Anti-KCNN1 (KCa2.1, SK1) Antibody | Alomone Labs APC-039 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-KCNN1 (KCa2.1, SK1) Antibody

catalog :

APC-039

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - frozen section

more info or order :

Alomone Labs product webpage

citations: 10

Published Application/Species/Sample/Dilution	Reference
western blot; mouse; 1:500; loading ...; fig 6a	Deng P, Carlin D, Oh Y, Myrick L, Warren S, Cavalli V, et al. Voltage-Independent SK-Channel Dysfunction Causes Neuronal Hyperexcitability in the Hippocampus of Fmr1 Knock-Out Mice. J Neurosci. 2019;39:28-43 pubmed publisher
western blot; rat; loading ...; fig 3a	Kim T, Terentyeva R, Roder K, Li W, Liu M, Greener I, et al. SK channel enhancers attenuate Ca2+-dependent arrhythmia in hypertrophic hearts by regulating mito-ROS-dependent oxidation and activity of RyR. Cardiovasc Res. 2017;113:343-353 pubmed publisher
immunohistochemistry - frozen section; mouse; 1:200; fig 5 immunocytochemistry; mouse; 1:100; fig 3 western blot; mouse; 1:1000; fig 1	Li Y, Hu H, Butterworth M, Tian J, Zhu M, O Neil R. Expression of a Diverse Array of Ca2+-Activated K+ Channels (SK1/3, IK1, BK) that Functionally Couple to the Mechanosensitive TRPV4 Channel in the Collecting Duct System of Kidney. PLoS ONE. 2016;11:e0155006 pubmed publisher
	Rahm A, Wieder T, Gramlich D, Müller M, Wunsch M, El Tahry F, et al. Differential regulation of KCa 2.1 (KCNN1) K+ channel expression by histone deacetylases in atrial fibrillation with concomitant heart failure. Physiol Rep. 2021;9:e14835 pubmed publisher
	Mi Z, Yang J, He Q, Zhang X, Xiao Y, Shu Y. Alterations of Electrophysiological Properties and Ion Channel Expression in Prefrontal Cortex of a Mouse Model of Schizophrenia. Front Cell Neurosci. 2019;13:554 pubmed publisher
	Ling T, Yi F, Lu T, Wang X, Sun X, Willis M, et al. F-box protein-32 down-regulates small-conductance calcium-activated potassium channel 2 in diabetic mouse atria. J Biol Chem. 2019;294:4160-4168 pubmed publisher
	Fan X, Yu Y, Lan H, Ou X, Yang L, Li T, et al. Ca2+/Calmodulin-Dependent Protein Kinase II (CaMKII) Increases Small-Conductance Ca2+-Activated K+ Current in Patients with Chronic Atrial Fibrillation. Med Sci Monit. 2018;24:3011-3023 pubmed publisher
	Fu X, Pan Y, Cao Q, Li B, Wang S, Du H, et al. Metformin restores electrophysiology of small conductance calcium-activated potassium channels in the atrium of GK diabetic rats. BMC Cardiovasc Disord. 2018;18:63 pubmed publisher
	Lu T, Wang X, Chai Q, Sun X, Sieck G, Katusic Z, et al. Role of the endothelial caveolae microdomain in shear stress-mediated coronary vasorelaxation. J Biol Chem. 2017;292:19013-19023 pubmed publisher
	Torrente A, Zhang R, Wang H, Zaini A, Kim B, Yue X, et al. Contribution of small conductance K+ channels to sinoatrial node pacemaker activity: insights from atrial-specific Na+ /Ca2+ exchange knockout mice. J Physiol. 2017;595:3847-3865 pubmed publisher

image

image 1 :

Western blot analysis of rat brain cortex: - 1. Anti-KCNN1 (KCa2.1, SK1) Antibody (#APC-039), (1:200). 2. Anti-KCNN1 (KCa2.1, SK1) Antibody, preincubated with KCNN1/KCa2.1 Blocking Peptide (#BLP-PC039).

image 2 :

Expression of KCa2.1 in rat parietal cortex. - Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-KCNN1 (KCa2.1, SK1) Antibody (#APC-039), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. KCa2.1 immunoreactivity (green) appears in outlines of cortical neurons (arrows). B. Pre-incubation of the antibody with KCNN1 (KCa2.1, SK1) Blocking Peptide (BLP-PC039), suppressed staining. Cell nuclei are stained with DAPI (blue).

image 3 :

Peptide (C)THHFKDAFSGRDTS corresponding to amino acid residues 218-231 of mouse cystine/glutamate transporter (AccessionQ9WTR6). 3rdextracellular loop.

product information

CAT :

APC-039

SKU :

APC-039-CF_0.2 ml

Product Name :

Anti-KCNN1 (KCa2.1, SK1) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P70606

Applications :

IC IF IHC WB

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PC039

Homology :

Mouse - 17/19 amino acid residues identical; human - 12/19 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, N-terminal domain

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)DRPGSGKPPTVSHRLGHRR corresponding to amino acid residues 75-93 of rat KCNN1

Product Page - Scientific background :

KCa2.1 is a member of the Ca2+-activated K+ channel family with small conductance that includes KCa2.2 (SK2) and KCa2.3 (SK3). The channel is voltage insensitive and is activated by intracellular Ca2+ in the submicromolar range. It has a similar topology to that of voltage-dependent K+ channels (KV channels), that is, six transmembrane domains and intracellular N- and C-termini. KCa2.1 channel expression is roughly confined to neuronal tissue, although there are some indications that it may be present in non-neuronal tissues as well. The sensitivity of KCa2.1 to the bee venom toxin Apamin (#STA-200) is a matter of some controversy. Expression of the human KCa2.1 in mammalian cell lines showed that most cells produce channels that can be blocked by Apamin. However, expression of KCa2.1 in Xenopus oocytes produced channels that were mostly insensitive to Apamin. Interestingly, the rat KCa2.1 is unable to form functional channels when expressed alone in either cell lines or oocytes.

Applications may also work in :

IC IF IHC WB

Supplier :

Alomone Labs

Target :

SKCa1, Small conductance calcium-activated potassium channel protein 1

Short Description :

A Rabbit Polyclonal Antibody to KCNN1 (KCa2.1, SK1) Channel

Long Description :

Anti-KCNN1 (KCa2.1, SK1) Antibody (#APC-039) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunocytochemistry, and immunohistochemistry applications. It has been designed to recognize KCNN1 from human, rat, and mouse samples.

Negative Control :

BLP-PC039

Positive Control :

Synonyms :

SKCa1, Small conductance calcium-activated potassium channel protein 1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

KCNN1

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IP IHC ICC Electrophysiology

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:200

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage