Anti-sloβ2 (KCNMB2) Antibody | Alomone Labs APC-034 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-sloβ2 (KCNMB2) Antibody

catalog :

APC-034

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 4

Reference
Sanghvi S, Szteyn K, Ponnalagu D, Sridharan D, Lam A, Hansra I, et al. Inhibition of BKCa channels protects neonatal hearts against myocardial ischemia and reperfusion injury. Cell Death Discov. 2022;8:175 pubmed publisher
Yu M, Guo L, Li N, Henzel K, Gu H, Ran X, et al. Overexpression of Kcnmb2 in Dorsal CA1 of Offspring Mice Rescues Hippocampal Dysfunction Caused by a Methyl Donor-Rich Paternal Diet. Front Cell Neurosci. 2018;12:360 pubmed publisher
Kicinska A, Augustynek B, Kulawiak B, Jarmuszkiewicz W, Szewczyk A, Bednarczyk P. A large-conductance calcium-regulated K+ channel in human dermal fibroblast mitochondria. Biochem J. 2016;473:4457-4471 pubmed
Mizutani H, Yamamura H, Muramatsu M, Hagihara Y, Suzuki Y, Imaizumi Y. Modulation of Ca2+ oscillation and melatonin secretion by BKCa channel activity in rat pinealocytes. Am J Physiol Cell Physiol. 2016;310:C740-7 pubmed publisher

image

image 1 :

Western blot analysis of rat kidney (lanes 1 and 3) and heart (lanes 2 and 4) membranes: - 1,2. Anti-sloβ2 (KCNMB2) Antibody (#APC-034), (1:200).3,4. Anti-sloβ2 (KCNMB2) Antibody, preincubated with sloβ2/KCNMB2 Blocking Peptide (#BLP-PC034).

image 2 :

Expression of KCNMB2 in rat hippocampus - Immunohistochemical staining of rat hippocampus using Anti-sloβ2 (KCNMB2) Antibody (#APC-034). A. KCNMB2 appears in the pyramidal layer (green). B. Staining of interneurons with mouse anti-parvalbumin (PV, red). C. Confocal merge of KCNMB2 and PV demonstrates presence of KCNMB2 also in PV (GABA containing) cells (orange).

image 3 :

Expression of KCNMB2in rat hippocampus - Immunohistochemical staining of rat hippocampus usingAnti-slo?2 (KCNMB2)Antibody (#APC-034). A. KCNMB2appears in the pyramidal layer (green). B. Staining of interneurons with mouse anti-parvalbumin (PV red). C. Confocal merge of KCNMB2and PV demonstrates presence of KCNMB2also in PV (GABA containing) cells (orange).

product information

CAT :

APC-034

SKU :

APC-034-CF_0.2 ml

Product Name :

Anti-sloβ2 (KCNMB2) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q9Y691

Applications :

IC IF IHC WB

Reactivity :

Human Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PC034

Homology :

Rat, dog - identical; mouse - 18/19 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, N-terminal domain

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

(C)RHDEKRNIYQKIRDHDLLD, corresponding to amino acid residues 14-32 of human KCNMB2

Product Page - Scientific background :

sloβ2 is a member the regulatory β subunit family that controls the activity of the large conductance Ca2+-activated K+ channel KCa1.1. This family includes four members with a shared topology: two trans-membrane domains, short intracellular N- and C-termini and a large extracellular region and a distinct tissue distribution.sloβ2 expression is relatively broad and includes expression in the brain, heart, kidney, adrenal chromaffin cells and ovary.The KCa1.1 K+ channel can be activated by either an increase in intracellular Ca2+ concentration or by membrane depolarization. The regulatory β subunits increase the sensitivity of the pore-forming KCa1.1 subunit to Ca2+ and membrane voltage and they may also change the channel pharmacology.The sloβ2 subunit is unique in that it is able to induce a rapid and complete inactivation of the KCa1.1 channel in a manner that closely resembles the ball-and-chain inactivation of the voltage-dependent K+ (KV) channels. In other words, the inactivation is dependent on a sequence in the N-terminal part of the sloβ2 subunit that appears to block the mouth of the ion permeation pathway.The physiological significance of the sloβ2 subunit is not clear, but it appears to participate in the inactivation of the KCa1.1 channel in hippocampal CA1 neurons and adrenal chromaffin cells.

Applications may also work in :

IC IF IHC WB

Supplier :

Alomone Labs

Target :

Ca2+-activated K+ channel subunit β2, BKCa β2, BK beta 2, Maxi K channel β2, Charybdotoxin receptor subunit beta-2

Short Description :

A Rabbit Polyclonal Antibody to slo?2 (KCNMB2) Subunit

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of human sloβ2. Anti-sloβ2 (KCNMB2) Antibody (#APC-034) can be used in western blot, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize sloβ2 from human, rat, and mouse samples.

Negative Control :

BLP-PC034

Positive Control :

Synonyms :

Ca2+-activated K+ channel subunit β2, BKCa β2, BK beta 2, Maxi K channel β2, Charybdotoxin receptor subunit beta-2

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

KCNMB2

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage