Anti-KCNK3 (TASK-1) Antibody | Alomone Labs APC-024 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-KCNK3 (TASK-1) Antibody

catalog :

APC-024

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

Alomone Labs product webpage

citations: 16

Published Application/Species/Sample/Dilution	Reference
western blot; human; 1:100; fig 1c	Suzuki Y, Tsutsumi K, Miyamoto T, Yamamura H, Imaizumi Y. Heterodimerization of two pore domain K+ channel TASK1 and TALK2 in living heterologous expression systems. PLoS ONE. 2017;12:e0186252 pubmed publisher
immunocytochemistry; mouse; fig 5 western blot; mouse; fig 5	Unudurthi S, Wu X, Qian L, Amari F, Onal B, Li N, et al. Two-Pore K+ Channel TREK-1 Regulates Sinoatrial Node Membrane Excitability. J Am Heart Assoc. 2016;5:e002865 pubmed publisher
	Han L, Song N, Hu X, Zhu A, Wei X, Liu J, et al. Inhibition of RELM-β prevents hypoxia-induced overproliferation of human pulmonary artery smooth muscle cells by reversing PLC-mediated KCNK3 decline. Life Sci. 2020;246:117419 pubmed publisher
	Garc a Morales V, Rodr guez Bey G, G mez P rez L, Dom nguez V as G, Gonz lez Forero D, Portillo F, et al. Sp1-regulated expression of p11 contributes to motor neuron degeneration by membrane insertion of TASK1. Nat Commun. 2019;10:3784 pubmed publisher
	Fischer R, Roux A, Wareham L, Sappington R. Pressure-dependent modulation of inward-rectifying K+ channels: implications for cation homeostasis and K+ dynamics in glaucoma. Am J Physiol Cell Physiol. 2019;317:C375-C389 pubmed publisher
	Yarishkin O, Phuong T, Bretz C, Olsen K, Baumann J, Lakk M, et al. TREK-1 channels regulate pressure sensitivity and calcium signaling in trabecular meshwork cells. J Gen Physiol. 2018;150:1660-1675 pubmed publisher
	Denton C, Price A, Friend J, Manithody C, Blomenkamp K, Westrich M, et al. Role of the Gut⁻Liver Axis in Driving Parenteral Nutrition-Associated Injury. Children (Basel). 2018;5: pubmed publisher
	Wang X, Guan R, Zhao X, Zhu D, Song N, Shen L. TASK1 and TASK3 Are Coexpressed With ASIC1 in the Ventrolateral Medulla and Contribute to Central Chemoreception in Rats. Front Cell Neurosci. 2018;12:285 pubmed publisher
	Yuan F, Wang H, Feng J, Wei Z, Yu H, Zhang X, et al. Leptin Signaling in the Carotid Body Regulates a Hypoxic Ventilatory Response Through Altering TASK Channel Expression. Front Physiol. 2018;9:249 pubmed publisher
	Srisomboon Y, Zaidman N, Maniak P, Deachapunya C, O Grady S. P2Y receptor regulation of K2P channels that facilitate K+ secretion by human mammary epithelial cells. Am J Physiol Cell Physiol. 2018;314:C627-C639 pubmed publisher
	Na B, Zhang H, Wang G, Dai L, Xia G. The Effect of Mechanical Ventilation on TASK-1 Expression in the Brain in a Rat Model. Can Respir J. 2017;2017:8530352 pubmed publisher
	Murtaza G, Mermer P, Goldenberg A, Pfeil U, Paddenberg R, Weissmann N, et al. TASK-1 potassium channel is not critically involved in mediating hypoxic pulmonary vasoconstriction of murine intra-pulmonary arteries. PLoS ONE. 2017;12:e0174071 pubmed publisher
	Puissant M, Mouradian G, Liu P, Hodges M. Identifying Candidate Genes that Underlie Cellular pH Sensitivity in Serotonin Neurons Using Transcriptomics: A Potential Role for Kir5.1 Channels. Front Cell Neurosci. 2017;11:34 pubmed publisher
	Telles C, Decker S, Motley W, Peters A, Mehr A, Frizzell R, et al. Functional and molecular identification of a TASK-1 potassium channel regulating chloride secretion through CFTR channels in the shark rectal gland: implications for cystic fibrosis. Am J Physiol Cell Physiol. 2016;311:C884-C894 pubmed publisher
	Leithner K, Hirschmugl B, Li Y, Tang B, Papp R, Nagaraj C, et al. TASK-1 Regulates Apoptosis and Proliferation in a Subset of Non-Small Cell Lung Cancers. PLoS ONE. 2016;11:e0157453 pubmed publisher
	Shinoda K, Luijten I, Hasegawa Y, Hong H, Sonne S, Kim M, et al. Genetic and functional characterization of clonally derived adult human brown adipocytes. Nat Med. 2015;21:389-94 pubmed publisher

image

image 1 :

Western blot analysis of rat brain (1, 3) and pancreas (2, 4) membranes: - 1,2. Anti-KCNK3 (TASK-1) Antibody (#APC-024), (1:200).3,4. Anti-KCNK3 (TASK-1) Antibody, preincubated with KCNK3/TASK-1 Blocking Peptide (#BLP-PC024).

image 2 :

Western blot analysisof rat brain (1 3) and pancreas (2 4) membranes: - 12.Anti-KCNK3(TASK-1)Antibody(#APC-024) (1:200).34. Anti-KCNK3(TASK-1) Antibody preincubated with the negative control antigen.

product information

CAT :

APC-024

SKU :

APC-024-CF_0.2 ml

Product Name :

Anti-KCNK3 (TASK-1) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

O14649

Applications :

IC IF IHC IP WB

Reactivity :

Human Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PC024

Homology :

Rat, mouse - 17/18 amino acid residues identical Homology with human TASK-3: 11/17 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminal domain

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

(C)EDEKRDAEHRALLTRNGQ, corresponding to amino acid residues 252-269 of human KCNK3

Product Page - Scientific background :

KCNK3 (also named TWIK-related acid sensitive K+ channel, TASK-1 or K2P3.1) is a member of the 2-pore (2P) domain K+ channels family that includes at least 16 members. These channels show little time- or voltage-dependence and are considered to be "leak" or "background" K+ channels, thereby generating background currents which help set the membrane resting potential and cell excitation.The K2P channels have a signature topology that includes four transmembrane domains and two pore domains with intracellular N- and C- termini. The functional channel is believed to be a dimer.K2P channels are regulated by diverse physical and chemical stimuli including temperature, changes in intracellular pH, mechanical stretch, inhalation anesthetics, etc. The channels can then be subclassified based in their specific activators.KCNK3 is extremely sensitive to variations in external pH, a drop in pH from 7.7 to 6.7 will be enough to close most KCNK3 channels.KCNK3 channel distribution is relatively wide with high expression in the brain and heart as well as in the pancreas, kidney and lung.KCNK3 can exist in the brain either as a homodimer or as a heterodimer with the closely related channel K2P9.1 (TASK-3).During periods of high neuronal activity a transient increase in extracellular pH has been recorded. This pH increase may stimulate KCNK3 channel activity and hence enhance outward K+ currents. This in turn will decrease excitability in these neurons and work as a feedback mechanism.

Applications may also work in :

IC IF IHC IP WB

Supplier :

Alomone Labs

Target :

Potassium channel subfamily K member 3, TWIK-related acid sensitive K+ channel, K2P3.1, cTBAK-1, OAT1

Short Description :

A Rabbit Polyclonal Antibody to KCNK3 Channel

Long Description :

Anti-KCNK3 (TASK-1) Antibody (#APC-024) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot, immunohistochemistry, immunocytochemistry, and immunoprecipitation applications. It has been designed to recognize TASK-1 channel from mouse, rat, and human samples.

Negative Control :

BLP-PC024

Positive Control :

Synonyms :

Potassium channel subfamily K member 3, TWIK-related acid sensitive K+ channel, K2P3.1, cTBAK-1, OAT1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

KCNK3

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IP IHC ICC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage