Guinea pig Anti-KCNN2 (KCa2.2, SK2) Antibody | Alomone Labs APC-028-GP product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Guinea pig Anti-KCNN2 (KCa2.2, SK2) Antibody

catalog :

APC-028-GP

clonality :

polyclonal

host :

guinea-pigs

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot

more info or order :

Alomone Labs product webpage

citations: 2

Reference
Darche F, Rivinius R, Rahm A, Köllensperger E, Leimer U, Germann G, et al. In vivo cardiac pacemaker function of differentiated human mesenchymal stem cells from adipose tissue transplanted into porcine hearts. World J Stem Cells. 2020;12:1133-1151 pubmed publisher
Sun J, Liu Y, Zhu G, Cato C, Hao X, Qian L, et al. PKA and Ube3a regulate SK2 channel trafficking to promote synaptic plasticity in hippocampus: Implications for Angelman Syndrome. Sci Rep. 2020;10:9824 pubmed publisher

image

image 1 :

Western blot analysis of rat brain lysate (lanes 1 and 3) and mouse brain lysate (lanes 2 and 4): - 1,2. Guinea pig Anti-KCNN2 (KCa2.2, SK2) Antibody (#APC-028-GP), (1:1000).3,4. Guinea pig Anti-KCNN2 (KCa2.2, SK2) Antibody, preincubated with KCNN2/KCa2.2 Blocking Peptide (#BLP-PC028).

product information

CAT :

APC-028-GP

SKU :

APC-028-GP-CF_0.2 ml

Product Name :

Guinea pig Anti-KCNN2 (KCa2.2, SK2) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P70604

Applications :

Reactivity :

Rat Mouse

Host :

Guinea Pig

Blocking Peptide :

BLP-PC028

Homology :

Mouse - 17/18 amino acid residues identical; human - 16/18 amino acid residues identical; chicken - 14/18 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Guinea pig total IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminal domain

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)ETQMENYDKHVTYNAERS, corresponding to amino acid residues 542-559 of rat KCNN2

Product Page - Scientific background :

KCa2.2 (KCNN2, SK2) is a member of the Ca2+-activated K+ channel family with small conductance that includes KCa2.1 (KCNN1, SK1) and KCa2.3 (KCNN3, SK3). The channel is voltage insensitive and is activated by intracellular Ca2+ in the submicromolar range. It has, though, a similar topology to that of voltage-dependent K+ channels (KV channels), that is six transmembrane domains and intracellular N- and C-termini. The functional channel of all the KCa2 family members is a multimeric protein composed of four pore-forming subunits.KCa2 channels are extremely sensitive to the levels of intracellular Ca2+ and concentrations as low as 300-700 nM can open the channels very rapidly (5-15 ms). Hence, the KCa2 channels are highly sensitive and fast Ca2+ sensors resembling other known Ca2+-binding proteins. This type of Ca2+-dependent activation is achieved by the constitutive binding of the KCa2 channels to calmodulin, a highly expressed Ca2+-binding protein via a calmodulin-binding domain situated at the cytoplasmic C-terminus.Pharmacologically, the KCa2 channels are the only known targets of the bee venom toxin Apamin, with KCa2.1 being the least sensitive, KCa2.2 the most sensitive and KCa2.3 showing intermediate sensitivity.KCa2.2 is predominantly expressed in the nervous system although expression in endothelial cells, heart and liver has been described.KCa2.2 is known to be involved in the regulation of neuronal excitability. They do so mainly via a phenomenon known as after hyperpolarization in which KCa2 channels open in response to increased intracellular Ca2+ concentrations that result from the entry of extracellular Ca2+ through voltage-dependent Ca2+ channels during action potentials. In this way, KCa2 channels effectively form a Ca2+-mediated feedback loop.

Applications may also work in :

Supplier :

Alomone Labs

Target :

SKCa2, Small conductance calcium-activated potassium channel protein 2, Apamin-sensitive small conductance Ca2+-dependent K+ channel

Short Description :

A Guinea Pig Polyclonal Antibody to KCNN2 (KCa2.2, SK2) Channel

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat KCNN2 channel. Guinea pig Anti-KCNN2 (KCa2.2, SK2) Antibody (#APC-028-GP, formerly AGP-045) can be used in western blot analysis. It has been designed to recognize KCNN2 from human, rat and mouse samples. The antigen used to immunize guinea pigs is the same as Anti-KCNN2 (KCa2.2, SK2) Antibody (#APC-028) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation, etc.

Negative Control :

BLP-PC028

Positive Control :

Synonyms :

SKCa2, Small conductance calcium-activated potassium channel protein 2, Apamin-sensitive small conductance Ca2+-dependent K+ channel

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

KCNN2

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:1000

more info or order :

Alomone Labs product webpage