Guinea pig Anti-KCNQ1 Antibody | Alomone Labs APC-022-GP product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Guinea pig Anti-KCNQ1 Antibody

catalog :

APC-022-GP

clonality :

polyclonal

host :

guinea-pigs

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Gou X, Hu T, Gou Y, Li C, Yi M, Jia M. Specific protein kinase C isoform exerts chronic inhibition on the slowly activating delayed-rectifier potassium current by affecting channel trafficking. Channels (Austin). 2021;15:262-272 pubmed publisher

image

image 1 :

Western blot analysis of rat heart membrane (lanes 1 and 3), rat spleen lysate (lanes 2 and 4), mouse heart membrane (lanes 5 and 6) and human Colo205 cell line lysate (lanes 7 and 8): - 1,2,5,7. Guinea pig Anti-KCNQ1 Antibody (#APC-022-GP), (1:200).3,4,6,8. Guinea pig Anti-KCNQ1 Antibody, preincubated with KCNQ1 Blocking Peptide (#BLP-PC022).

image 2 :

Expression of KCNQ1 in mouse heart - Immunohistochemical staining of mouse heart frozen section using Guinea pig Anti-KCNQ1 Antibody (#APC-022-GP), (1:200). A. KCNQ1 staining (red) appears in muscle fiber membranes (arrows). B. Nuclei are stained using DAPI counterstain (blue). C. Merged image of panels A and B.

product information

CAT :

APC-022-GP

SKU :

APC-022-GP-CF_0.2 ml

Product Name :

Guinea pig Anti-KCNQ1 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P51787

Applications :

IHC WB

Reactivity :

Human Rat Mouse

Host :

Guinea Pig

Blocking Peptide :

BLP-PC022

Homology :

Rat, mouse - 14/16 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Guinea pig total IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

(C)TYEQLTVPRRGPDEGS, corresponding to amino acid residues 661-676 of human KCNQ1

Product Page - Scientific background :

KCNQ1 (KV7.1) is part of a voltage-gated K+ family that includes 5 other members (KCNQ1-5).The channel has been intensively studied since it was found that together with MinK (Isk) it is responsible for the cardiac Iks current that controls the duration of the action potential of the human heart. Indeed, mutations in either KV7.1 or MinK can be responsible for long QT syndrome, a cardiac disorder that causes arrhythmias and sudden death1-3.Although KV7.1 expression and function have been mainly investigated in relation with cardiac function the channel is widely distributed in epithelial tissues where it is involved in physiological functions such as the regulation of acid secretion in the stomach and Cl- secretion into the colon1-3.

Applications may also work in :

IHC WB

Supplier :

Alomone Labs

Target :

KV7.1, Voltage-gated potassium channel QKT subfamily member 1, KVLQT1

Short Description :

A Guinea Pig Polyclonal Antibody to KCNQ1 (KV7.1) Channel

Long Description :

Guinea pig Anti-KCNQ1 Antibody (#APC-022-GP, formerly AGP-050), raised in guinea pigs, is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize KV7.1 channel from mouse, rat, and human samples. The antigen used to immunize guinea pigs is the same as Anti-KCNQ1 Antibody (#APC-022) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation, etc.

Negative Control :

BLP-PC022

Positive Control :

Synonyms :

KV7.1, Voltage-gated potassium channel QKT subfamily member 1, KVLQT1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

KCNQ1

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IHC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:200

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage