Anti-KCNH2 (erg1) Antibody | Alomone Labs APC-016 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-KCNH2 (erg1) Antibody

catalog :

APC-016

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

Alomone Labs product webpage

citations: 9

Reference
Della Flora Nunes G, Wilson E, Marziali L, Hurley E, Silvestri N, He B, et al. Prohibitin 1 is essential to preserve mitochondria and myelin integrity in Schwann cells. Nat Commun. 2021;12:3285 pubmed publisher
Shults N, Rybka V, Suzuki Y, Brelidze T. Increased Smooth Muscle Kv11.1 Channel Expression in Pulmonary Hypertension and Protective Role of Kv11.1 Channel Blocker Dofetilide. Am J Pathol. 2020;190:48-56 pubmed publisher
Foo B, Barbier C, Guo K, Vasantharuban J, Lukacs G, Shrier A. Mutation-specific peripheral and ER quality control of hERG channel cell-surface expression. Sci Rep. 2019;9:6066 pubmed publisher
Han M, Zhao M, Cheng C, Huang Y, Han S, Li W, et al. Lamin A mutation impairs interaction with nucleoporin NUP155 and disrupts nucleocytoplasmic transport in atrial fibrillation. Hum Mutat. 2019;40:310-325 pubmed publisher
Wang Y, Shen T, Fang P, Zhou J, Lou K, Cen Z, et al. The role and mechanism of chaperones Calnexin/Calreticulin in which ALLN selectively rescues the trafficking defective of HERG-A561V mutation. Biosci Rep. 2018;38: pubmed publisher
Hu L, Wang B, Zhang Y. Serotonin 5-HT6 receptors affect cognition in a mouse model of Alzheimer's disease by regulating cilia function. Alzheimers Res Ther. 2017;9:76 pubmed publisher
Liu Y, Li D, Nie D, Liu S, Qiu F, Liu M, et al. Arsenic trioxide and angiotensin II have inhibitory effects on HERG protein expression: Evidence for the role of PML SUMOylation. Oncotarget. 2017;8:45447-45458 pubmed publisher
Caballero R, Utrilla R, AmorÃ³s I, Matamoros M, PÃ©rez HernÃ¡ndez M, Tinaquero D, et al. Tbx20 controls the expression of the KCNH2 gene and of hERG channels. Proc Natl Acad Sci U S A. 2017;114:E416-E425 pubmed publisher
Zhang Y, Colenso C, El Harchi A, Cheng H, Witchel H, Dempsey C, et al. Interactions between amiodarone and the hERG potassium channel pore determined with mutagenesis and in silico docking. Biochem Pharmacol. 2016;113:24-35 pubmed publisher

image

image 1 :

Western blot analysis of HEK 293 cell lysate, stably expressing HERG channels: - 1. Anti-KCNH2 (erg1) Antibody (1:200).2. Anti-KCNH2 (erg1) Antibody, preincubated with KCNH2/erg1 Blocking Peptide (#BLP-PC016).

image 2 :

Peptide (CY)EEL PAGAP ELPQD GPT corresponding to residues 1122-1137 of rat KV11.1 (erg1) (AccessionO08962). Intracellular C-terminal part.

image 3 :

Western blot analysisof HEK 293 cell lysate stably expressing HERG channels: - 1.Anti-KCNH2(erg1) Antibody(1:200).2. Anti-KCNH2(erg1) Antibody preincubated with the negative control antigen.

product information

CAT :

APC-016

SKU :

APC-016-CF_0.2 ml

Product Name :

Anti-KCNH2 (erg1) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

O08962

Applications :

IC IF IHC IP WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PC016

Homology :

Mouse - identical; human, dog, rabbit - 14/16 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminal domain

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)EELPAGAPELPQDGPT, corresponding to residues 1122-1137 of rat KV11.1 (erg1)

Product Page - Scientific background :

The KV11.1 (HERG) channel is a member of the ether-a-go-go (EAG) subfamily of voltage-dependent K+ channels that includes the related proteins KV11.2 and KV11.3 (erg2 and erg3). KV11.1 possesses the signature structure of the voltage-dependent K+ channels: six membrane-spanning domains and intracellular N- and C-termini.The KV11.1 current is characterized by strong inward rectification with slow activation and very rapid inactivation kinetics. The channel is expressed in the brain and heart (where it underlies the IKr current) and has a central role in mediating repolarization of action potentials.1,2Mutations in the KV11.1 channel cause inherited long QT syndrome (LQTS) or abnormalities in the repolarization of the heart that are associated with life-threatening arrhythmias and sudden death. All the identified KV11.1 mutations produce loss of function of the channel via several cellular mechanisms ranging from alterations of gating properties, alterations of channel permeability/selectivity and alterations in intracellular channel trafficking that decreases the number of channels that reach the cell membrane.1,2 Recently, drug-induced forms of LQTS have been reported for a wide range of non-cardiac drugs including antihistamines, psychoactive agents and antimicrobials. All these drugs potently block the KV11.1 channel as an unintended side effect, prompting regulatory drug agencies to issue recommendations for the testing of new drugs for their potential KV11.1 blocking effect.In addition, KV11.1 expression was found to be upregulated in several tumor cell lines of different histogenesis, suggesting that it confers the cells some advantage in cell proliferation. Indeed, in several studies it has been shown that inhibition of the KV11.1 current leads to a decrease in tumor cell proliferation.3Several toxins from scorpion venoms are potent blockers (affecting the channels in the nanomolar range) of KV11.1 channels. Among these the most potent and selective are Ergtoxin-1 (16 nM)4 and BeKM-1 (3 nM).5 In addition, the methanesulfonanilide class III antiarrhythmic agent E-4031 also blocks KV11.1 channel in the nanomolar range (7.7 nM).6

Applications may also work in :

IC IF IHC IP WB

Supplier :

Alomone Labs

Target :

KV11.1, Potassium voltage-gated channel subfamily H member 2, Ether-a-go-go-related channel 1, Eag-related protein 1, ERG-1, HERG

Short Description :

A Rabbit Polyclonal Antibody to KV11.1 (ERG-1) Channel

Long Description :

Anti-KCNH2 (erg1) Antibody (#APC-016) is a highly specific antibody directed against an epitope of the rat KV11.1 channel. The antibody can be used in western blot, immunohistochemistry, immunocytochemistry, and immunoprecipitation applications. It has been designed to recognize KV11.1 from human, rat, and mouse samples.

Negative Control :

BLP-PC016

Positive Control :

Synonyms :

KV11.1, Potassium voltage-gated channel subfamily H member 2, Ether-a-go-go-related channel 1, Eag-related protein 1, ERG-1, HERG

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

KCNH2

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

KO-Validated :

yes

Cited Application :

IP IHC ICC IFC Electrophysiology

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage