Guinea pig Anti-Kir4.1 (KCNJ10) Antibody | Alomone Labs APC-035-GP product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Guinea pig Anti-Kir4.1 (KCNJ10) Antibody

catalog :

APC-035-GP

clonality :

polyclonal

host :

guinea-pigs

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 3

Reference
Smith K, Murphy P, Jagger D. Divergent membrane properties of mouse cochlear glial cells around hearing onset. J Neurosci Res. 2021;99:679-698 pubmed publisher
Marangoni D, Yong Z, Kjellstrom S, Vijayasarathy C, A Sieving P, Bush R. Rearing Light Intensity Affects Inner Retinal Pathology in a Mouse Model of X-Linked Retinoschisis but Does Not Alter Gene Therapy Outcome. Invest Ophthalmol Vis Sci. 2017;58:1656-1664 pubmed publisher
Brasko C, Hawkins V, De La Rocha I, Butt A. Expression of Kir4.1 and Kir5.1 inwardly rectifying potassium channels in oligodendrocytes, the myelinating cells of the CNS. Brain Struct Funct. 2017;222:41-59 pubmed publisher

image

image 1 :

Western blot analysis of rat brain membranes (lanes 1 and 4), mouse brain membranes (lanes 2 and 5) and human SH-SY5Y neuroblastoma cell lysates (lanes 3 and 6): - 1-3. Guinea pig Anti-Kir4.1 (KCNJ10) Antibody (#APC-035-GP), (1:800).4-6. Guinea pig Anti-Kir4.1 (KCNJ10) Antibody, preincubated with Kir4.1/KCNJ10 Blocking Peptide (#BLP-PC035).

image 2 :

Expression of Kir4.1 in rat hippocampal dentate gyrus - Immunohistochemical staining of rat frozen brain sections using Guinea pig Anti-Kir4.1 (KCNJ10) Antibody (#APC-035-GP), (1:300). A. Kir4.1 staining (green) reveals Kir4.1 clusters in the molecular layer (Mol). B. The same section was stained with mouse anti-glial fibrillary acidic protein (GFAP), (red) showing astrocyte profiles. C. Merge of Kir4.1 and GFAP images reveals colocalization on some branches of astrocytic fibers. In addition, Kir4.1 is expressed in the fine ramification of astrocytic fibers, thus creating a “halo-like” cloud around the GFAP astrocytic outline. DAPI (blue) counterstain reveals the outline of the dentate granule layer.

product information

CAT :

APC-035-GP

SKU :

APC-035-GP-CF_0.2 ml

Product Name :

Guinea pig Anti-Kir4.1 (KCNJ10) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P49655

Applications :

IC IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Guinea Pig

Blocking Peptide :

BLP-PC035

Homology :

Human, mouse - identical

Formulation :

PBS pH7.4

isotype :

Guinea pig total IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)KLEE SLREQ AEKEG SALSV R, corresponding to amino acid residues 356-375 of rat Kir4.1

Product Page - Scientific background :

Kir4.1 is a member of the family of inward rectifying K+ channels. The family includes 15 members that are structurally and functionally different from the voltage-dependent K+ channels.The family's topology consists of two transmembrane domains that flank a single and highly conserved pore region with intracellular N- and C-termini. As is the case for the voltage-dependent K+ channels the functional unit for the Kir channels is composed of four subunits that can assemble as either homo or heteromers.Kir channels are characterized by a K+ efflux that is limited by depolarizing membrane potentials thus making them essential for controlling resting membrane potential and K+ homeostasis.Kir4.1 is a member of the Kir4 subfamily that includes one other member: Kir4.2. Kir4.1 can co-assemble with Kir4.2 but also with other Kir channels such as Kir2.1 and Kir5.1.The Kir4 subfamily has been classified as weak rectifiers with intermediate conductance. Kir4.1, encoded by KCNJ10, is mainly expressed in brain, specifically in glia cells, but also in retina, ear and kidney1,2.It has been proposed that Kir4.1 has an essential role in glial K+ buffering, a process that re-uptakes the K+ released during neuronal activity into the intracellular interstitial space. Loss of Kir4.1 causes retinal defects and loss of endochoclear potential3.

Applications may also work in :

IC IF IHC WB

Supplier :

Alomone Labs

Target :

ATP-sensitive inward rectifier potassium channel 10, KAB-2, BIR10, BIRK1, Kir1.2, Potassium channel inwardly rectifying subfamily J member 10

Short Description :

A Guinea Pig Polyclonal Antibody to Kir4.1 (KCNJ10) Channel

Long Description :

Guinea pig Anti-Kir4.1 (KCNJ10) Antibody (#APC-035-GP, formerly AGP-012) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize Kir4.1 potassium channel from human, rat, and mouse samples. The antigen used to immunize guinea pigs is the same as Anti-Kir4.1 (KCNJ10) Antibody (#APC-035) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation, etc.

Negative Control :

BLP-PC035

Positive Control :

Synonyms :

ATP-sensitive inward rectifier potassium channel 10, KAB-2, BIR10, BIRK1, Kir1.2, Potassium channel inwardly rectifying subfamily J member 10

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

KCNJ10

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

KO-Validated :

yes

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:300

Application Dilutions: Western blot wb :

1:800

more info or order :

Alomone Labs product webpage