Anti-Kir2.1/KCNJ2 Antibody | Alomone Labs APC-026 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Kir2.1/KCNJ2 Antibody

catalog :

APC-026

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

Alomone Labs product webpage

citations: 14

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; mouse; 1:400; loading ...; fig 4a	Vila A, Shihabeddin E, Zhang Z, Santhanam A, Ribelayga C, O BRIEN J. Synaptic Scaffolds, Ion Channels and Polyamines in Mouse Photoreceptor Synapses: Anatomy of a Signaling Complex. Front Cell Neurosci. 2021;15:667046 pubmed publisher
western blot; mouse; 1:1000; loading ...; fig 5s1a	Lieberman O, Frier M, McGuirt A, Griffey C, Rafikian E, Yang M, et al. Cell-type-specific regulation of neuronal intrinsic excitability by macroautophagy. elife. 2020;9: pubmed publisher
	Simon Chica A, Fern xe1 ndez M, W xfc lfers E, Lother A, Hilgendorf I, Seemann G, et al. Novel insights into the electrophysiology of murine cardiac macrophages: relevance of voltage-gated potassium channels. Cardiovasc Res. 2022;118:798-813 pubmed publisher
	Kabakov A, Sengun E, Lu Y, Roder K, Bronk P, Baggett B, et al. Three-Week-Old Rabbit Ventricular Cardiomyocytes as a Novel System to Study Cardiac Excitation and EC Coupling. Front Physiol. 2021;12:672360 pubmed publisher
	Liu M, Liu H, Parthiban P, Kang G, Shi G, Feng F, et al. Inhibition of the unfolded protein response reduces arrhythmia risk after myocardial infarction. J Clin Invest. 2021;131: pubmed publisher
	Sengupta S, Rothenberg K, Li H, Hoffman B, Bursac N. Altering integrin engagement regulates membrane localization of Kir2.1 channels. J Cell Sci. 2019;132: pubmed publisher
	Fischer R, Roux A, Wareham L, Sappington R. Pressure-dependent modulation of inward-rectifying K+ channels: implications for cation homeostasis and K+ dynamics in glaucoma. Am J Physiol Cell Physiol. 2019;317:C375-C389 pubmed publisher
	Guan Y, Gao X, Tang Q, Huang L, Gao S, Yu S, et al. Nucleoporin 107 facilitates the nuclear export of Scn5a mRNA to regulate cardiac bioelectricity. J Cell Mol Med. 2019;23:1448-1457 pubmed publisher
	Xia P, Li L, Qi R, Shi J, Ju W, Chen M. Electrical and histological remodeling of the pulmonary vein in 2K1C hypertensive rats: Indication of initiation and maintenance of atrial fibrillation. Anatol J Cardiol. 2018;19:169-175 pubmed publisher
	Sun Y, Timofeyev V, Dennis A, Bektik E, Wan X, Laurita K, et al. A Singular Role of IK1 Promoting the Development of Cardiac Automaticity during Cardiomyocyte Differentiation by IK1 -Induced Activation of Pacemaker Current. Stem Cell Rev. 2017;13:631-643 pubmed publisher
	Caballero R, Utrilla R, AmorÃ³s I, Matamoros M, PÃ©rez HernÃ¡ndez M, Tinaquero D, et al. Tbx20 controls the expression of the KCNH2 gene and of hERG channels. Proc Natl Acad Sci U S A. 2017;114:E416-E425 pubmed publisher
	Rubi L, Koenig X, Kubista H, Todt H, Hilber K. Decreased inward rectifier potassium current IK1 in dystrophin-deficient ventricular cardiomyocytes. Channels (Austin). 2017;11:101-108 pubmed publisher
	Li Q, Cui P, Miao Y, Gao F, Li X, Qian W, et al. Activation of group I metabotropic glutamate receptors regulates the excitability of rat retinal ganglion cells by suppressing Kir and I h. Brain Struct Funct. 2017;222:813-830 pubmed publisher
	Murata Y, Yasaka T, Takano M, Ishihara K. Neuronal and glial expression of inward rectifier potassium channel subunits Kir2.x in rat dorsal root ganglion and spinal cord. Neurosci Lett. 2016;617:59-65 pubmed publisher

image

image 1 :

Western blot analysis of rat heart (lanes 1 and 3) and rat brain (lanes 2 and 4) membranes: - 1,2. Anti-Kir2.1/KCNJ2 Antibody (#APC-026), (1:200).3,4. Anti-Kir2.1/KCNJ2 Antibody, preincubated with Kir2.1/KCNJ2 Blocking Peptide (#BLP-PC026).

image 2 :

Peptide (C)NGVPESTSTDTPPDIDLHN corresponding to amino acid residues 392-410 of human Kir2.1 (AccessionP48049). Intracellular C-terminus.

image 3 :

Peptide (C)NGVPESTSTDTPPDIDLHN corresponding to amino acid residues 392-410 of human Kir2.1 (AccessionP48049). Intracellular C-terminal part.

product information

CAT :

APC-026

SKU :

APC-026-CF_0.2 ml

Product Name :

Anti-Kir2.1/KCNJ2 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P48049

Applications :

IC IF IFC IHC IP WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PC026

Homology :

Rabbit, bovine, pig, guinea pig - identical; rat, mouse - 17/19 amino acid residues identical; chicken - 15/19 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminal domain

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

(C)NGVPESTSTDTPPDIDLHN, corresponding to amino acid residues 392-410 of human Kir2.1

Product Page - Scientific background :

Kir2.1 is a member of the family of inward rectifying K+ channels. The family includes 15 members that are structurally and functionally different from the voltage-dependent K+ channels.1 The family's topology consists of two transmembrane domains that flank a single and highly conserved pore region with intracellular N- and C-termini. As is the case for the voltage-dependent K+ channels the functional unit for the Kir channels is composed of four subunits that can assemble as either homo- or heterotetramers.Kir channels are characterized by a K+ efflux that is limited by depolarizing membrane potentials thus making them essential for controlling resting membrane potential and K+ homeostasis.Kir2.1 is a member of the Kir2.x subfamily that includes four members (Kir2.1- Kir2.4) that are characterized by strong inward rectification and high constitutive activity.Kir2.1 is expressed in a variety of tissues including the heart, brain, vascular smooth muscle cells and skeletal muscles.In the heart, Kir2.1 is a molecular component of the IK1 current that is responsible for setting the resting membrane potential, preventing membrane hyperpolarization due to Na+ pump activity, influencing propagation velocity, altering the electrical space constant, and promoting late phase repolarization.2 In fact, mutations in Kir2.1 channels have been linked to a form of long QT syndrome (LQT7) known as Andersen's syndrome that is characterized by cardiac arrhythmias, periodic paralysis, and dysmorphic features.3

Applications may also work in :

IC IF IFC IHC IP WB

Supplier :

Alomone Labs

Target :

Inward rectifier potassium channel 2, IRK1, HIRK1, LQT7, SQT3

Short Description :

A Rabbit Polyclonal Antibody to Kir2.1 (KCNJ2) Channel

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of human Kir2.1 channel. Anti-Kir2.1/KCNJ2 Antibody (#APC-026) can be used in western blot, immunoprecipitation, immunocytochemistry, immunohistochemistry, and flow cytometry applications. It has been designed to recognize Kir2.1 from human, rat, and mouse samples.

Negative Control :

BLP-PC026

Positive Control :

Synonyms :

Inward rectifier potassium channel 2, IRK1, HIRK1, LQT7, SQT3

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

KCNJ2

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IHC ICC IFC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage