Anti-KCNC4 (KV3.4) Antibody | Alomone Labs APC-019 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-KCNC4 (KV3.4) Antibody

catalog :

APC-019

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

Alomone Labs product webpage

citations: 8

Reference
Yeap J, Sathyaprakash C, Toombs J, Tulloch J, Scutariu C, Rose J, et al. Reducing voltage-dependent potassium channel Kv3.4 levels ameliorates synapse loss in a mouse model of Alzheimer's disease. Brain Neurosci Adv. 2022;6:23982128221086464 pubmed publisher
Zhang W, Cao H, Li Y, Fu X, Zhang Y. Peripheral ablation of type III adenylyl cyclase induces hyperalgesia and eliminates KOR-mediated analgesia in mice. JCI Insight. 2022;7: pubmed publisher
Ianevski A, Zusinaite E, Shtaida N, Kallio Kokko H, Valkonen M, Kantele A, et al. Low Temperature and Low UV Indexes Correlated with Peaks of Influenza Virus Activity in Northern Europe during 2010⁻2018. Viruses. 2019;11: pubmed publisher
Song M, Park S, Park J, Byun J, Jin H, Seo S, et al. Kv3.1 and Kv3.4, Are Involved in Cancer Cell Migration and Invasion. Int J Mol Sci. 2018;19: pubmed publisher
Muqeem T, Ghosh B, Pinto V, Lepore A, Covarrubias M. Regulation of Nociceptive Glutamatergic Signaling by Presynaptic Kv3.4 Channels in the Rat Spinal Dorsal Horn. J Neurosci. 2018;38:3729-3740 pubmed publisher
Hartmann S, Zheng F, Kyncl M, Karch S, Voelkl K, Zott B, et al. Î²-Secretase BACE1 Promotes Surface Expression and Function of Kv3.4 at Hippocampal Mossy Fiber Synapses. J Neurosci. 2018;38:3480-3494 pubmed publisher
Sakai A, Saitow F, Maruyama M, Miyake N, Miyake K, Shimada T, et al. MicroRNA cluster miR-17-92 regulates multiple functionally related voltage-gated potassium channels in chronic neuropathic pain. Nat Commun. 2017;8:16079 pubmed publisher
Wolff M, Czorlich P, Nagaraj C, SchnÃ¶bel Ehehalt R, Li Y, Kwapiszewska G, et al. Amitriptyline and carbamazepine utilize voltage-gated ion channel suppression to impair excitability of sensory dorsal horn neurons in thin tissue slice: An in vitro study. Neurosci Res. 2016;109:16-27 pubmed publisher

image

image 1 :

Western blot analysis of rat brain membranes: - 1. Anti-KCNC4 (KV3.4) Antibody (#APC-019), (1:200).2. Anti-KCNC4 (KV3.4) Antibody, preincubated with KCNC4/Kv3.4 Blocking Peptide (#BLP-PC019).

image 2 :

Western blot analysisof rat brain membranes: - 1.Anti-KCNC4 (KV3.4)Antibody (#APC-019) (1:200).2. Anti-KCNC4 (KV3.4) Antibody preincubated with the negative control antigen.

product information

CAT :

APC-019

SKU :

APC-019-CF_0.2 ml

Product Name :

Anti-KCNC4 (KV3.4) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q63734

Applications :

IC IF IHC IP WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PC019

Homology :

Human - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

EAGDD ERELA LQRLG PHEG(C), corresponding to amino acid residues 177-195 of rat KCNC4

Product Page - Scientific background :

KV3.4 is a member of the voltage-gated K+ channel superfamily. Together with the related proteins KV3.1, KV3.2 and KV3.3 they constitute the Shaw type subfamily family.1As with all Kv channels, KV3.4 possesses the signature structure of the voltage-dependent K+ channels: six membrane-spanning domains with intracellular N and C termini. The functional KV channel is a tetramer that can either be a homomer or a heteromer of KV3 subunits.2Kv3 subfamily members inactivate very rapidly and therefore are thought to play a role in the repolarization of action potentials and to facilitate repetitive high frequency firing.2Kv3.4 expression is wide and the channel can be found in brain, skeletal muscle, prostate and pancreas among others.KV3.4 subunits have been implicated recently in the response mechanism to chronic hypoxia and the etiology of Alzheimer's and Parkinson's diseases. In addition, KV3.4 was found to associate with the auxiliary subunit KCNE3 (MirP2) in skeletal muscle. A mutation in KCNE3 (R83H) has been associated with an inherited form of periodic paralysis (Thyrotoxic hypokalemic periodic paralysis) that is caused by the altered physiological function of the KV3.4 channel.3Toxins from sea anemone, BDS-I (#STB-400), (47 nM) and BDS-II (#B-450), (56 nM), are potent blockers of KV3.4 channels.4

Applications may also work in :

IC IF IHC IP WB

Supplier :

Alomone Labs

Target :

Potassium voltage-gated channel subfamily C member 4, Raw3, KSHIIIC

Short Description :

A Rabbit Polyclonal Antibody to KCNC4 (KV3.4) Channel

Long Description :

Anti-KCNC4 (KV3.4) Antibody (#APC-019) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunoprecipitation, immunocytochemistry, and immunohistochemistry applications. It has been designed to recognize KV3.4 from human, rat, and mouse samples.

Negative Control :

BLP-PC019

Positive Control :

Synonyms :

Potassium voltage-gated channel subfamily C member 4, Raw3, KSHIIIC

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

KCNC4

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage