Anti-GIRK3 (Kir3.3) Antibody | Alomone Labs APC-038 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-GIRK3 (Kir3.3) Antibody

catalog :

APC-038

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 2

Reference
Tabak G, Keren Raifman T, Kahanovitch U, Dascal N. Mutual action by Gγ and Gβ for optimal activation of GIRK channels in a channel subunit-specific manner. Sci Rep. 2019;9:508 pubmed publisher
Booker S, Althof D, Gross A, Loreth D, MÃ¼ller J, Unger A, et al. KCTD12 Auxiliary Proteins Modulate Kinetics of GABAB Receptor-Mediated Inhibition in Cholecystokinin-Containing Interneurons. Cereb Cortex. 2017;27:2318-2334 pubmed publisher

image

image 1 :

Western blot analysis of rat brain membranes: - 1. Anti-GIRK3 (Kir3.3) Antibody (#APC-038), (1:200).2. Anti-GIRK3 (Kir3.3) Antibody, preincubated with GIRK3/Kir3.3 Blocking Peptide (#BLP-PC038).

image 2 :

Expression of GIRK3 in mouse deep cerebellar nuclei (DCN). - Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-GIRK3 (Kir3.3) Antibody (#APC-038), (1:400), followed by goat anti-rabbit-Alexa-488. A. GIRK3 immunoreactivity (green) appears in DCN cells (arrows). B. Pre-incubation of the antibody with GIRK3 (Kir3.3) Blocking Peptide (#BLP-PC038), suppressed staining. Cell nuclei are stained with DAPI (blue).

image 3 :

Alomone Labs NAX-5055 activates GalR2 receptor-mediated Ca2+mobilization in CHO-K1-G?16-mt-Aequorin cells. - Dose response of normalizedNAX-5055(#N-360) activation of GalR2 receptor-mediated Ca2+mobilization measured as aequorin-derived fluorescence following NAX-5055 application (EC50= 6.12 nM).

product information

CAT :

APC-038

SKU :

APC-038-CF_0.2 ml

Product Name :

Anti-GIRK3 (Kir3.3) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q63511

Applications :

IC IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PC038

Homology :

Mouse, human - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)RLDAHLYWSIPSRLDEKV, corresponding to residues 344-361 of rat KCNJ9

Product Page - Scientific background :

Kir3.3 (or G-protein regulated Inward-Rectifier K+ channel, GIRK3) is a member of the family of inward rectifying K+ channels. The family includes 15 members that are structurally and functionally different from the voltage-dependent K+ channels.The family's topology consists of two transmembrane domains that flank a single and highly conserved pore region with intracellular N- and C-termini. As is the case for the voltage-dependent K+ channels the functional unit for the Kir channels is composed of four subunit that can assembly as either homo or heterotetramers.Kir channels are characterized by a K+ efflux that is limited by depolarizing membrane potentials thus making them essential for controlling resting membrane potential and K+ homeostasis.Kir3.3 is a member of the Kir3.x subfamily that includes four members (Kir3.1- Kir3.4). The Kir3 family is characterized by the fact that the channels can be activated by neurotransmitters and other factors acting via the activation of G-protein coupled receptors. Binding of the corresponding ligand to the G-protein receptor induces the dissociation of Ga-GTP from the Gbg dimer. The latter directly binds to Kir3 and activates the channel.1,2Kir3.3 is mainly expressed in the brain, were it co-assembles with Kir3.1 or Kir3.2. The functional impact of Kir3.3 is less well understood than the other Kir3 channels. However, heteromers composed of Kir3.2 and Kir3.3 were found to be primarily responsible for the opioid-induced current and hyperpolarization observed in mouse locus ceruleus (LC) neurons.3

Applications may also work in :

IC IF IHC WB

Supplier :

Alomone Labs

Target :

G protein-activated inward rectifier potassium channel 3, KCNJ9

Short Description :

A Rabbit Polyclonal Antibody to GIRK3 (Kir3.3) Channel

Long Description :

Anti-GIRK3 (Kir3.3) Antibody (#APC-038) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize Kir3.3 from human, rat, and mouse samples.

Negative Control :

BLP-PC038

Positive Control :

Synonyms :

G protein-activated inward rectifier potassium channel 3, KCNJ9

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

KCNJ9

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

KO-Validated :

yes

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:400

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage