Guinea pig Anti-Kir2.1/KCNJ2 Antibody | Alomone Labs APC-026-GP product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Guinea pig Anti-Kir2.1/KCNJ2 Antibody

catalog :

APC-026-GP

clonality :

polyclonal

host :

guinea-pigs

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot

more info or order :

Alomone Labs product webpage

citations: 3

Reference
Vila A, Shihabeddin E, Zhang Z, Santhanam A, Ribelayga C, O BRIEN J. Synaptic Scaffolds, Ion Channels and Polyamines in Mouse Photoreceptor Synapses: Anatomy of a Signaling Complex. Front Cell Neurosci. 2021;15:667046 pubmed publisher
Park S, Ponce Balbuena D, Kuick R, Guerrero Serna G, Yoon J, Mellacheruvu D, et al. Kir2.1 interactome mapping uncovers PKP4 as a modulator of the Kir2.1-regulated inward rectifier potassium currents. Mol Cell Proteomics. 2020;: pubmed publisher
Guan Y, Gao X, Tang Q, Huang L, Gao S, Yu S, et al. Nucleoporin 107 facilitates the nuclear export of Scn5a mRNA to regulate cardiac bioelectricity. J Cell Mol Med. 2019;23:1448-1457 pubmed publisher

image

image 1 :

Western blot analysis of mouse heart lysate (lanes 1 and 3) and rat brain membranes (lanes 2 and 4): - 1-2. Guinea pig Anti-Kir2.1/KCNJ2 Antibody (#APC-026-GP), (1:500).3-4. Guinea pig Anti-Kir2.1/KCNJ2 Antibody, preincubated with Kir2.1/KCNJ2 Blocking Peptide (#BLP-PC026).

product information

CAT :

APC-026-GP

SKU :

APC-026-GP-CF_0.2 ml

Product Name :

Guinea pig Anti-Kir2.1/KCNJ2 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P48049

Applications :

Reactivity :

Human Rat Mouse

Host :

Guinea Pig

Blocking Peptide :

BLP-PC026

Homology :

Rabbit, bovine, pig, guinea pig - identical; rat, mouse - 17/19 amino acid residues identical; chicken - 15/19 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Guinea pig total IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminal domain

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

(C)NGVPESTSTDTPPDIDLHN, corresponding to amino acid residues 392-410 of human Kir2.1

Product Page - Scientific background :

Kir2.1 is a member of the family of inward rectifying K+ channels. The family includes 15 members that are structurally and functionally different from the voltage-dependent K+ channels.1 The family's topology consists of two transmembrane domains that flank a single and highly conserved pore region with intracellular N- and C-termini. As is the case for the voltage-dependent K+ channels the functional unit for the Kir channels is composed of four subunits that can assemble as either homo- or heterotetramers.Kir channels are characterized by a K+ efflux that is limited by depolarizing membrane potentials thus making them essential for controlling resting membrane potential and K+ homeostasis.Kir2.1 is a member of the Kir2.x subfamily that includes four members (Kir2.1- Kir2.4) that are characterized by strong inward rectification and high constitutive activity.Kir2.1 is expressed in a variety of tissues including the heart, brain, vascular smooth muscle cells and skeletal muscles.In the heart, Kir2.1 is a molecular component of the IK1 current that is responsible for setting the resting membrane potential, preventing membrane hyperpolarization due to Na+ pump activity, influencing propagation velocity, altering the electrical space constant, and promoting late phase repolarization.2 In fact, mutations in Kir2.1 channels have been linked to a form of long QT syndrome (LQT7) known as Andersen's syndrome that is characterized by cardiac arrhythmias, periodic paralysis, and dysmorphic features.3

Applications may also work in :

Supplier :

Alomone Labs

Target :

Inward rectifier potassium channel 2, IRK1, HIRK1, LQT7, SQT3

Short Description :

A Guinea Pig Polyclonal Antibody to Kir2.1 (KCNJ2) Channel

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of the human Kir2.1 channel. Guinea pig Anti-Kir2.1/KCNJ2 Antibody (#APC-026-GP, formerly AGP-044) raised in guinea pig can be used in western blot analysis. It was designed to recognize Kir2.1 from human, rat and mouse samples. The antigen used to immunize guinea pigs is the same as Anti-Kir2.1/KCNJ2 Antibody (#APC-026) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation, etc.

Negative Control :

BLP-PC026

Positive Control :

Synonyms :

Inward rectifier potassium channel 2, IRK1, HIRK1, LQT7, SQT3

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

KCNJ2

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

ICC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:500

more info or order :

Alomone Labs product webpage