in :

Supplier :

Alomone Labs

Target :

Potassium channel subfamily K member 6, TOSS, K2P6.1

Short Description :

A Rabbit Polyclonal Antibody to KCNK6 Channel

Long Description :

Anti-KCNK6 (TWIK-2) Antibody (#APC-040) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot analysis. It has been designed to recognize KCNK6 from rat and human samples.

Negative Control :

BLP-PC040

Positive Control :

Synonyms :

Potassium channel subfamily K member 6, TOSS, K2P6.1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

KCNK6

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage