Anti-HCN2 Antibody | Alomone Labs APC-030 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-HCN2 Antibody

catalog :

APC-030

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

Alomone Labs product webpage

citations: 24

Published Application/Species/Sample/Dilution	Reference
western blot; rat; 1:500; loading ...; fig 4b	Liu D, Song A, Qi X, van Vliet P, Xiao J, Xiong F, et al. Cohesin-protein Shugoshin-1 controls cardiac automaticity via HCN4 pacemaker channel. Nat Commun. 2021;12:2551 pubmed publisher
immunohistochemistry; rat; 1:100; loading ...; fig 3f	Zhao Z, Zhang K, Liu X, Yan H, Ma X, Zhang S, et al. Involvement of HCN Channel in Muscarinic Inhibitory Action on Tonic Firing of Dorsolateral Striatal Cholinergic Interneurons. Front Cell Neurosci. 2016;10:71 pubmed publisher
western blot; mouse; 1:1000; fig 4	Schwenk J, PÃ©rez Garci E, Schneider A, Kollewe A, Gauthier Kemper A, Fritzius T, et al. Modular composition and dynamics of native GABAB receptors identified by high-resolution proteomics. Nat Neurosci. 2016;19:233-42 pubmed publisher
	Lezmy J, Arancibia Carcamo I, Quintela López T, Sherman D, Brophy P, Attwell D. Astrocyte Ca2+-evoked ATP release regulates myelinated axon excitability and conduction speed. Science. 2021;374:eabh2858 pubmed publisher
	Tanwar M, Kateriya S, Nair D, Jose M. Optogenetic modulation of real-time nanoscale dynamics of HCN channels using photoactivated adenylyl cyclases. RSC Chem Biol. 2021;2:863-875 pubmed publisher
	Chen C, Liu L, Shu Y, Jing P, Lu Y, Zhang X, et al. Blockade of HCN2 Channels Provides Neuroprotection Against Ischemic Injury via Accelerating Autophagic Degradation in Hippocampal Neurons. Neurosci Bull. 2020;36:875-894 pubmed publisher
	Schoepf C, Zeidler M, Spiecker L, Kern G, Lechner J, Kummer K, et al. Selected Ionotropic Receptors and Voltage-Gated Ion Channels: More Functional Competence for Human Induced Pluripotent Stem Cell (iPSC)-Derived Nociceptors. Brain Sci. 2020;10: pubmed publisher
	Madden J, Davis O, Boyle K, Iredale J, Browne T, Callister R, et al. Functional and Molecular Analysis of Proprioceptive Sensory Neuron Excitability in Mice. Front Mol Neurosci. 2020;13:36 pubmed publisher
	Brandalise F, Kalmbach B, Mehta P, Thornton O, Johnston D, Zemelman B, et al. Fragile X Mental Retardation Protein Bidirectionally Controls Dendritic Ih in a Cell Type-Specific Manner between Mouse Hippocampus and Prefrontal Cortex. J Neurosci. 2020;40:5327-5340 pubmed publisher
	Kharouf Q, Phillips A, Bleakley L, Morrisroe E, Oyrer J, Jia L, et al. The hyperpolarization-activated cyclic nucleotide-gated 4 channel as a potential anti-seizure drug target. Br J Pharmacol. 2020;177:3712-3729 pubmed publisher
	Leon Aparicio D, Salvador C, Aparicio Trejo O, Briones Herrera A, Pedraza Chaverri J, Vaca L, et al. Novel Potassium Channels in Kidney Mitochondria: The Hyperpolarization-Activated and Cyclic Nucleotide-Gated HCN Channels. Int J Mol Sci. 2019;20: pubmed publisher
	Hammelmann V, Stieglitz M, Hülle H, Le Meur K, Kass J, Brümmer M, et al. Abolishing cAMP sensitivity in HCN2 pacemaker channels induces generalized seizures. JCI Insight. 2019;4: pubmed publisher
	Dini L, Del Lungo M, Resta F, Melchiorre M, Spinelli V, Di Cesare Mannelli L, et al. Selective Blockade of HCN1/HCN2 Channels as a Potential Pharmacological Strategy Against Pain. Front Pharmacol. 2018;9:1252 pubmed publisher
	Hofmann L, Hose D, Grießhammer A, Blum R, Döring F, Dib Hajj S, et al. Characterization of small fiber pathology in a mouse model of Fabry disease. elife. 2018;7: pubmed publisher
	Zhuang Q, Li G, Li B, Zhang C, Zhang X, Xi K, et al. Regularizing firing patterns of rat subthalamic neurons ameliorates parkinsonian motor deficits. J Clin Invest. 2018;128:5413-5427 pubmed publisher
	David F, Carçak N, Furdan S, Onat F, Gould T, Mészáros A, et al. Suppression of Hyperpolarization-Activated Cyclic Nucleotide-Gated Channel Function in Thalamocortical Neurons Prevents Genetically Determined and Pharmacologically Induced Absence Seizures. J Neurosci. 2018;38:6615-6627 pubmed publisher
	Silbernagel N, Walecki M, Schafer M, Kessler M, Zobeiri M, Rinné S, et al. The VAMP-associated protein VAPB is required for cardiac and neuronal pacemaker channel function. FASEB J. 2018;32:6159-6173 pubmed publisher
	Silveira Villarroel H, Bompolaki M, Mackay J, Miranda Tapia A, Michaelson S, Leitermann R, et al. NPY Induces Stress Resilience via Downregulation of Ih in Principal Neurons of Rat Basolateral Amygdala. J Neurosci. 2018;38:4505-4520 pubmed publisher
	FISHER D, Luu P, Agarwal N, Kurz J, Chetkovich D. Loss of HCN2 leads to delayed gastrointestinal motility and reduced energy intake in mice. PLoS ONE. 2018;13:e0193012 pubmed publisher
	Stieglitz M, Fenske S, Hammelmann V, Becirovic E, Schöttle V, Delorme J, et al. Disturbed Processing of Contextual Information in HCN3 Channel Deficient Mice. Front Mol Neurosci. 2017;10:436 pubmed publisher
	Zobeiri M, Chaudhary R, Datunashvili M, Heuermann R, Lüttjohann A, Narayanan V, et al. Modulation of thalamocortical oscillations by TRIP8b, an auxiliary subunit for HCN channels. Brain Struct Funct. 2018;223:1537-1564 pubmed publisher
	Zhang M, Vollmer C, Nurse C. Adenosine and dopamine oppositely modulate a hyperpolarization-activated current Ih in chemosensory neurons of the rat carotid body in co-culture. J Physiol. 2018;596:3101-3117 pubmed publisher
	Negrini D, Marcozzi C, Solari E, Bossi E, Cinquetti R, Reguzzoni M, et al. Hyperpolarization-activated cyclic nucleotide-gated channels in peripheral diaphragmatic lymphatics. Am J Physiol Heart Circ Physiol. 2016;311:H892-H903 pubmed publisher
	Leist M, Datunashvilli M, Kanyshkova T, Zobeiri M, Aissaoui A, Cerina M, et al. Two types of interneurons in the mouse lateral geniculate nucleus are characterized by different h-current density. Sci Rep. 2016;6:24904 pubmed publisher

image

image 1 :

Western blot analysis of rat brain membranes: - 1. Anti-HCN2 Antibody (#APC-030), (1:200).2. Anti-HCN2 Antibody, preincubated with HCN2 Blocking Peptide (#BLP-PC030).

image 2 :

Expression of HCN2 in rat cerebellum - Immunochistochemical staining of rat cerebellum frozen sections using Anti-HCN2 Antibody (#APC-030 ). A. HCN2 (red) appears in Purkinje cells (arrows). B. Staining of astrocytes with mouse anti-glial fibrillary acidic protein (GFAP, green demonstrates the restriction of HCN2 to neuronal cell bodies. C. Confocal merge of HCN2 and GFAP images demonstrates the respective localization of these proteins.

image 3 :

Expression of HCN2 in rat DRG primary culture - Immunocytochemical staining of paraformaldehyde-fixed and permeabilized rat dorsal root ganglion (DRG) primary culture usingAnti-HCN2Antibody (#APC-030) (1:100) (green). Cells were stained with Anti-HCN2 Antibodyfollowed by goat anti-rabbit-AlexaFluor-488 secondary antibody. Nuclear staining of cells using the cell-permeable DNA dye Hoechst 33342 (blue).

product information

CAT :

APC-030

SKU :

APC-030-CF_0.2 ml

Product Name :

Anti-HCN2 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q9UL51

Applications :

IC IF IHC IP WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PC030

Homology :

Rat, mouse - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

(C)EEAGPAGEPRGSQAS, corresponding to amino acid residues 147-161 of human HCN2

Product Page - Scientific background :

Hyperpolarization-activated cation currents (Ih) appear in the heart and the brain having crucial role in controlling electrical pacemaker activity, contributing to biological processes such as heartbeat, sleep-wake cycle and synaptic plasticity.1,2The Ih currents are generated by the hyperpolarization-activated cyclic nucleotide-gated channel family (HCN), which comprises four homologous members, named HCN1-4. Each HCN subunit consist of six transmembrane domains (TM), a pore region between TM5-TM6 and a binding domain to cyclic nucleotides (CNBD) in the cytoplasmic C-terminus.2The HCN subunits can form functional homomers and can also co-assemble into functional heteromers.2The channels are closely related to each other and share homology of about 60%. However, they are diverging from each other in their cytoplasmic N- and the C-terminus. The channels HCN1-4 mainly differ from each other with regard to their speed of activation and the extent by which they are modulated by cAMP. HCN1 is the fastest channel, followed by HCN2, HCN3 and HCN4.2,3HCN2 is the most abundant neuronal channel and is found almost ubiquitously in the brain.3

Applications may also work in :

IC IF IHC IP WB

Supplier :

Alomone Labs

Target :

Hyperpolarization-activated cyclic nucleotide-gated potassium channel 2, BCNG2, HAC-1

Short Description :

A Rabbit Polyclonal Antibody to HCN2 Channel

Long Description :

Anti-HCN2 Antibody (#APC-030) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot, immunoprecipitation, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize HCN2 from human, rat, and mouse samples.

Negative Control :

BLP-PC030

Positive Control :

Synonyms :

Hyperpolarization-activated cyclic nucleotide-gated potassium channel 2, BCNG2, HAC-1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

HCN2

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

KO-Validated :

yes

Cited Application :

IP IHC ICC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage