Anti-KCNN2 (KCa2.2, SK2) Antibody | Alomone Labs APC-028 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-KCNN2 (KCa2.2, SK2) Antibody

catalog :

APC-028

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

Alomone Labs product webpage

citations: 15

Published Application/Species/Sample/Dilution	Reference
western blot; mouse; 1:500; loading ...; fig 6a	Deng P, Carlin D, Oh Y, Myrick L, Warren S, Cavalli V, et al. Voltage-Independent SK-Channel Dysfunction Causes Neuronal Hyperexcitability in the Hippocampus of Fmr1 Knock-Out Mice. J Neurosci. 2019;39:28-43 pubmed publisher
	Bach E, Morgan J, Ewin S, Barth S, Raab Graham K, Weiner J. Chronic Ethanol Exposures Leads to a Negative Affective State in Female Rats That Is Accompanied by a Paradoxical Decrease in Ventral Hippocampus Excitability. Front Neurosci. 2021;15:669075 pubmed publisher
	Liu Y, Xing H, Wilkes B, Yokoi F, Chen H, Vaillancourt D, et al. The abnormal firing of Purkinje cells in the knockin mouse model of DYT1 dystonia. Brain Res Bull. 2020;165:14-22 pubmed publisher
	Park J, Jang K, Park K. Apamin Suppresses LPS-Induced Neuroinflammatory Responses by Regulating SK Channels and TLR4-Mediated Signaling Pathways. Int J Mol Sci. 2020;21: pubmed publisher
	Sun J, Liu Y, Zhu G, Cato C, Hao X, Qian L, et al. PKA and Ube3a regulate SK2 channel trafficking to promote synaptic plasticity in hippocampus: Implications for Angelman Syndrome. Sci Rep. 2020;10:9824 pubmed publisher
	Niibori M, Kudo Y, Hayakawa T, Ikoma Seki K, Kawamata R, Sato A, et al. Mechanism of aspirin-induced inhibition on the secondary hyperalgesia in osteoarthritis model rats. Heliyon. 2020;6:e03963 pubmed publisher
	Mi Z, Yang J, He Q, Zhang X, Xiao Y, Shu Y. Alterations of Electrophysiological Properties and Ion Channel Expression in Prefrontal Cortex of a Mouse Model of Schizophrenia. Front Cell Neurosci. 2019;13:554 pubmed publisher
	Ling T, Yi F, Lu T, Wang X, Sun X, Willis M, et al. F-box protein-32 down-regulates small-conductance calcium-activated potassium channel 2 in diabetic mouse atria. J Biol Chem. 2019;294:4160-4168 pubmed publisher
	Ewin S, Morgan J, Niere F, McMullen N, Barth S, Almonte A, et al. Chronic Intermittent Ethanol Exposure Selectively Increases Synaptic Excitability in the Ventral Domain of the Rat Hippocampus. Neuroscience. 2019;398:144-157 pubmed publisher
	Chen M, Yin D, Guo S, Xu D, Wang Z, Chen Z, et al. Sex-specific activation of SK current by isoproterenol facilitates action potential triangulation and arrhythmogenesis in rabbit ventricles. J Physiol. 2018;596:4299-4322 pubmed publisher
	Fan X, Yu Y, Lan H, Ou X, Yang L, Li T, et al. Ca2+/Calmodulin-Dependent Protein Kinase II (CaMKII) Increases Small-Conductance Ca2+-Activated K+ Current in Patients with Chronic Atrial Fibrillation. Med Sci Monit. 2018;24:3011-3023 pubmed publisher
	Ko J, Guo S, Hassel J, Celestino Soper P, Lynnes T, Tisdale J, et al. Ondansetron blocks wild-type and p.F503L variant small-conductance Ca2+-activated K+ channels. Am J Physiol Heart Circ Physiol. 2018;315:H375-H388 pubmed publisher
	Lu T, Wang X, Chai Q, Sun X, Sieck G, Katusic Z, et al. Role of the endothelial caveolae microdomain in shear stress-mediated coronary vasorelaxation. J Biol Chem. 2017;292:19013-19023 pubmed publisher
	Torrente A, Zhang R, Wang H, Zaini A, Kim B, Yue X, et al. Contribution of small conductance K+ channels to sinoatrial node pacemaker activity: insights from atrial-specific Na+ /Ca2+ exchange knockout mice. J Physiol. 2017;595:3847-3865 pubmed publisher
	Willis M, Trieb M, Leitner I, Wietzorrek G, Marksteiner J, Knaus H. Small-conductance calcium-activated potassium type 2 channels (SK2, KCa2.2) in human brain. Brain Struct Funct. 2017;222:973-979 pubmed publisher

image

image 1 :

Western blot analysis of rat brain membranes: - 1. Anti-KCNN2 (KCa2.2, SK2) Antibody #APC-028), (1:200).2. Anti-KCNN2 (KCa2.2, SK2) Antibody, preincubated with KCNN2/KCa2.2 Blocking Peptide (#BLP-PC028).

image 2 :

Expression of KCNN2 (SK2) in rat cerebral cortex - Immunohistochemical staining of KCNN2 channels using Anti-KCNN2 (KCa2.2, SK2) Antibody (#APC-028) in rat cerebral cortex. Picture showing the the third layer. Note that both neural (black arrows) and glial cells (green arrows) show intense staining. Reaction product is red and counterstain is H&E.

image 3 :

Western blot analysisof rat brain membranes: - 1.Anti-KCNN2 (KCa2.2 SK2)Antibody #APC-028) (1:200).2. Anti-KCNN2 (KCa2.2 SK2) Antibody preincubated with the negative control antigen.

product information

CAT :

APC-028

SKU :

APC-028-CF_0.2 ml

Product Name :

Anti-KCNN2 (KCa2.2, SK2) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P70604

Applications :

IC IF IHC IP WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PC028

Homology :

Mouse - 17/18 amino acid residues identical; human - 16/18 amino acid residues identical; chicken - 14/18 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminal domain

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)ETQMENYDKHVTYNAERS, corresponding to amino acid residues 542-559 of rat KCNN2

Product Page - Scientific background :

KCa2.2 (KCNN2, SK2) is a member of the Ca2+-activated K+ channel family with small conductance that includes KCa2.1 (KCNN1, SK1) and KCa2.3 (KCNN3, SK3). The channel is voltage insensitive and is activated by intracellular Ca2+ in the submicromolar range. It has, though, a similar topology to that of voltage-dependent K+ channels (KV channels), that is six transmembrane domains and intracellular N- and C-termini. The functional channel of all the KCa2 family members is a multimeric protein composed of four pore-forming subunits.KCa2 channels are extremely sensitive to the levels of intracellular Ca2+ and concentrations as low as 300-700 nM can open the channels very rapidly (5-15 ms). Hence, the KCa2 channels are highly sensitive and fast Ca2+ sensors resembling other known Ca2+-binding proteins. This type of Ca2+-dependent activation is achieved by the constitutive binding of the KCa2 channels to calmodulin, a highly expressed Ca2+-binding protein via a calmodulin-binding domain situated at the cytoplasmic C-terminus.Pharmacologically, the KCa2 channels are the only known targets of the bee venom toxin Apamin, with KCa2.1 being the least sensitive, KCa2.2 the most sensitive and KCa2.3 showing intermediate sensitivity.KCa2.2 is predominantly expressed in the nervous system although expression in endothelial cells, heart and liver has been described.KCa2.2 is known to be involved in the regulation of neuronal excitability. They do so mainly via a phenomenon known as after hyperpolarization in which KCa2 channels open in response to increased intracellular Ca2+ concentrations that result from the entry of extracellular Ca2+ through voltage-dependent Ca2+ channels during action potentials. In this way, KCa2 channels effectively form a Ca2+-mediated feedback loop.

Applications may also work in :

IC IF IHC IP WB

Supplier :

Alomone Labs

Target :

SKCa2, Small conductance calcium-activated potassium channel protein 2, Apamin-sensitive small conductance Ca2+-dependent K+ channel

Short Description :

A Rabbit Polyclonal Antibody to KCNN2 (KCa2.2, SK2) Channel

Long Description :

Anti-KCNN2 (KCa2.2, SK2) Antibody (#APC-028) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunocytochemistry, immunohistochemistry, and immunoprecipitation applications. It has been designed to recognize KCNN2 from human, rat, and mouse samples.

Negative Control :

BLP-PC028

Positive Control :

Synonyms :

SKCa2, Small conductance calcium-activated potassium channel protein 2, Apamin-sensitive small conductance Ca2+-dependent K+ channel

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

KCNN2

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

ICC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage