Anti-Kir4.1 (KCNJ10)-ATTO Fluor-488 Antibody | Alomone Labs APC-035-AG product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Kir4.1 (KCNJ10)-ATTO Fluor-488 Antibody

catalog :

APC-035-AG

clonality :

polyclonal

host :

domestic rabbit

conjugate :

ATTO 488

clone name :

reactivity :

human, mouse, rat

application :

immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 8

Reference
Huang B, Zdora I, de Buhr N, Lehmbecker A, Baumg xe4 rtner W, Leitzen E. Phenotypical peculiarities and species-specific differences of canine and murine satellite glial cells of spinal ganglia. J Cell Mol Med. 2021;25:6909-6924 pubmed publisher
Wu P, Su X, Gao Z, Zhang D, Duan X, Xiao Y, et al. Renal Tubule Nedd4-2 Deficiency Stimulates Kir4.1/Kir5.1 and Thiazide-Sensitive NaCl Cotransporter in Distal Convoluted Tubule. J Am Soc Nephrol. 2020;31:1226-1242 pubmed publisher
Korrapati S, Taukulis I, Olszewski R, Pyle M, Gu S, Singh R, et al. Single Cell and Single Nucleus RNA-Seq Reveal Cellular Heterogeneity and Homeostatic Regulatory Networks in Adult Mouse Stria Vascularis. Front Mol Neurosci. 2019;12:316 pubmed publisher
Kinboshi M, Shimizu S, Mashimo T, Serikawa T, Ito H, Ikeda A, et al. Down-Regulation of Astrocytic Kir4.1 Channels during the Audiogenic Epileptogenesis in Leucine-Rich Glioma-Inactivated 1 (Lgi1) Mutant Rats. Int J Mol Sci. 2019;20: pubmed publisher
Kinboshi M, Mukai T, Nagao Y, Matsuba Y, Tsuji Y, Tanaka S, et al. Inhibition of Inwardly Rectifying Potassium (Kir) 4.1 Channels Facilitates Brain-Derived Neurotrophic Factor (BDNF) Expression in Astrocytes. Front Mol Neurosci. 2017;10:408 pubmed publisher
Sato M, Higuchi T, Nin F, Ogata G, Sawamura S, Yoshida T, et al. Hearing Loss Controlled by Optogenetic Stimulation of Nonexcitable Nonglial Cells in the Cochlea of the Inner Ear. Front Mol Neurosci. 2017;10:300 pubmed publisher
Silva J, Lopes A, Talbot J, Cecílio N, Rossato M, Silva R, et al. Neuroimmune-Glia Interactions in the Sensory Ganglia Account for the Development of Acute Herpetic Neuralgia. J Neurosci. 2017;37:6408-6422 pubmed publisher
Brasko C, Hawkins V, De La Rocha I, Butt A. Expression of Kir4.1 and Kir5.1 inwardly rectifying potassium channels in oligodendrocytes, the myelinating cells of the CNS. Brain Struct Funct. 2017;222:41-59 pubmed publisher

image

image 1 :

Expression of Kir4.1 in rat cerebellum - Immunohistochemical staining of frozen sections of rat cerebellum using Anti-Kir4.1 (KCNJ10)-ATTO Fluor-488 Antibody (#APC-035-AG) (green), (1:50). Staining is specific for Bergmann glial cells prolongations (white arrows) in the molecular layer (ML) and astrocytes (yellow arrows) in the granular layer (GL). Purkinje cell bodies are stained with fluorescent Nissl stain (red). Hoechst 33342 (blue) is used as counterstain.

image 2 :

Western blot analysis of rat heart membrane (lanes 1 and 3) rat spleen lysate (lanes 2 and 4) mouse heart membrane (lanes 5 and 6) and human Colo205 cell linelysate(lanes 7 and 8): - 1257. Guinea pig Anti-KCNQ1 Antibody (#AGP-050) (1:200).3468. Guinea pig Anti-KCNQ1 Antibody preincubated with the negative control antigen.

product information

CAT :

APC-035-AG

SKU :

APC-035-AG_50 mcl

Product Name :

Anti-Kir4.1 (KCNJ10)-ATTO Fluor-488 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P49655

Applications :

IF IHC

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PC035

Homology :

Human, mouse - identical

Formulation :

PBS pH7.4, 1% BSA with 0.05% sodium azide

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

50 µl double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

1% BSA, 0.05% NaN3

Immunogen Location :

Intracellular, C-terminus

Label :

ATTO-488. Maximum absorption 501 nm; maximum fluorescence 523 nm. The fluorescence is excited most efficiently in the 480 - 515 nm range. This label is analogous to the dye fluorescein isothiocyanate (FITC) and can be used with filters used to detect FITC.

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)KLEE SLREQ AEKEG SALSV R, corresponding to amino acid residues 356-375 of rat Kir4.1

Product Page - Scientific background :

Kir4.1 is a member of the inward rectifying K+ channel family. The family includes 15 members that are structurally and functionally different from the voltage-dependent K+ channels.The family's topology consists of two transmembrane domains that flank a single and highly conserved pore region with intracellular N- and C-termini. As is the case for the voltage-dependent K+ channels the functional unit for the Kir channels is composed of four subunit that can assembly as either homo or heteromers.Kir channels are characterized by a K+ efflux that is limited by depolarizing membrane potentials thus making them essential for controlling resting membrane potential and K+ homeostasis.Kir4.1 is a member of the Kir4 subfamily that includes one other member: Kir4.2. Kir4.1 can co-assemble with Kir4.2 but also with other Kir channels such as Kir2.1 and Kir5.1.The Kir4 subfamily has been classified as weak rectifiers with intermediate conductance. Kir4.1, encoded by KCNJ10, is mainly expressed in brain, specifically in glia cells, but also in retina, ear and kidney.1,2It has been proposed that Kir4.1 has an essential role in glial K+ buffering, a process that re-uptakes the K+ released during neuronal activity into the intracellular interstitial space. Loss of Kir4.1 causes retinal defects and loss of endochoclear potential.3

Applications may also work in :

IF IHC

Supplier :

Alomone Labs

Target :

ATP-sensitive inward rectifier potassium channel 10, KAB-2, BIR10, BIRK1, Kir1.2, Potassium channel inwardly rectifying subfamily J member 10

Short Description :

A Rabbit Polyclonal Antibody to Kir4.1 (KCNJ10) Channel Conjugated to the Fluorescent Dye ATTO-488

Long Description :

Anti-Kir4.1 (KCNJ10) Antibody (#APC-035) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunoprecipitation, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize Kir4.1 potassium channel from rat, mouse, and human samples. Anti-Kir4.1 (KCNJ10)-ATTO Fluor-488 Antibody (#APC-035-AG) is directly labeled with an ATTO-488 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The ATTO-488 label is analogous to the well known dye fluorescein isothiocyanate (FITC) and can be used with filters typically used to detect FITC. Anti-Kir4.1 (KCNJ10)-ATTO Fluor-488 Antibody is specially suited to experiments requiring simultaneous labeling of different markers.

Negative Control :

RIC-001-AG

Positive Control :

Synonyms :

ATP-sensitive inward rectifier potassium channel 10, KAB-2, BIR10, BIRK1, Kir1.2, Potassium channel inwardly rectifying subfamily J member 10

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

KCNJ10

Form :

Lyophilized powder

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

KO-Validated :

yes

Clone :

Standard quality control of each lot :

Western blot analysis (unlabeled antibody, #APC-035), and immunohistochemistry (labeled antibody).

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:50

Application Dilutions: Western blot wb :

Contact Alomone for information

more info or order :

Alomone Labs product webpage