Anti-KCNK5 (TASK-2) Antibody | Alomone Labs APC-037 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-KCNK5 (TASK-2) Antibody

catalog :

APC-037

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Yuan F, Wang H, Feng J, Wei Z, Yu H, Zhang X, et al. Leptin Signaling in the Carotid Body Regulates a Hypoxic Ventilatory Response Through Altering TASK Channel Expression. Front Physiol. 2018;9:249 pubmed publisher

image

image 1 :

Western blot analysis of rat kidney membranes: - 1. Anti-KCNK5 (TASK-2) Antibody (#APC-037), (1:200).2. Anti-KCNK5 (TASK-2) Antibody, preincubated with KCNK5/TASK-2 Blocking Peptide (#BLP-PC037).

image 2 :

Expression of KCNK5 in rat pancreas - Immunohistochemical staining of rat pancreas was visualized using Anti-KCNK5 (TASK-2) Antibody (#APC-037), (1:50). Paraffin embedded sections of rat pancreas showing both endocrine (Islets of Langerhans) and exocrine areas. Strong and highly specific staining is evident only in endocrine cells (arrow). Universal Immuno-alkaline-phosphatase Polymer followed by New Fuchsin Substrate (Histofine, Nichirei Corp.) was used for the color reaction. Hematoxilin is used as the counterstain.

image 3 :

Expression of KCNK5 in rat pancreas - Immunohistochemical staining of rat pancreas was visualized usingAnti-KCNK5 (TASK-2) Antibody (#APC-037) (1:50). Paraffin embedded sections of rat pancreas showing both endocrine (Islets of Langerhans) and exocrine areas. Strong and highly specific staining is evident only in endocrine cells (arrow). Universal Immuno-alkaline-phosphatase Polymer followed by New Fuchsin Substrate (Histofine Nichirei Corp.) was used for the color reaction.Hematoxilin is used as the counterstain.

product information

CAT :

APC-037

SKU :

APC-037-CF_0.2 ml

Product Name :

Anti-KCNK5 (TASK-2) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

O95279

Applications :

IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PC037

Homology :

Mouse - 14/17 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

(C)YEQLMNEYNKANSPKGT, amino acid residues 483-499 of human KCNK5

Product Page - Scientific background :

KCNK5 (also named TWIK-related acid sensitive K+ channel 2, TASK-2 or K2P5.1) is a member of the 2-pore (2P) domain K+ channels family that includes at least 16 members. These channels show little time- or voltage-dependence and are considered to be "leak" or "background" K+ channels, thereby generating background currents which help set the membrane resting potential and cell excitation.The K2P channels have a signature topology that includes four transmembrane domains and two pore domains with intracellular N- and C- termini. The functional channel is believed to be a dimer.K2P channels are regulated by diverse physical and chemical stimuli including temperature, changes in intracellular pH, mechanical stretch, inhalation anesthetics, etc. The channels can then be subclassified based in their specific activators.As indicated by its name, KCNK5 (K2P5.1) is sensitive to variations in external pH. KCNK5 current is maximal at pH 8.8 but it is only 10% at pH 6.5. The channel is expressed in several epithelial tissues including pancreas, lung, small intestine and specially kidney. In the kidney KCNK5 has an important function in NaHCO3 absorption in the proximal tubule as knockout mice for KCNK5 display metabolic acidosis and hypotension.

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

Potassium channel subfamily K member 5, K2P5.1, TWIK-related acid sensitive K+ channel 2

Short Description :

A Rabbit Polyclonal Antibody to KCNK5 Channel

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of human KCNK5. Anti-KCNK5 (TASK-2) Antibody (#APC-037) can be used in western blot and immunohistochemistry applications. It has been designed to recognize KCNK5 from rat, mouse and human samples.

Negative Control :

BLP-PC037

Positive Control :

Synonyms :

Potassium channel subfamily K member 5, K2P5.1, TWIK-related acid sensitive K+ channel 2

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

KCNK5

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

ICC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:50

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage