Anti-Kir6.2 Antibody | Alomone Labs APC-020 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Kir6.2 Antibody

catalog :

APC-020

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

Alomone Labs product webpage

citations: 5

Reference
Akyüz E, Villa C, Beker M, Elibol B. Unraveling the Role of Inwardly Rectifying Potassium Channels in the Hippocampus of an Aβ(1-42)-Infused Rat Model of Alzheimer's Disease. Biomedicines. 2020;8: pubmed publisher
Gerzanich V, Stokum J, Ivanova S, Woo S, Tsymbalyuk O, Sharma A, et al. SUR1, TRPM4 and KIR6.2 - role in hemorrhagic progression of contusion. J Neurotrauma. 2018;: pubmed publisher
Castro L, Noelia M, Vidal Jorge M, Sanchez Ortiz D, Gándara D, Martínez Sáez E, et al. Kir6.2, the Pore-Forming Subunit of ATP-Sensitive K+ Channels, Is Overexpressed in Human Posttraumatic Brain Contusions. J Neurotrauma. 2018;: pubmed publisher
Yang J, Cheng R, Cheng P, Wang Y, Huang R. KATP Channels Mediate Differential Metabolic Responses to Glucose Shortage of the Dorsomedial and Ventrolateral Oscillators in the Central Clock. Sci Rep. 2017;7:640 pubmed publisher
Gangadhariah M, Dieckmann B, Lantier L, Kang L, Wasserman D, Chiusa M, et al. Cytochrome P450 epoxygenase-derived epoxyeicosatrienoic acids contribute to insulin sensitivity in mice and in humans. Diabetologia. 2017;60:1066-1075 pubmed publisher

image

image 1 :

Western blot analysis of rat pancreas membranes: - 1. Anti-Kir6.2 Antibody (#APC-020), (1:200). 2. Anti-Kir6.2 Antibody, preincubated with Kir6.2 Blocking Peptide (#BLP-PC020).

image 2 :

Expression of Kir6.2 in rat pancreas - Immunohistochemical staining of rat pancreas using Anti-Kir6.2 Antibody (#APC-020). A. Strong granular staining in a number of cells within the Islets of Langerhans is readily detected (red). B. The negative control slide shows no staining.

image 3 :

Expression of Kir6.2 in rat suprachiasmatic nucleus sections - Immunohistochemical staining of rat suprachiasmatic nucleus sections (SCN) using Anti-Kir6.2 Antibody (#APC-020). Kir6.2 staining (green) colocalizes with Arginine-Vasopressin (AVP) staining (red) in the soma (middle panel) and in varicosities along the process (right panel).Adapted from Yang J.J. et al. (2017) Sci. Rep. 7 640. with permission of SPRINGER NATURE.

product information

CAT :

APC-020

SKU :

APC-020-CF_0.2 ml

Product Name :

Anti-Kir6.2 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P70673

Applications :

IC IF IFC IHC IP WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PC020

Homology :

Mouse - identical; human - 12/14 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminal domain

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)SVAVAKAKPKFSIS, corresponding to amino acid residues 372-385 of rat Kir6.2

Product Page - Scientific background :

Kir6.2 is a member of the inward rectifier K+ channels (Kir channels), a large family of voltage-independent K+ channels largely involved in stabilization of the membrane resting potential and in K+ transport across membranes. Kir channels can be modulated by a variety of intracellular agents such as protons, GTP-binding proteins and adenine nucleotides. The ATP-sensitive channel (KATP) is especially important since it couples cellular metabolism (intracellular ATP levels) with cell excitability. KATP channels have been described in pancreatic b-cells, neurons, heart, skeletal and smooth muscle. The KATP channel is composed of a Kir6.2 or Kir6.1 subunit and a sulphonylurea receptor (SUR) subunit. The pancreatic KATP channel for example, is composed of a complex of Kir6.2 and SUR1 subunits, while the cardiac KATP channel is composed of Kir6.2 and SUR2A complexes. Impaired b-cell KATP channel function due to mutations in either Kir6.2 or SUR1 subunits has been linked to the recessive autosomal disorder called persistent hyperinsulinemic hypoglycemia of infancy (PHHI). In addition, a Kir6.2 variant has recently been linked to an increased risk of developing type-2 diabetes.

Applications may also work in :

IC IF IFC IHC IP WB

Supplier :

Alomone Labs

Target :

Inward rectifier K+ channel Kir6.2, ATP-sensitive inward rectifier potassium channel 11, KCNJ11, BIR, TNDM3

Short Description :

A Rabbit Polyclonal Antibody to Kir6.2 Channel

Long Description :

Anti-Kir6.2 Antibody (#APC-020) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunocytochemistry, and immunohistochemistry applications. It has been designed to recognize Kir6.2 from human, rat, and mouse samples. The antibody is specific for Kir6.2 and does not cross react with Kir6.1.

Negative Control :

BLP-PC020

Positive Control :

Synonyms :

Inward rectifier K+ channel Kir6.2, ATP-sensitive inward rectifier potassium channel 11, KCNJ11, BIR, TNDM3

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

KCNJ11

AB Specifictiy :

The antibody is specific for Kir6.2 and does not cross react with Kir6.1.

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

KO-Validated :

yes

Cited Application :

IHC ICC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage