Anti-KV4.2 Antibody | Alomone Labs APC-023 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-KV4.2 Antibody

catalog :

APC-023

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

Alomone Labs product webpage

citations: 10

Reference
Kim K, Jeong H, Kim Y, Lee S, Kim Y, Kim H, et al. Calbindin regulates Kv4.1 trafficking and excitability in dentate granule cells via CaMKII-dependent phosphorylation. Exp Mol Med. 2021;53:1134-1147 pubmed publisher
Pluteanu F, Seidl M, Hamer S, Scholz B, Müller F. Inward Rectifier K+ Currents Contribute to the Proarrhythmic Electrical Phenotype of Atria Overexpressing Cyclic Adenosine Monophosphate Response Element Modulator Isoform CREM-IbΔC-X. J Am Heart Assoc. 2020;9:e016144 pubmed publisher
S xe1 nchez Aguilera A, Monedero G, Colino A, Vicente Torres M. Development of Action Potential Waveform in Hippocampal CA1 Pyramidal Neurons. Neuroscience. 2020;442:151-167 pubmed publisher
Kim K, Lee S, Yoon S, Kim Y, Jeong H, Lee S, et al. Kv4.1, a Key Ion Channel For Low Frequency Firing of Dentate Granule Cells, Is Crucial for Pattern Separation. J Neurosci. 2020;40:2200-2214 pubmed publisher
Carll A, Salatini R, Pirela S, Wang Y, Xie Z, Lorkiewicz P, et al. Inhalation of printer-emitted particles impairs cardiac conduction, hemodynamics, and autonomic regulation and induces arrhythmia and electrical remodeling in rats. Part Fibre Toxicol. 2020;17:7 pubmed publisher
Welch M, Forster L, Atlas S, Baro D. SUMOylating Two Distinct Sites on the A-type Potassium Channel, Kv4.2, Increases Surface Expression and Decreases Current Amplitude. Front Mol Neurosci. 2019;12:144 pubmed publisher
Alfaro Ruiz R, Aguado C, Martín Belmonte A, Moreno Martínez A, Lujan R. Expression, Cellular and Subcellular Localisation of Kv4.2 and Kv4.3 Channels in the Rodent Hippocampus. Int J Mol Sci. 2019;20: pubmed publisher
Day S, Yang W, Ewin S, Zhou X, Ma T. Glucagon-like peptide-1 cleavage product GLP-1 (9-36) amide enhances hippocampal long-term synaptic plasticity in correlation with suppression of Kv4.2 expression and eEF2 phosphorylation. Hippocampus. 2017;27:1264-1274 pubmed publisher
Liu X, Zhang Y, Du W, Liang H, He H, Zhang L, et al. MiR-223-3p as a Novel MicroRNA Regulator of Expression of Voltage-Gated K+ Channel Kv4.2 in Acute Myocardial Infarction. Cell Physiol Biochem. 2016;39:102-14 pubmed publisher
Wolff M, Czorlich P, Nagaraj C, SchnÃ¶bel Ehehalt R, Li Y, Kwapiszewska G, et al. Amitriptyline and carbamazepine utilize voltage-gated ion channel suppression to impair excitability of sensory dorsal horn neurons in thin tissue slice: An in vitro study. Neurosci Res. 2016;109:16-27 pubmed publisher

image

image 1 :

Western blot analysis of rat brain membranes: - 1. Anti-KV4.2 Antibody (#APC-023), (1:200).2. Anti-KV4.2 Antibody, preincubated with Kv4.2 Blocking Peptide (#BLP-PC023).

image 2 :

Peptide (C)SNQLQSSEDEPAFVSK corresponding to amino acid residues 454-469 of rat KV4.2 (AccessionQ63881). Intracellular C-terminus.

image 3 :

Alomone Labs A-794278inhibits mGluR1-mediated Ca2+mobilization in U2OS cells. - Dose-response ofA-794278(#A-435) normalized inhibition of human mGluR1-mediated L-glutamate-evoked Ca2+mobilization. Cells were loaded with calcium-sensitive dye incubated with a range of A-794278 concentrations and stimulated with 5 M L-glutamate (EC80). Changes in intracellular Ca2+following stimulation were detected as changes in maximum relative fluorescence (RLU) using FLIPRTETRA?. IC50was determined at 4.16 nM.

product information

CAT :

APC-023

SKU :

APC-023-CF_0.2 ml

Product Name :

Anti-KV4.2 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q63881

Applications :

IC IF IHC IP WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PC023

Homology :

Mouse, human - 15/16 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)SNQLQSSEDEPAFVSK, corresponding to amino acid residues 454-469 of rat KV4.2

Product Page - Scientific background :

KV4.2 is a voltage-dependent K+ channel that belongs to the Shal channel subfamily and includes two other members: KV4.1 and KV4.3.1KV4.2 possesses the signature structure of the voltage-dependent K+ channels: six membrane-spanning domains with intracellular N and C termini. As with other members of the voltage-gated K+ channel superfamily, the functional channel is a tetramer that can be composed of more than one member of the Shal subfamily, i.e. heterotetramers of KV4.1 and KV4.3.The KV4 channels are characterized by activation at subthreshold membrane potentials, inactivate rapidly and recover from inactivation quickly compared with other voltage-dependent K+ channels. This type of current is known as transient A-type K+ currents. For example, depolarization-activated K+ currents in rat neostriatal cholinergic interneurons are predominantly of the A-type and attributable to coexpression of KV4.2 and KV4.1 subunits.2The biophysical properties of the KV4.2 subunit can be modified by its association with auxiliary β subunits such as the KChIP family that increase KV4.2 current densities and accelerates both the inactivation and the recovery time.KV4.2 is also highly expressed in the heart where together with KV4.3 underlie the fast inactivating and recovering cardiac transient outward current Ito.3Several toxins from spider venoms are potent blockers (affecting the channels in the nanomolar range) of KV4.2 channels. Among these the most potent and selective are Stromatoxin-1 (#STS-350), (1.2nM), Phrixotoxin-1 (#STP-700), (5 nM), Phrixotoxin-2 (#STP-710), (34 nM) and Heteropodatoxin-2 (#STH-340), (100 nM).4

Applications may also work in :

IC IF IHC IP WB

Supplier :

Alomone Labs

Target :

Voltage-gated potassium channel subfamily D member 2, KCND2, Shal1, RK5

Short Description :

A Rabbit Polyclonal Antibody to KV4.2 Channel

Long Description :

Anti-KV4.2 Antibody (#APC-023) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunoprecipitation, immunocytochemistry, and immunohistochemistry applications. It has been designed to recognize KV4.2 from human, rat, and mouse samples.

Negative Control :

BLP-PC023

Positive Control :

Synonyms :

Voltage-gated potassium channel subfamily D member 2, KCND2, Shal1, RK5

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

KCND2

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IP IHC ICC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage