Anti-sloβ1 (KCNMB1) Antibody | Alomone Labs APC-036 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-sloβ1 (KCNMB1) Antibody

catalog :

APC-036

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 8

Published Application/Species/Sample/Dilution	Reference
western blot; human; 1:200; loading ...; fig 7b	Kicinska A, Augustynek B, Kulawiak B, Jarmuszkiewicz W, Szewczyk A, Bednarczyk P. A large-conductance calcium-regulated K+ channel in human dermal fibroblast mitochondria. Biochem J. 2016;473:4457-4471 pubmed
	Salazar Enciso R, Guerrero Hern xe1 ndez A, G xf3 mez A, Benitah J, Rueda A. Aldosterone-Induced Sarco/Endoplasmic Reticulum Ca2+ Pump Upregulation Counterbalances Cav1.2-Mediated Ca2+ Influx in Mesenteric Arteries. Front Physiol. 2022;13:834220 pubmed publisher
	Wang L, Ling D, Huang M, Tao L, Tong Q, Hou Y, et al. Influence of atherosclerosis on the molecular expression of the TRPC1/BK signal complex in the aortic smooth muscles of mice. Exp Ther Med. 2022;23:4 pubmed publisher
	Noda S, Suzuki Y, Yamamura H, Giles W, Imaizumi Y. Roles of LRRC26 as an auxiliary γ1-subunit of large-conductance Ca2+-activated K+ channels in bronchial smooth muscle cells. Am J Physiol Lung Cell Mol Physiol. 2020;318:L366-L375 pubmed publisher
	Goff K, Goldberg E. Vasoactive intestinal peptide-expressing interneurons are impaired in a mouse model of Dravet syndrome. elife. 2019;8: pubmed publisher
	Balderas E, Torres N, Rosa Garrido M, Chaudhuri D, Toro L, Stefani E, et al. MitoBKCa channel is functionally associated with its regulatory β1 subunit in cardiac mitochondria. J Physiol. 2019;597:3817-3832 pubmed publisher
	Sun H, Liu Y, Song Z, She G, Xiao G, Wang Y, et al. Tyrphostin AG556 increases the activity of large conductance Ca2+ -activated K+ channels by inhibiting epidermal growth factor receptor tyrosine kinase. J Cell Mol Med. 2017;21:1826-1834 pubmed publisher
	Mizutani H, Yamamura H, Muramatsu M, Hagihara Y, Suzuki Y, Imaizumi Y. Modulation of Ca2+ oscillation and melatonin secretion by BKCa channel activity in rat pinealocytes. Am J Physiol Cell Physiol. 2016;310:C740-7 pubmed publisher

image

image 1 :

Western blot analysis of rat smooth muscle lysate: - 1. Anti-sloβ1 (KCNMB1) Antibody (#APC-036), (1:200).2. Anti-sloβ1 (KCNMB1) Antibody, preincubated with sloβ1/KCNMB1 Blocking Peptide (#BLP-PC036).

image 2 :

Peptide KKLVMAQKRGETRALC corresponding to amino acid residues 2-17 of ratslo?1 (AccessionP97678). Intracellular N-terminal part.

image 3 :

Western blot analysisof rat colon lysate: - 1.Anti-slo?1 (KCNMB1)Antibody (#APC-036) (1:400).2. Anti-slo?1 (KCNMB1) Antibody preincubated with the control peptide antigen.

product information

CAT :

APC-036

SKU :

APC-036-CF_0.2 ml

Product Name :

Anti-sloβ1 (KCNMB1) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P97678

Applications :

IC IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PC036

Homology :

Mouse, human, dog, rabbit - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, N-terminal domain

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

KKLVMAQKRGETRALC, corresponding to amino acid residues 2-17 of rat sloβ1

Product Page - Scientific background :

sloβ1 is a member of a family of regulatory β subunits that control the activity of the large conductance Ca2+-activated K+ channel KCa1.1. The family includes four members with a shared topology: two trans-membrane domains, short intracellular N- and C-termini and a large extracellular region. The four members of the family have a distinct tissue distribution with sloβ1 expressed almost exclusively in smooth muscle.Functionally, sloβ1 increases the sensitivity of the pore-forming KCa1.1 subunit to Ca2+ and voltage and it also changes its pharmacology.In the past few years there has been a lot of interest regarding the role of the sloβ1 subunit in the regulation of vascular tone and hypertension. Studies in sloβ1 knockout mice have shown that loss of this subunit results in systemic hypertension. Conversely, a recent study showed that a commonly found gain-of-function sloβ1 variant has a protective effect against human diastolic hypertension.

Applications may also work in :

IC IF IHC WB

Supplier :

Alomone Labs

Target :

Maxi potassium channel beta, MaxiKbeta, Ca2+-activated K+ channel subunit β1, BKCa subunit β1, BK beta1, Charybdotoxin receptor subunit beta-1, K(VCA)beta-1, Slowpoke-beta

Short Description :

A Rabbit Polyclonal Antibody to slo?1 (KCNMB1) Subunit

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat sloβ1. Anti-sloβ1 (KCNMB1) Antibody (#APC-036) can be used in western blot, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize sloβ1 from human, rat and mouse samples.

Negative Control :

BLP-PC036

Positive Control :

Synonyms :

Maxi potassium channel beta, MaxiKbeta, Ca2+-activated K+ channel subunit β1, BKCa subunit β1, BK beta1, Charybdotoxin receptor subunit beta-1, K(VCA)beta-1, Slowpoke-beta

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

KCNMB1

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IP IHC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:400

more info or order :

Alomone Labs product webpage