antibody

Anti-KCNN3 (KCa2.3, SK3) (N-term)-ATTO Fluor-594 Antibody

APC-025-AR

polyclonal

domestic rabbit

ATTO 594

NA

human, mouse, rat

immunohistochemistry, immunocytochemistry, flow cytometry

APC-025-AR_50 mcl

Anti-KCNN3 (KCa2.3, SK3) (N-term)-ATTO Fluor-594 Antibody

Antibodies

Antibodies

Polyclonal

Q9UGI6

FC IC IF IHC

Human Rat Mouse

Rabbit

BLP-PC025

Rat, mouse, pig - identical

PBS pH7.4, 1% BSA with 0.05% sodium azide

Rabbit IgG

Confirmed by amino acid analysis and mass spectrometry

50 µl double distilled water (DDW).

1 mg/ml

The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

1% BSA, 0.05% NaN3

Intracellular, N-terminus

ATTO-594. Maximum absorption 601 nm; Maximum fluorescence 627 nm. The fluorescence is excited most efficiently in the 580 - 615 nm range. This label belongs to the class of Rhodamine dyes and can be used with fluorescent equipment typically optimized to detect Texas Red and Alexa-594.

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

Human

DTSGHFHDSGVGDLDEDPKC, corresponding to amino acid residues 2-21 of human KCNN3

KCa2.3 (KCNN3, SK3) is a member of the Ca2+-activated K+ channel family with small conductance that includes KCa2.1 (KCNN1, SK1) and KCa2.2 (KCNN2, SK2). The channel is voltage insensitive and is activated by intracellular Ca2+ in the submicromolar range. It has, though, a similar topology to that of voltage-dependent K+ channels (KV channels), that is six transmembrane domains and intracellular N- and C-termini. The functional channel of all the KCa2 family members is a multimeric protein composed of four pore-forming subunits.KCa2 channels are extremely sensitive to the levels of intracellular Ca2+ and concentrations as low as 300-700 nM can open the channels very rapidly (5-15 ms). Hence, the KCa2 channels are highly sensitive and fast Ca2+ sensors resembling other known Ca2+-binding proteins. This type of Ca2+-dependent activation is achieved by the constitutive binding of the KCa2 channels to calmodulin, a highly expressed Ca2+-binding protein via a calmodulin-binding domain situated at the cytoplasmic C-terminus.Pharmacologically, the KCa2 channels are the only known targets of the bee venom toxin Apamin, with KCa2.1 being the least sensitive, KCa2.2 the most sensitive and KCa2.3 showing intermediate sensitivity.KCa2.3 is predominantly expressed in the nervous system although expression in endothelial cells, heart and liver have been described.KCa2.3 is known to be involved in the regulation of neuronal excitability. They do so mainly via a phenomenon known as after hyperpolarization in which KCa2 channels open in response to increased intracellular Ca2+ concentrations that result from the entry of extracellular Ca2+ through voltage-dependent Ca2+ channels during action potentials. In this way, KCa2 channels effectively form a Ca2+-mediated feedback loop.KCa2.3 is involved in the control of firing rate and subsequent dopamine secretion from midbrain dopaminergic neurons. Since malfunction of these neurons is involved in several pathological disorders such as Parkinson's disease and Schizophrenia, modulators of the KCa2.3 channels have been proposed to be of therapeutic value in these diseases.

FC IC IF IHC

Alomone Labs

Small conductance calcium-activated potassium channel protein 3, SKCa3

A Rabbit Polyclonal Antibody to KCNN3 (KCa2.3, SK3) Channel Conjugated to the Fluorescent Dye ATTO-594

Anti-KCNN3 (KCa2.3, SK3) (N-term) Antibody (#APC-025) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot, immunocytochemistry, and immunohistochemistry applications. It has been designed to recognize KCNN3 from human, rat, and mouse samples. Anti-KCNN3 (KCa2.3, SK3) (N-term)-ATTO Fluor-594 Antibody (#APC-025-AR) is directly labeled with an ATTO-594 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The ATTO-594 fluorescent label belongs to the class of Rhodamine dyes and can be used with fluorescent equipment typically optimized to detect Texas Red and Alexa-594. Anti-KCNN3 (KCa2.3, SK3) (N-term)-ATTO Fluor-594 Antibody is specially suited to experiments requiring simultaneous labeling of different markers.

BLP-PC025

NA

Small conductance calcium-activated potassium channel protein 3, SKCa3

1-2 Business Days

Israel/IL

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

KCNN3

Lyophilized powder

Contact Alomone Labs for technical support and product customization

H- Human, M- Mouse, R- Rat

No

Affinity purified on immobilized antigen.

41116161

yes

ICC FC

NA

Western blot analysis (unlabeled antibody, #APC-025), and immunohistochemistry (labeled antibody).

1 µg peptide per 1 µg antibody

Contact Alomone for information

Contact Alomone for information