Anti-Kir2.3 (KCNJ4) Antibody | Alomone Labs APC-032 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Kir2.3 (KCNJ4) Antibody

catalog :

APC-032

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 4

Reference
Pluteanu F, Seidl M, Hamer S, Scholz B, Müller F. Inward Rectifier K+ Currents Contribute to the Proarrhythmic Electrical Phenotype of Atria Overexpressing Cyclic Adenosine Monophosphate Response Element Modulator Isoform CREM-IbΔC-X. J Am Heart Assoc. 2020;9:e016144 pubmed publisher
Sengupta S, Rothenberg K, Li H, Hoffman B, Bursac N. Altering integrin engagement regulates membrane localization of Kir2.1 channels. J Cell Sci. 2019;132: pubmed publisher
Xia P, Li L, Qi R, Shi J, Ju W, Chen M. Electrical and histological remodeling of the pulmonary vein in 2K1C hypertensive rats: Indication of initiation and maintenance of atrial fibrillation. Anatol J Cardiol. 2018;19:169-175 pubmed publisher
Murata Y, Yasaka T, Takano M, Ishihara K. Neuronal and glial expression of inward rectifier potassium channel subunits Kir2.x in rat dorsal root ganglion and spinal cord. Neurosci Lett. 2016;617:59-65 pubmed publisher

image

image 1 :

Western blot analysis of rat kidney (lanes 1 and 3) and brain (lanes 2 and 4) membranes: - 1,2. Anti-Kir2.3 (KCNJ4) Antibody (#APC-032), (1:200)3,4. Anti-Kir2.3 (KCNJ4) Antibody, preincubated with Kir2.3/KCNJ4 Blocking Peptide (#BLP-PC032).

image 2 :

Peptide (C)EFGS HLDLE RMQAA TLPLD N corresponding to amino acid residues 418-437 of rat Kir2.3 (AccessionP52190). Intracellular C-terminal part.

image 3 :

Expression of Kir2.1 and Kir2.3 channels in Lamina I pacemaker cells. - Immunohistochemical staining of rat spinal cord sections using Lamina I pacemaker using Anti-Kir2.1 (KCNJ2) Antibody (#APC-026) and Anti-Kir2.3 (KCNJ4) Antibody (#APC-032). Confocal images of representative biocytin-filled pacemaker neurons (red) processed for Kir (green). Merged images demonstrate that immunoreactive puncta for Kir2.1 and Kir2.3 (yellow; inset) are localized to identified pacemaker neurons within lamina I of the neonatal spinal cord (right). Scale bars: 10 ?m; inset 2 ?m.Adapted from Li J. et al. (2013) with permission of The Society for Neuroscience.

product information

CAT :

APC-032

SKU :

APC-032-CF_0.2 ml

Product Name :

Anti-Kir2.3 (KCNJ4) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P52190

Applications :

IC IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PC032

Homology :

Mouse - identical; guinea pig - 19/20 amino acid residues identical; hamster, human, Xenopus - 18/20 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminal domain

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)EFGS HLDLE RMQAA TLPLD N, corresponding to amino acid residues 418-437 of rat Kir2.3

Product Page - Scientific background :

Kir2.3 is a member of the family of inward rectifying K+ channels. The family includes 15 members that are structurally and functionally different from the voltage-dependent K+ channels.The family's topology consists of two transmembrane domains that flank a single and highly conserved pore region with intracellular N- and C-termini. As is the case for the voltage-dependent K+ channels the functional unit for the Kir channels is composed of four subunit that can assembly as either homo or heterotetramers.Kir channels are characterized by a K+ efflux that is limited by depolarizing membrane potentials thus making them essential for controlling resting membrane potential and K+ homeostasis.Kir2.3 is a member of the Kir2.x subfamily that includes four members (Kir2.1- Kir2.4) that are characterized by strong inward rectification and high constitutive activity.Kir2.3 is expressed in a variety of tissues including heart and brain. 2In the heart, Kir2.3 forms heteromers with Kir2.1 and underlay the IK1 current (at least in some species) that is responsible for setting the resting membrane potential, preventing membrane hyperpolarization due to Na+ pump activity, influencing propagation velocity, altering the electrical space constant, and promoting late phase repolarization.1,2

Applications may also work in :

IC IF IHC WB

Supplier :

Alomone Labs

Target :

Inward rectifier potassium channel 4, BIR11, IRK3, HIRK2, HRK1

Short Description :

A Rabbit Polyclonal Antibody to Kir2.3 (KCNJ4) Channel

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of the rat Kir2.3 channel. Anti-Kir2.3 (KCNJ4) Antibody (#APC-032) can be used in western blot, immunocytochemistry, and immunohistochemistry applications. It has been designed to recognize Kir2.3 from human, rat, and mouse samples.

Negative Control :

BLP-PC032

Positive Control :

Synonyms :

Inward rectifier potassium channel 4, BIR11, IRK3, HIRK2, HRK1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

KCNJ4

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IP IHC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage