Anti-KCNJ5 (Kir3.4) Antibody | Alomone Labs APC-027 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-KCNJ5 (Kir3.4) Antibody

catalog :

APC-027

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 3

Reference
Pluteanu F, Seidl M, Hamer S, Scholz B, Müller F. Inward Rectifier K+ Currents Contribute to the Proarrhythmic Electrical Phenotype of Atria Overexpressing Cyclic Adenosine Monophosphate Response Element Modulator Isoform CREM-IbΔC-X. J Am Heart Assoc. 2020;9:e016144 pubmed publisher
Xie D, Geng L, Xiong K, Zhao T, Wang S, Xue J, et al. Cold-Inducible RNA-Binding Protein Prevents an Excessive Heart Rate Response to Stress by Targeting Phosphodiesterase. Circ Res. 2020;126:1706-1720 pubmed publisher
Hardege I, Long L, Al Maskari R, Figg N, O Shaughnessy K. Targeted disruption of the Kcnj5 gene in the female mouse lowers aldosterone levels. Clin Sci (Lond). 2018;132:145-156 pubmed publisher

image

image 1 :

Western blot analysis of rat heart membranes: - 1. Anti-KCNJ5 (Kir3.4) Antibody (#APC-027), (1:200).2. Anti-KCNJ5 (Kir3.4) Antibody, preincubated with KCNJ5/Kir3.4 Blocking Peptide (#BLP-PC027).

image 2 :

Western blot analysisof rat heart membranes: - 1.Anti-KCNJ5 (Kir3.4)Antibody (#APC-027)(1:200).2. Anti-KCNJ5 (Kir3.4) Antibody preincubated with the negative control antigen.

product information

CAT :

APC-027

SKU :

APC-027-CF_0.2 ml

Product Name :

Anti-KCNJ5 (Kir3.4) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P48548

Applications :

IC IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PC027

Homology :

Mouse, human, pig - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

RNAMNQDMEIGVT(C), corresponding to amino acid residues 6-18 of rat Kir3.4

Product Page - Scientific background :

Kir3.4 is a member of the G-protein regulated inward-rectifier K+ (GIRK) channel subfamily which is part of an inward-rectifier K+ channel superfamily. The GIRK subfamily comprises four members in mammals (Kir3.1- Kir3.4) that present the common topology of the inward-rectifier superfamily: two transmembrane domains flanking a highly conserved pore region with the N and C-terminus located intracellularly.Kir3.4 and the other Kir3 family subunits can be activated by neurotransmitters and other factors via the activation of G-protein coupled receptors. Binding of the corresponding ligand to the G-protein receptor induces the dissociation of G α-GTP from the Gβγ dimer. The latter directly binds to Kir3 and activates the channel. Kir3.4 expression is largely confined to the heart, where it co-assembles with Kir3.1 to form the prototypical muscarinic-gated K+ channel KACh. Indeed, knockout mice for Kir3.4 showed impaired heart rate following vagal nerve stimulation.A peptide toxin originating from the Apis mellifera bee venom, Tertiapin (#STT-250) was shown to be a potent blocker of Kir3.4 containing channels (8.6 nM for the Kir3.1/3.4 combination).4

Applications may also work in :

IC IF IHC WB

Supplier :

Alomone Labs

Target :

GIRK4, G protein-activated inward rectifier potassium channel 4, Cardiac inward rectifier, CIR, KATP-1

Short Description :

A Rabbit Polyclonal Antibody to KCNJ5 (Kir3.4, GIRK4) Channel

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of the rat Kir3.4 channel. Anti-KCNJ5 (Kir3.4) Antibody (#APC-027) can be used in western blot and immunohistochemistry applications. It has been designed to recognize Kir3.4 from human, rat and mouse samples.

Negative Control :

BLP-PC027

Positive Control :

Synonyms :

GIRK4, G protein-activated inward rectifier potassium channel 4, Cardiac inward rectifier, CIR, KATP-1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

KCNJ5

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

KO-Validated :

yes

Cited Application :

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage