Anti-KV4.3 Antibody | Alomone Labs APC-017 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-KV4.3 Antibody

catalog :

APC-017

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

Alomone Labs product webpage

citations: 17

Reference
Zhang W, Cao H, Li Y, Fu X, Zhang Y. Peripheral ablation of type III adenylyl cyclase induces hyperalgesia and eliminates KOR-mediated analgesia in mice. JCI Insight. 2022;7: pubmed publisher
Kabakov A, Sengun E, Lu Y, Roder K, Bronk P, Baggett B, et al. Three-Week-Old Rabbit Ventricular Cardiomyocytes as a Novel System to Study Cardiac Excitation and EC Coupling. Front Physiol. 2021;12:672360 pubmed publisher
Liu M, Liu H, Parthiban P, Kang G, Shi G, Feng F, et al. Inhibition of the unfolded protein response reduces arrhythmia risk after myocardial infarction. J Clin Invest. 2021;131: pubmed publisher
Haddjeri Hopkins A, Tapia M, Ramírez Franco J, Tell F, Marquèze Pouey B, Amalric M, et al. Refining the Identity and Role of Kv4 Channels in Mouse Substantia Nigra Dopaminergic Neurons. Eneuro. 2021;8: pubmed publisher
Götz S, Bribian A, López Mascaraque L, Gotz M, Grothe B, Kunz L. Heterogeneity of astrocytes: Electrophysiological properties of juxtavascular astrocytes before and after brain injury. Glia. 2021;69:346-361 pubmed publisher
Xuan L, Zhu Y, Liu Y, Yang H, Wang S, Li Q, et al. Up-regulation of miR-195 contributes to cardiac hypertrophy-induced arrhythmia by targeting calcium and potassium channels. J Cell Mol Med. 2020;24:7991-8005 pubmed publisher
Kim K, Lee S, Yoon S, Kim Y, Jeong H, Lee S, et al. Kv4.1, a Key Ion Channel For Low Frequency Firing of Dentate Granule Cells, Is Crucial for Pattern Separation. J Neurosci. 2020;40:2200-2214 pubmed publisher
Carll A, Salatini R, Pirela S, Wang Y, Xie Z, Lorkiewicz P, et al. Inhalation of printer-emitted particles impairs cardiac conduction, hemodynamics, and autonomic regulation and induces arrhythmia and electrical remodeling in rats. Part Fibre Toxicol. 2020;17:7 pubmed publisher
Kilfoil P, Chapalamadugu K, Hu X, Zhang D, Raucci F, Tur J, et al. Metabolic regulation of Kv channels and cardiac repolarization by Kvβ2 subunits. J Mol Cell Cardiol. 2019;137:93-106 pubmed publisher
Imai R, Yokota S, Horita S, Ueta Y, Maejima Y, Shimomura K. Excitability of oxytocin neurons in paraventricular nucleus is regulated by voltage-gated potassium channels Kv4.2 and Kv4.3. Biosci Biotechnol Biochem. 2019;83:202-211 pubmed publisher
Cembrowski M, Wang L, Lemire A, Copeland M, DiLisio S, Clements J, et al. The subiculum is a patchwork of discrete subregions. elife. 2018;7: pubmed publisher
Huang Y, Yang J, Xie W, Li Q, Zeng Z, Sui H, et al. A novel KCND3 mutation associated with early-onset lone atrial fibrillation. Oncotarget. 2017;8:115503-115512 pubmed publisher
Kim J, Park J, Kang T. TRPC6-mediated ERK1/2 Activation Regulates Neuronal Excitability via Subcellular Kv4.3 Localization in the Rat Hippocampus. Front Cell Neurosci. 2017;11:413 pubmed publisher
Rizwan A, Zhan X, Zamponi G, Turner R. Long-Term Potentiation at the Mossy Fiber-Granule Cell Relay Invokes Postsynaptic Second-Messenger Regulation of Kv4 Channels. J Neurosci. 2016;36:11196-11207 pubmed
Liu X, Zhang Y, Du W, Liang H, He H, Zhang L, et al. MiR-223-3p as a Novel MicroRNA Regulator of Expression of Voltage-Gated K+ Channel Kv4.2 in Acute Myocardial Infarction. Cell Physiol Biochem. 2016;39:102-14 pubmed publisher
Argenziano M, Tiscornia G, Moretta R, Casal L, Potilinski C, Amorena C, et al. Arrhythmogenic effect of androgens on the rat heart. J Physiol Sci. 2017;67:217-225 pubmed publisher
Wolff M, Czorlich P, Nagaraj C, SchnÃ¶bel Ehehalt R, Li Y, Kwapiszewska G, et al. Amitriptyline and carbamazepine utilize voltage-gated ion channel suppression to impair excitability of sensory dorsal horn neurons in thin tissue slice: An in vitro study. Neurosci Res. 2016;109:16-27 pubmed publisher

image

image 1 :

Western blot analysis of rat brain membranes: - 1. Anti-KV4.3 Antibody (#APC-017), (1:200).2. Anti-KV4.3 Antibody, preincubated with Kv4.3 Blocking Peptide (#BLP-PC017).

image 2 :

Expression of KV4.3 in rat hippocampal CA1 region - Immunohistochemical staining of perfusion-fixed frozen rat brain sections using Anti-KV4.3 Antibody (#APC-017), (1:400), followed by donkey anti-rabbit-Cy3 antibody (red). Staining appears in neurons of the CA1 hippocampal region (arrow). Nuclei are stained with DAPI (blue).

product information

CAT :

APC-017

SKU :

APC-017-CF_0.2 ml

Product Name :

Anti-KV4.3 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

O60577

Applications :

IC IF IHC IP WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PC017

Homology :

Rat, rabbit - identical; mouse - 16/17 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

(C)NEALELTGTPEEEHMGK, corresponding to amino acid residues 451-468 of human KV4.3

Product Page - Scientific background :

KV4.3 is a voltage-dependent K+ channel that belongs to the Shal channel subfamily and includes two other members: KV4.1 and KV4.2.KV4.3 possesses the signature structure of the voltage-dependent K+ channels; six membrane-spanning domains with intracellular N and C termini. As with other members of the voltage-gated K+ channel superfamily, the functional channel is a tetramer that can be composed of more than one member of the Shal subfamily, i.e. heterotetramers of KV4.1 and KV4.2.The KV4 channels are characterized by activation at subthreshold membrane potentials, rapid inactivation and fast recovery from inactivation compared with other voltage-dependent K+ channels. This type of current is known as transient A-type K+ currents.1The biophysical properties of the KV4.3 subunit can be modified by its association with auxiliary β subunits such as the KChIP family that increases current densities and accelerates both the inactivation and the recovery time.KV4.3 is highly expressed in the brain where it has a key role in shaping the action potential and firing frequency of neurons. In the heart, together with KV4.2, it underlies the fast inactivating and recovering cardiac transient outward current Ito.2,3 The channel is also expressed in smooth muscle cells of several organs such as myometrium, lung and colon where its function has not been completely elucidated.Several toxins from spider venoms are potent blockers (affecting the channels in the nanomolar range) of KV4.3 channels. Among these the most potent and selective are Phrixotoxin-1 (#STP-700) (28 nM) and Phrixotoxin-2 (#STP-710) (71 nM).4

Applications may also work in :

IC IF IHC IP WB

Supplier :

Alomone Labs

Target :

Voltage-gated K+ channel subfamily D member 3, KCND3

Short Description :

A Rabbit Polyclonal Antibody to KV4.3 Channel

Long Description :

Anti-KV4.3 Antibody (#APC-017) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot, immunoprecipitation, immunocytochemistry, and immunohistochemistry applications. It has been designed to recognize KV4.3 from human, rat, and mouse samples.

Negative Control :

BLP-PC017

Positive Control :

Synonyms :

Voltage-gated K+ channel subfamily D member 3, KCND3

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

KCND3

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IP IHC ICC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:400

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage