Anti-KCNN3 (KCa2.3, SK3) (N-term) Antibody | Alomone Labs APC-025 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-KCNN3 (KCa2.3, SK3) (N-term) Antibody

catalog :

APC-025

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - frozen section

more info or order :

Alomone Labs product webpage

citations: 21

Published Application/Species/Sample/Dilution	Reference
western blot; mouse; 1:500; loading ...; fig 6a	Deng P, Carlin D, Oh Y, Myrick L, Warren S, Cavalli V, et al. Voltage-Independent SK-Channel Dysfunction Causes Neuronal Hyperexcitability in the Hippocampus of Fmr1 Knock-Out Mice. J Neurosci. 2019;39:28-43 pubmed publisher
immunohistochemistry - frozen section; rat; 1:1000; loading ...; fig 4e immunocytochemistry; rat; 1:1000; loading ...; fig 1	Jager S, Pallesen L, Vaegter C. Isolation of satellite glial cells for high-quality RNA purification. J Neurosci Methods. 2018;297:1-8 pubmed publisher
immunoprecipitation; rat; fig 2e western blot; rat; 1:1000; fig 2e	Pizzoni A, López González M, Di Giusto G, Rivarola V, Capurro C, Ford P. AQP2 can modulate the pattern of Ca2+ transients induced by store-operated Ca2+ entry under TRPV4 activation. J Cell Biochem. 2018;119:4120-4133 pubmed publisher
western blot; rat; loading ...; fig 3a	Kim T, Terentyeva R, Roder K, Li W, Liu M, Greener I, et al. SK channel enhancers attenuate Ca2+-dependent arrhythmia in hypertrophic hearts by regulating mito-ROS-dependent oxidation and activity of RyR. Cardiovasc Res. 2017;113:343-353 pubmed publisher
immunohistochemistry - frozen section; mouse; 1:200; fig 5 immunocytochemistry; mouse; 1:100; fig 3 western blot; mouse; 1:1000; fig 1	Li Y, Hu H, Butterworth M, Tian J, Zhu M, O Neil R. Expression of a Diverse Array of Ca2+-Activated K+ Channels (SK1/3, IK1, BK) that Functionally Couple to the Mechanosensitive TRPV4 Channel in the Collecting Duct System of Kidney. PLoS ONE. 2016;11:e0155006 pubmed publisher
	Tiffner A, Hopl V, Schober R, Sallinger M, Grabmayr H, Höglinger C, et al. Orai1 Boosts SK3 Channel Activation. Cancers (Basel). 2021;13: pubmed publisher
	Lee K, Park S, Choi P, Ryoo S, Lee H, Peri L, et al. Molecular and functional characterization of detrusor PDGFRα positive cells in spinal cord injury-induced detrusor overactivity. Sci Rep. 2021;11:16268 pubmed publisher
	Rahm A, Gramlich D, Wieder T, Müller M, Schoeffel A, El Tahry F, et al. Trigger-Specific Remodeling of KCa2 Potassium Channels in Models of Atrial Fibrillation. Pharmgenomics Pers Med. 2021;14:579-590 pubmed publisher
	Pinilla E, S xe1 nchez A, Mart xed nez M, Mu xf1 oz M, Garc xed a Sacrist xe1 n A, K xf6 hler R, et al. Endothelial KCa 1.1 and KCa 3.1 channels mediate rat intrarenal artery endothelium-derived hyperpolarization response. Acta Physiol (Oxf). 2021;231:e13598 pubmed publisher
	Huang J, Zhang H, Tan X, Hu M, Shen B. Exercise restores impaired endothelium-derived hyperpolarizing factor-mediated vasodilation in aged rat aortic arteries via the TRPV4-KCa2.3 signaling complex. Clin Interv Aging. 2019;14:1579-1587 pubmed publisher
	Qu L, Wang Y, Ge S, Li N, Fu J, Zhang Y, et al. Altered Activity of SK Channel Underpins Morphine Withdrawal Relevant Psychiatric Deficiency in Infralimbic to Accumbens Shell Pathway. Front Psychiatry. 2019;10:240 pubmed publisher
	Ling T, Yi F, Lu T, Wang X, Sun X, Willis M, et al. F-box protein-32 down-regulates small-conductance calcium-activated potassium channel 2 in diabetic mouse atria. J Biol Chem. 2019;294:4160-4168 pubmed publisher
	Fan X, Yu Y, Lan H, Ou X, Yang L, Li T, et al. Ca2+/Calmodulin-Dependent Protein Kinase II (CaMKII) Increases Small-Conductance Ca2+-Activated K+ Current in Patients with Chronic Atrial Fibrillation. Med Sci Monit. 2018;24:3011-3023 pubmed publisher
	Hartmann S, Zheng F, Kyncl M, Karch S, Voelkl K, Zott B, et al. Î²-Secretase BACE1 Promotes Surface Expression and Function of Kv3.4 at Hippocampal Mossy Fiber Synapses. J Neurosci. 2018;38:3480-3494 pubmed publisher
	Liu Y, Cole V, Lawandy I, Ehsan A, Sellke F, Feng J. Decreased coronary arteriolar response to KCa channel opener after cardioplegic arrest in diabetic patients. Mol Cell Biochem. 2018;445:187-194 pubmed publisher
	Lee H, Koh B, Peri L, Corrigan R, Lee H, George N, et al. Premature contractions of the bladder are suppressed by interactions between TRPV4 and SK3 channels in murine detrusor PDGFRα+ cells. Sci Rep. 2017;7:12245 pubmed publisher
	Lu T, Wang X, Chai Q, Sun X, Sieck G, Katusic Z, et al. Role of the endothelial caveolae microdomain in shear stress-mediated coronary vasorelaxation. J Biol Chem. 2017;292:19013-19023 pubmed publisher
	He D, Pan Q, Chen Z, Sun C, Zhang P, Mao A, et al. Treatment of hypertension by increasing impaired endothelial TRPV4-KCa2.3 interaction. EMBO Mol Med. 2017;9:1491-1503 pubmed publisher
	Zhang Y, Zhang Z, Xiao S, Tien J, Le S, Le T, et al. Inferior Olivary TMEM16B Mediates Cerebellar Motor Learning. Neuron. 2017;95:1103-1111.e4 pubmed publisher
	Torrente A, Zhang R, Wang H, Zaini A, Kim B, Yue X, et al. Contribution of small conductance K+ channels to sinoatrial node pacemaker activity: insights from atrial-specific Na+ /Ca2+ exchange knockout mice. J Physiol. 2017;595:3847-3865 pubmed publisher
	Yap F, Weber D, Taylor M, Townsley M, Comer B, Maylie J, et al. Endothelial SK3 channel-associated Ca2+ microdomains modulate blood pressure. Am J Physiol Heart Circ Physiol. 2016;310:H1151-63 pubmed publisher

image

image 1 :

Western blot analysis of rat brain membranes: - 1. Anti-KCNN3 (KCa2.3, SK3) (N-term) Antibody (#APC-025), (1:200).2. Anti-KCNN3 (KCa2.3, SK3) (N-term) Antibody, preincubated with KCNN3/KCa2.3 (N-term) Blocking Peptide (#BLP-PC025).

image 2 :

Expression of KCa2.3 (SK3) in mouse dopaminergic neurons - Immunohistochemical staining of mouse dopaminergic neurons using Anti-KCNN3 (KCa2.3, SK3) (N-term) Antibody (#APC-025). A. KCa2.3 is detected in substantia nigra pars compacta. B. Tyrosine hydroxylase staining shows dopaminergic neurons. Triangles point at cells with co-localization.

product information

CAT :

APC-025

SKU :

APC-025-CF_0.2 ml

Product Name :

Anti-KCNN3 (KCa2.3, SK3) (N-term) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q9UGI6

Applications :

IC IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PC025

Homology :

Rat, mouse, pig - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

DTSGHFHDSGVGDLDEDPKC, corresponding to amino acid residues 2-21 of human KCNN3

Product Page - Scientific background :

KCa2.3 (KCNN3, SK3) is a member of the Ca2+-activated K+ channel family with small conductance that includes KCa2.1 (KCNN1, SK1) and KCa2.2 (KCNN2, SK2). The channel is voltage insensitive and is activated by intracellular Ca2+ in the submicromolar range. It has, though, a similar topology to that of voltage-dependent K+ channels (KV channels), that is six transmembrane domains and intracellular N- and C-termini. The functional channel of all the KCa2 family members is a multimeric protein composed of four pore-forming subunits.KCa2 channels are extremely sensitive to the levels of intracellular Ca2+ and concentrations as low as 300-700 nM can open the channels very rapidly (5-15 ms). Hence, the KCa2 channels are highly sensitive and fast Ca2+ sensors resembling other known Ca2+-binding proteins. This type of Ca2+-dependent activation is achieved by the constitutive binding of the KCa2 channels to calmodulin, a highly expressed Ca2+-binding protein via a calmodulin-binding domain situated at the cytoplasmic C-terminus.Pharmacologically, the KCa2 channels are the only known targets of the bee venom toxin Apamin, with KCa2.1 being the least sensitive, KCa2.2 the most sensitive and KCa2.3 showing intermediate sensitivity.KCa2.3 is predominantly expressed in the nervous system although expression in endothelial cells, heart and liver have been described.KCa2.3 is known to be involved in the regulation of neuronal excitability. They do so mainly via a phenomenon known as after hyperpolarization in which KCa2 channels open in response to increased intracellular Ca2+ concentrations that result from the entry of extracellular Ca2+ through voltage-dependent Ca2+ channels during action potentials. In this way, KCa2 channels effectively form a Ca2+-mediated feedback loop.KCa2.3 is involved in the control of firing rate and subsequent dopamine secretion from midbrain dopaminergic neurons. Since malfunction of these neurons is involved in several pathological disorders such as Parkinson's disease and Schizophrenia, modulators of the KCa2.3 channels have been proposed to be of therapeutic value in these diseases.

Applications may also work in :

IC IF IHC WB

Supplier :

Alomone Labs

Target :

Small conductance calcium-activated potassium channel protein 3, SKCa3

Short Description :

A Rabbit Polyclonal Antibody to KCNN3 (KCa2.3, SK3) Channel

Long Description :

Anti-KCNN3 (KCa2.3, SK3) (N-term) Antibody (#APC-025) is a highly specific antibody directed against an intracellular epitope at the N-terminus of the human KCNN3 channel. The antibody can be used in western blot, immunocytochemistry, and immunohistochemistry applications. It has been designed to recognize KCNN3 from human, rat, and mouse samples.

Negative Control :

BLP-PC025

Positive Control :

Synonyms :

Small conductance calcium-activated potassium channel protein 3, SKCa3

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

KCNN3

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

KO-Validated :

yes

Cited Application :

IP IHC ICC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage