Anti-KCNQ1 Antibody | Alomone Labs APC-022 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-KCNQ1 Antibody

catalog :

APC-022

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - frozen section

more info or order :

Alomone Labs product webpage

citations: 18

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry - frozen section; human; 1:100; fig 8	Liu W, Edin F, Blom H, Magnusson P, Schrott Fischer A, Glueckert R, et al. Super-resolution structured illumination fluorescence microscopy of the lateral wall of the cochlea: the Connexin26/30 proteins are separately expressed in man. Cell Tissue Res. 2016;365:13-27 pubmed publisher
	Song L, Bekdash R, Morikawa K, Quejada J, Klein A, Aina Badejo D, et al. Sigma non-opioid receptor 1 is a potential therapeutic target for long QT syndrome. Nat Cardiovasc Res. 2022;1:142-156 pubmed publisher
	Kabakov A, Sengun E, Lu Y, Roder K, Bronk P, Baggett B, et al. Three-Week-Old Rabbit Ventricular Cardiomyocytes as a Novel System to Study Cardiac Excitation and EC Coupling. Front Physiol. 2021;12:672360 pubmed publisher
	Gou X, Hu T, Gou Y, Li C, Yi M, Jia M. Specific protein kinase C isoform exerts chronic inhibition on the slowly activating delayed-rectifier potassium current by affecting channel trafficking. Channels (Austin). 2021;15:262-272 pubmed publisher
	Carll A, Salatini R, Pirela S, Wang Y, Xie Z, Lorkiewicz P, et al. Inhalation of printer-emitted particles impairs cardiac conduction, hemodynamics, and autonomic regulation and induces arrhythmia and electrical remodeling in rats. Part Fibre Toxicol. 2020;17:7 pubmed publisher
	Tian C, Johnson K. TBX1 is required for normal stria vascularis and semicircular canal development. Dev Biol. 2020;457:91-103 pubmed publisher
	Inagaki A, Hayashi M, Andharia N, Matsuda H. Involvement of butyrate in electrogenic K+ secretion in rat rectal colon. Pflugers Arch. 2019;471:313-327 pubmed publisher
	Zankov D, Salloum F, Jiang M, Tseng G. Chronic in vivo angiotensin II administration differentially modulates the slow delayed rectifier channels in atrial and ventricular myocytes. Heart Rhythm. 2019;16:108-116 pubmed publisher
	Lv Y, Wang Y, Zhu X, Zhang H. Aldosterone downregulates delayed rectifier potassium currents through an angiotensin type 1 receptor-dependent mechanism. Am J Transl Res. 2018;10:1413-1421 pubmed
	Lai Y, Li N, Lawrence W, Wang S, Levine A, Burchhardt D, et al. Myocardial remodeling and susceptibility to ventricular tachycardia in a model of chronic epilepsy. Epilepsia Open. 2018;3:213-223 pubmed publisher
	Meisel E, Tobelaim W, Dvir M, Haitin Y, Peretz A, Attali B. Inactivation gating of Kv7.1 channels does not involve concerted cooperative subunit interactions. Channels (Austin). 2018;12:89-99 pubmed publisher
	Masuda K, Takanari H, Morishima M, Ma F, Wang Y, Takahashi N, et al. Testosterone-mediated upregulation of delayed rectifier potassium channel in cardiomyocytes causes abbreviation of QT intervals in rats. J Physiol Sci. 2018;68:759-767 pubmed publisher
	Kanner S, Morgenstern T, Colecraft H. Sculpting ion channel functional expression with engineered ubiquitin ligases. elife. 2017;6: pubmed publisher
	Tommiska J, Känsäkoski J, Skibsbye L, Vaaralahti K, Liu X, Lodge E, et al. Two missense mutations in KCNQ1 cause pituitary hormone deficiency and maternally inherited gingival fibromatosis. Nat Commun. 2017;8:1289 pubmed publisher
	Piccini I, Fehrmann E, Frank S, Müller F, Greber B, Seebohm G. Adrenergic Stress Protection of Human iPS Cell-Derived Cardiomyocytes by Fast Kv7.1 Recycling. Front Physiol. 2017;8:705 pubmed publisher
	Moreno C, Oliveras A, Bartolucci C, Munoz C, de la Cruz A, Peraza D, et al. D242N, a KV7.1 LQTS mutation uncovers a key residue for IKs voltage dependence. J Mol Cell Cardiol. 2017;110:61-69 pubmed publisher
	Bientinesi R, Mancuso C, Martire M, Bassi P, Sacco E, Currò D. KV7 channels in the human detrusor: channel modulator effects and gene and protein expression. Naunyn Schmiedebergs Arch Pharmacol. 2017;390:127-137 pubmed publisher
	Opthof T, Remme C, Jorge E, Noriega F, Wiegerinck R, Tasiam A, et al. Cardiac activation-repolarization patterns and ion channel expression mapping in intact isolated normal human hearts. Heart Rhythm. 2017;14:265-272 pubmed publisher

image

image 1 :

Western blot analysis of rat heart membranes: - 1. Anti-KCNQ1 Antibody (#APC-022), (1:200). 2. Anti-KCNQ1 Antibody, preincubated with KCNQ1 Blocking Peptide (#BLP-PC022).

image 2 :

Expression of KCNQ1 (KV7.1) and KCNQ5 (KV7.5) in rat smooth muscle cells. - Immunocytochemical staining of rat smooth muscle cells from LCA and RCA using Anti-KCNQ1 Antibody (#APC-022) and Anti-KCNQ5 Antibody (#APC-155) shows that the expression of both channels is higher in LCA than in RCA.Adapted from Morales-Cano D. et al. (2015) with permission of the European Society of Cardiology.

product information

CAT :

APC-022

SKU :

APC-022-CF_0.2 ml

Product Name :

Anti-KCNQ1 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P51787

Applications :

IC IF IHC IP WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PC022

Homology :

Rat, mouse - 14/16 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

(C)TYEQLTVPRRGPDEGS, corresponding to amino acid residues 661-676 of human KCNQ1

Product Page - Scientific background :

KCNQ1 (KV7.1) is part of a voltage-gated K+ family that includes 5 other members (KCNQ1-5). The channel has been intensively studied since it was found that together with MinK (Isk) it underlay the cardiac Iks current that controls the duration of the action potential of the human heart. Indeed mutations on either KV7.1 or MinK can be responsible for long QT syndrome, a cardiac disorder that causes arrhythmias and sudden death. Although KV7.1 expression and function has been mainly investigated in relation with cardiac function the channel is widely distributed in epithelial tissues. There it has been implicated in physiological functions such as the regulation of acid secretion in the stomach and Cl- secretion into the colon.

Applications may also work in :

IC IF IHC IP WB

Supplier :

Alomone Labs

Target :

KV7.1, Voltage-gated potassium channel QKT subfamily member 1, KvLQT1

Short Description :

A Rabbit Polyclonal Antibody to KCNQ1 (KV7.1) Channel

Long Description :

Anti-KCNQ1 Antibody (#APC-022) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot, immunoprecipitaion, immunohistochemistry and immunocytochemistry applications. It has been designed to recognize KV7.1 channel from rat, human, and mouse samples.

Negative Control :

BLP-PC022

Positive Control :

Synonyms :

KV7.1, Voltage-gated potassium channel QKT subfamily member 1, KvLQT1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

KCNQ1

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IHC ICC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage