Guinea pig Anti-Kir6.2 Antibody | Alomone Labs APC-020-GP product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Guinea pig Anti-Kir6.2 Antibody

catalog :

APC-020-GP

clonality :

polyclonal

host :

guinea-pigs

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 2

Reference
Kuo Y, Lin J, Lin Y, Chen J, Kuo Y, Chen P, et al. Glibenclamide restores dopaminergic reward circuitry in obese mice through interscauplar brown adipose tissue. Psychoneuroendocrinology. 2020;118:104712 pubmed publisher
Shen H, Liu W, Geng Q, Li H, Lu M, Liang P, et al. Age-Dependent Up-Regulation of HCN Channels in Spiral Ganglion Neurons Coincide With Hearing Loss in Mice. Front Aging Neurosci. 2018;10:353 pubmed publisher

image

image 1 :

Western blot analysis of rat heart membranes (lanes 1 and 3) and mouse heart lysate (lanes 2 and 4): - 1-2. Guinea pig Anti-Kir6.2 Antibody (#APC-020-GP), (1:500).3-4. Guinea pig Anti-Kir6.2 Antibody, preincubated with Kir6.2 Blocking Peptide (#BLP-PC020).

image 2 :

Expression of Kir6.2 in mouse hippocampus - Immunohistochemical staining of perfusion-fixed frozen mouse brain sections using Guinea pig Anti-Kir6.2 Antibody (#APC-020-GP), (1:300), followed by goat-anti-guinea pig-Cy3 antibody (red staining). Kir6.2 staining appears in the hilus region in neuronal outlines (arrows). Nuclei are stained with DAPI (blue).

product information

CAT :

APC-020-GP

SKU :

APC-020-GP-CF_0.2 ml

Product Name :

Guinea pig Anti-Kir6.2 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P70673

Applications :

IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Guinea Pig

Blocking Peptide :

BLP-PC020

Homology :

Mouse - identical; human - 12/14 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Guinea pig total IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminal domain

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)SVAVAKAKPKFSIS, corresponding to amino acid residues 372-385 of rat Kir6.2

Product Page - Scientific background :

Kir6.2 is a member of the inward rectifier K+ channels (Kir channels), a large family of voltage-independent K+ channels largely involved in stabilization of the membrane resting potential and in K+ transport across membranes. Kir channels can be modulated by a variety of intracellular agents such as protons, GTP-binding proteins and adenine nucleotides. The ATP-sensitive channel (KATP) is especially important since it couples cellular metabolism (intracellular ATP levels) with cell excitability. KATP channels have been described in pancreatic b-cells, neurons, heart, skeletal and smooth muscle. The KATP channel is composed of a Kir6.2 or Kir6.1 subunit and a sulphonylurea receptor (SUR) subunit. The pancreatic KATP channel for example, is composed of a complex of Kir6.2 and SUR1 subunits, while the cardiac KATP channel is composed of Kir6.2 and SUR2A complexes. Impaired b-cell KATP channel function due to mutations in either Kir6.2 or SUR1 subunits has been linked to the recessive autosomal disorder called persistent hyperinsulinemic hypoglycemia of infancy (PHHI). In addition, a Kir6.2 variant has recently been linked to an increased risk of developing type-2 diabetes.

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

Inward rectifier K+ channel Kir6.2, ATP-sensitive inward rectifier potassium channel 11, KCNJ11, BIR, TNDM3

Short Description :

A Guinea Pig Polyclonal Antibody to Kir6.2 Channel

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat Kir6.2. Guinea pig Anti-Kir6.2 Antibody (#APC-020-GP, formerly AGP-067) raised in guinea pig can be used in western blot and immunohistochemistry applications. It has been designed to recognize Kir6.2 from human, rat and mouse samples. The antigen used to immunize guinea pigs is the same as Anti-Kir6.2 Antibody (#APC-020) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation, etc.

Negative Control :

BLP-PC020

Positive Control :

Synonyms :

Inward rectifier K+ channel Kir6.2, ATP-sensitive inward rectifier potassium channel 11, KCNJ11, BIR, TNDM3

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

KCNJ11

AB Specifictiy :

The antibody is specific for Kir6.2 and does not cross-react with Kir6.1.

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:300

Application Dilutions: Western blot wb :

1:200-1:500

more info or order :

Alomone Labs product webpage