antibody

Anti-KV4.2-ATTO Fluor-633 Antibody

APC-023-FR

polyclonal

domestic rabbit

nonconjugated

NA

mouse, rat

immunohistochemistry, immunocytochemistry

APC-023-FR_50 mcl

Anti-KV4.2-ATTO Fluor-633 Antibody

Antibodies

Antibodies

Polyclonal

Q63881

IF IHC

Rat Mouse

Rabbit

BLP-PC023

Mouse, human - 15/16 amino acid residues identical

PBS pH7.4, 1% BSA with 0.05% sodium azide

Rabbit IgG

Confirmed by amino acid analysis and mass spectrometry

50 µl double distilled water (DDW).

1 mg/ml

The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

1% BSA, 0.05% NaN3

Intracellular, C-terminus

ATTO-633. Maximum absorption 629 nm; maximum fluorescence 657 nm. The fluorescence is excited most efficiently in the 610 - 645 nm range. This label is analogous to the dyes Alexa 647, Alexa 633 and Cy5 and can be used for direct flow cytometry (FACS) using the He:Ne laser.

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

Rat

(C)SNQLQSSEDEPAFVSK, corresponding to amino acid residues 454-469 of rat KV4.2

KV4.2 is a voltage-dependent K+ channel that belongs to the Shal channel subfamily and includes two other members: KV4.1 and KV4.3.1KV4.2 possesses the signature structure of the voltage-dependent K+ channels: six membrane-spanning domains with intracellular N and C termini. As with other members of the voltage-gated K+ channel superfamily, the functional channel is a tetramer that can be composed of more than one member of the Shal subfamily, i.e. heterotetramers of KV4.1 and KV4.3.The KV4 channels are characterized by activation at subthreshold membrane potentials, inactivate rapidly and recover from inactivation quickly compared with other voltage-dependent K+ channels. This type of current is known as transient A-type K+ currents. For example, depolarization-activated K+ currents in rat neostriatal cholinergic interneurons are predominantly of the A-type and attributable to coexpression of KV4.2 and KV4.1 subunits.2The biophysical properties of the KV4.2 subunit can be modified by its association with auxiliary β subunits such as the KChIP family that increase KV4.2 current densities and accelerates both the inactivation and the recovery time.KV4.2 is also highly expressed in the heart where together with KV4.3 underlie the fast inactivating and recovering cardiac transient outward current Ito.3Several toxins from spider venoms are potent blockers (affecting the channels in the nanomolar range) of KV4.2 channels. Among these the most potent and selective are Stromatoxin-1 (#STS-350), (1.2nM), Phrixotoxin-1 (#STP-700), (5 nM), Phrixotoxin-2 (#STP-710), (34 nM) and Heteropodatoxin-2 (#STH-340), (100 nM).4

IF IHC

Alomone Labs

Voltage-gated potassium channel subfamily D member 2, KCND2, Shal1, RK5

A Rabbit Polyclonal Antibody to KV4.2 Channel Conjugated to the Fluorescent Dye ATTO-633

Anti-KV4.2 Antibody (#APC-023) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunoprecipitation, immunocytochemistry, and immunohistochemistry applications. It has been designed to recognize KV4.2 from human, rat, and mouse samples. Anti-KV4.2-ATTO Fluor-633 Antibody (#APC-023-FR) is directly labeled with an ATTO-633 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. ATTO 633 has a maximum absorption at 629 nm and a maximum fluorescence at 657 nm. The fluorescence is excited most efficiently in the range 610 to 645 nm. This label is analogous to the well-known dyes Alexa 647, Alexa 633 and Cy5. Anti-KV4.2-ATTO Fluor-633 Antibody is especially suited for experiments requiring simultaneous labeling of different markers.

RIC-001-FR

NA

Voltage-gated potassium channel subfamily D member 2, KCND2, Shal1, RK5

1-2 Business Days

Israel/IL

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

KCND2

Lyophilized powder

Contact Alomone Labs for technical support and product customization

H- Human, M- Mouse, R- Rat

No

Affinity purified on immobilized antigen.

41116161

NA

Western blot analysis (unlabeled antibody, #APC-023), and immunohistochemistry (labeled antibody).

1 µg peptide per 1 µg antibody

1:60

Contact Alomone for information